Transmission electron microscopy staining methods for the cortex of human hair: A modified osmium method and comparison with other stains

Journal of Microscopy

Volumn 243, Issue 2, 2011, Pages 184-196

  Harland, D P ^a   Vernon, J A ^a   Walls, R J ^a   Woods, J L ^a  

^a AGRESEARCH LTD   (New Zealand)

Author keywords

Hair; Human hair; Keratin fibres; TEM; Transmission electron microscopy

Indexed keywords

HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY; LEAD COMPOUNDS; NITRATES; POTASH; SILVER COMPOUNDS; URANIUM COMPOUNDS;

% REDUCTIONS; CORTEXES; HAIR; HUMAN HAIR; KERATIN FIBERS; OSMIUM TETROXIDE; REDUCTANTS; SCALP HAIR; STAINING METHOD; TEM;

FIBERS;

AMMONIACAL SILVER NITRATE; LEAD; LEAD CITRATE; OSMIUM; PERMANGANATE POTASSIUM; PHOSPHOTUNGSTIC ACID; REAGENT; SILVER DERIVATIVE; SILVER NITRATE; UNCLASSIFIED DRUG; URANYL ACETATE;

ARTICLE; ARTIFACT; CHEMICAL MODIFICATION; CONTROLLED STUDY; EMBEDDING; HAIR ANALYSIS; HAIR TEXTURE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; REDUCTION; REDUCTION OSMICATION; SCALP HAIR; SKIN PENETRATION; STAIN; STAINING; TRANSMISSION ELECTRON MICROSCOPY; WOOL;

EID: 79960415776     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2011.03493.x     Document Type: Article

References (17)

1. Bryson, W.G., Harland, D.P., Caldwell, J.P., et al (2009) Cortical cell types and intermediate filament arrangements correlate with fiber curvature in Japanese human hair. J. Struct. Biol. 166, 46-58.
2. Caldwell, J.P., Mastronarde, D.N., Woods, J.L. & Bryson, W.G. (2005) The three-dimensional arrangement of intermediate filaments in Romney wool cortical cells. J. Struct. Biol. 151, 298-305.
3. Harland, D.P., Caldwell, J.P., Woods, J.L., Walls, R.J. & Bryson, W.G. (2011) Arrangement of trichokeratin intermediate filaments and matrix in the cortex of Merino wool. J. Struct. Biol. 173, 29-37.
4. Hayat, M.A. (1989) Principles and Techniques of Electron Microscopy Biological Applications. Macmillan Press, Basingstoke and London
5. Lewis, P.R. & Knight, D.P. (1977) Staining Methods for Sectioned Material. Elsevier, Amsterdam
6. Maderson, P.F.A. (2003) Mammalian skin evolution: a reevaluation. Exp. Dermatol. 12, 233-236.
7. Nelson, W.G. & Woods, J.L. (1996) An effective method for mounting fibres to allow simple processing, embedding and alignment for sectioning. J. Microsc. 181, 88-90.
8. Orwin, D.F.G., Woods, J.L. & Ranford, S.L. (1984) Cortical cell types and their distribution in wool fibres. Aust. J. Biol. Sci. 37, 237-255.
9. Rogers, G.E. (1959) Electron microscopy of wool. J. Ultrastructure. Res. 2, 309-330.
10. Rogers, G.E. & Filshie, B.K. (1962) Electron staining and fine structure of keratins. Int. Cong. Elect. Micros. 5, O-2.
11. Swift, J.A. (1997) Fundamentals of Human Hair Science. Micelle Press, Weymouth, UK
12. Swift, J.A. & Asquith, S. (1977) The histology of keratin fibers. In Chemistry of Natural Protein Fibers (ed. by R.S. Asquith). John Wiley & Sons, London
13. Swift, J.A. & Bews, B. (1974) The chemistry of human hair cuticle-I. A new method for the physical isolation of cuticle. J. Soc. Cosmet. Chem. 25, 13-22.
14. Swift, J.A. & Bews, B. (1976) The chemistry of human hair cuticle-III. The isolation and amino acid analysis of various subfractions of the cuticle obtained by pronase and trypsin digestion. J. Soc. Cosmet. Chem. 27, 289-300.
15. Tester, D.H. (1987) Fine structure of Cashmere and superfine Merino wool fibers. Text. Res. J. 57, 213-219.
16. Venable, J.H. & Coggeshall, R. (1965) Simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 407-408.
17. Woods, J.L., Harland, D.P., Vernon, J.A., Krsinic, G.L. & Walls, R.J. (2011) Morphology and ultrastructure of antler velvet hair and body hair from red deer (Cervus elaphus). J. Morph. 272, 34-49.