PhoN-expressing, lyophilized, recombinant Deinococcus radiodurans cells for uranium bioprecipitation

Journal of Biotechnology

Volumn 154, Issue 4, 2011, Pages 285-290

  Appukuttan, Deepti ^a   Seetharam, Chitra ^a   Padma, N ^a   Rao, Amara Sambasiva ^a   Apte, Shree Kumar ^a  

^a BHABHA ATOMIC RESEARCH CENTRE   (India)

Author keywords

Deinococcus radiodurans; Lyophilized; Recombinant; Uranium

Indexed keywords

ACID PHOSPHATASE; BATCH PROCESS; BIO-PRECIPITATION; CELL MORPHOLOGY; CELL SURFACES; DEINOCOCCUS RADIODURANS; DRY WEIGHT; FLOW-THROUGH SYSTEMS; HIGH LOADINGS; LYOPHILIZED; RADIATION RESISTANT; RECOMBINANT; ROOM TEMPERATURE; SURFACE NUCLEATION; URANIUM CONCENTRATION; URANIUM PRECIPITATION;

BATCH DATA PROCESSING; CELL MEMBRANES; GENE ENCODING; GENETIC ENGINEERING; PHOSPHATASES; RADIOACTIVE WASTES; URANIUM;

CYTOLOGY;

BACTERIUM LYSATE; LYSOSOME ENZYME; PHON PROTEIN; UNCLASSIFIED DRUG; URANIUM;

ARTICLE; BACTERIAL CELL; BACTERIAL LOAD; BACTERIAL SURVIVAL; CELL VIABILITY; COLONY FORMING UNIT; DEINOCOCCUS RADIODURANS; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; FREEZE DRYING; PRECIPITATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; RADIOIMMUNOPRECIPITATION; ROENTGEN SPECTROSCOPY; ROOM TEMPERATURE; SCANNING ELECTRON MICROSCOPY;

BIODEGRADATION, ENVIRONMENTAL; DEINOCOCCUS; FREEZE DRYING; URANIUM;

DEINOCOCCUS; DEINOCOCCUS RADIODURANS;

EID: 79960428257     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.05.002     Document Type: Article

References (21)

1. Appukuttan D., Rao A.S., Apte S.K. Engineering of Deinococcus radiodurans R1 for bioprecipitation of uranium from dilute nuclear waste. Appl. Environ. Microbiol. 2006, 72:7873-7878.
2. Basnakova G., Stephens E.R., Thaller M.C., Rossolini G.M., Macaskie L.E. The use of Escherichia coli bearing a phoN gene for the removal of uranium and nickel from aqueous flows. Appl. Microbiol. Biotechnol. 1998, 50:266-272.
3. Battista J.R. Against all odds: the survival strategies of Deinococcus radiodurans. Annu. Rev. Microbiol. 1997, 51:203-224.
4. Bolton P.G., Dean A.C.R. Phosphatase synthesis in Klebsiella (Aerobacter) aerogenes growing in continuous cultures. Biochem. J. 1972, 127:87-96.
5. Daly M.J. Engineering radiation-resistant bacteria for environmental biotechnology. Curr. Opin. Biotechnol. 2000, 11:280-285.
6. Finch R., Murakami T. Systematics and paragenesis of uranium minerals. Uranium: Mineralogy, Geochemistry and the Environment 1999, Mineralogical Society of America, Washington, DC. P.C. Burns, R. Finch (Eds.).
7. Gadd G.M. Bioremedial potential of microbial mechanisms of metal mobilization and immobilization. Curr. Opin. Biotechnol. 2000, 11:271-279.
8. Jeong B.C., Hawes C., Bonthrone K.M., Macaskie L.E. Localization of enzymically enhanced heavy metal accumulation by Citrobacter sp. and metal accumulation in vitro by liposomes containing entrapped enzyme. Microbiology 1997, 143:2497-2507.
9. Joshi J.M., Pathak P.N., Manchanda V.K. Selective removal of uranium from high-level waste solution employing Tri-n-Butyl phosphate as the extractant. Solvent extraction and ion exchange 2005, 23:663-675.
10. Karnovsky M.J. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 1965, 27:137A-138A. (Abst).
11. Kier L.D., Weppelman R.M., Ames B.N. Resolution and purification of three periplasmic phosphatases of Salmonella typhimurium. J. Bacteriol. 1977, 130:399-410.
12. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951, 193:265-275.
13. Macaskie L.E. An immobilized Cell Bioprocess for the Removal of Heavy Metals from Aqueous Flows. J. Chem. Technol. Biotechnol. 1990, 49:357-379.
14. Macaskie L.E., Jeong B.C., Tolley M.R. Enzymically accelerated biomineralization of heavy metals: application to the removal of americium and plutonium from aqueous flows. FEMS Microbiol. Rev. 1994, 14:351-368.
15. Macaskie L.E., Lloyd J.R., Thomas R.A.P., Tolley M.R. The use of micro-organisms for the remediation of solutions contaminated with actinide elements, other radionuclides, and other organic contaminants generated by nuclear fuel activities. Nucl. Energy 1996, 35(4):257-271.
16. Macaskie L.E., Bonthrone K.M., Yong P., Goddard D.T. Enzymically mediated bioprecipitation of uranium by a Citrobacter sp.: a concerted role for exocellular lipopolysaccharide and associated phosphatase in biomineral formation. Microbiology 2000, 146:1855-1867.
17. Meima R., Lidstrom M.E. Characterization of the minimal replicon of a cryptic Deinococcus radiodurans SARK plasmid and development of versatile Escherichia coli-D. radiodurans shuttle vectors. Appl. Environ. Microbiol. 2000, 66:3856-3867.
18. Heather R., Lara J.C., Schmid A.K., Lidstrom M.E. Involvement of the S-layer proteins Hpi and SlpA in the maintenance of cell envelope integrity in Deinococcus radiodurans R1. Microbiology 2006, 152:2779-2787.
19. Ruggiero C.E., Boukhalfa H., Forsythe J.H., Lack J.G., Hersman L.E., Neu M.P. Actinide and metal toxicity to prospective bioremediation bacteria. Environ. Microbiol. 2005, 7:88-97.
20. Seetharam C., Soundarajan S., Udas A.C., Rao A.S., Apte S.K. Lyophilized, non-viable, recombinant E. coli cells for cadmium bioprecipitation and recovery. Process Biochem. 2009, 44:246-250.
21. Snowman J.W. Downstream Processes: Equipment and Techniques 1988, Alan R. Liss, Inc., New York, pp. 315-351.