Type 3 fimbriae and biofilm formation are regulated by the transcriptional regulators MrkHI in Klebsiella pneumoniae

Journal of Bacteriology

Volumn 193, Issue 14, 2011, Pages 3453-3460

  Johnson, Jeremiah G ^a   Murphy, Caitlin N ^a   Sippy, Jean ^a   Johnson, Tylor J ^a   Clegg, Steven ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC GMP; PILZ DOMAIN CONTAINING PROTEIN; PROTEIN; TRANSCRIPTINO FACTOR MRKHI; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ARTICLE; BIOFILM; EXTRACELLULAR MATRIX; FIMBRIA; GENE EXPRESSION; GENE INSERTION; IMMUNOBLOTTING; KLEBSIELLA PNEUMONIAE; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION REGULATION; TRANSPOSON; TYPE 3 FIMBRIAE;

AMINO ACID SEQUENCE; BIOFILMS; FIMBRIAE PROTEINS; FIMBRIAE, BACTERIAL; GENE EXPRESSION REGULATION, BACTERIAL; KLEBSIELLA PNEUMONIAE; MOLECULAR SEQUENCE DATA; TRANSCRIPTION, GENETIC;

KLEBSIELLA PNEUMONIAE;

EID: 79960384841     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.00286-11     Document Type: Article

References (30)

1. Blomfield, I. C. 2001. The regulation of pap and type 1 fimbriation in Escherichia coli. Adv. Microb. Physiol. 45:1-49.
2. Boddicker, J. D., R. A. Anderson, J. Jagnow, and S. Clegg. 2006. Signaturetagged mutagenesis of Klebsiella pneumoniae to identify genes that influence biofilm formation on extracellular matrix material. Infect. Immun. 74:4590-4597.
3. de Lorenzo, V., and K. N. Timmis. 1994. Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5- and Tn10-derived minitransposons. Methods Enzymol. 235:386-405.
4. Dickson, C. 2008. Protein techniques: immunoprecipitation, in vitro kinase assays, and Western blotting. Methods Mol. Biol. 461:735-744.
5. Frank, D. N., S. S. Wilson, A. L. St. Amand, and N. R. Pace. 2009. Cultureindependent microbiological analysis of foley urinary catheter biofilms. PLoS One 4:e7811.
6. Gerlach, G. F., B. L. Allen, and S. Clegg. 1988. Molecular characterization of the type 3 (MR/K) fimbriae of Klebsiella pneumoniae. J. Bacteriol. 170: 3547-3553.
7. Gerlach, G. F., B. L. Allen, and S. Clegg. 1989. Type 3 fimbriae among enterobacteria and the ability of spermidine to inhibit MR/K hemagglutination. Infect. Immun. 57:219-224.
8. Gerlach, G. F., S. Clegg, and B. L. Allen. 1989. Identification and characterization of the genes encoding the type 3 and type 1 fimbrial adhesins of Klebsiella pneumoniae. J. Bacteriol. 171:1262-1270.
9. Hickman, J. W., and C. S. Harwood. 2008. Identification of FleQ from Pseudomonas aeruginosa as a c-di-GMP-responsive transcription factor. Mol. Microbiol. 69:376-389.
10. Hickman, J. W., D. F. Tifrea, and C. S. Harwood. 2005. A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels. Proc. Natl. Acad. Sci. U. S. A. 102:14422-14427.
11. Hornick, D. B., B. L. Allen, M. A. Horn, and S. Clegg. 1992. Adherence to respiratory epithelia by recombinant Escherichia coli expressing Klebsiella pneumoniae type 3 fimbrial gene products. Infect. Immun. 60:1577-1588.
12. Hornick, D. B., J. Thommandru, W. Smits, and S. Clegg. 1995. Adherence properties of an mrkD-negative mutant of Klebsiella pneumoniae. Infect. Immun. 63:2026-2032.
13. Jagnow, J., and S. Clegg. 2003. Klebsiella pneumoniae MrkD-mediated biofilm formation on extracellular matrix- and collagen-coated surfaces. Microbiology 149:2397-2405.
14. Johnson, J. G., and S. Clegg. 2010. Role of MrkJ, a phosphodiesterase, in type 3 fimbrial expression and biofilm formation in Klebsiella pneumoniae. J. Bacteriol. 192:3944-3950.
15. Jones, R. N. 2010. Microbial etiologies of hospital-acquired bacterial pneumonia and ventilator-associated bacterial pneumonia. Clin. Infect. Dis. 51(Suppl. 1):S81-S87.
16. Langstraat, J., M. Bohse, and S. Clegg. 2001. Type 3 fimbrial shaft (MrkA) of Klebsiella pneumoniae, but not the fimbrial adhesin (MrkD), facilitates biofilm formation. Infect. Immun. 69:5805-5812.
17. Link, A. J., D. Phillips, and G. M. Church. 1997. Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J. Bacteriol. 179:6228-6237.
18. Mikkelsen, H., G. Ball, C. Giraud, and A. Filloux. 2009. Expression of Pseudomonas aeruginosa CupD fimbrial genes is antagonistically controlled by RcsB and the EAL-containing PvrR response regulators. PLoS One 4:e6018.
19. Miller, V. L., and J. J. Mekalanos. 1988. A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR. J. Bacteriol. 170:2575-2583.
20. Moravek, M., et al. 2004. Colony immunoblot assay for the detection of hemolysin BL enterotoxin producing Bacillus cereus. FEMS Microbiol. Lett. 238:107-113.
21. Nicastro, G. G., A. L. Boechat, C. M. Abe, G. H. Kaihami, and R. L. Baldini. 2009. Pseudomonas aeruginosa PA14 cupD transcription is activated by the RcsB response regulator, but repressed by its putative cognate sensor RcsC. FEMS Microbiol. Lett. 301:115-123.
22. Nordmann, P., G. Cuzon, and T. Naas. 2009. The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria. Lancet Infect. Dis. 9:228-236.
23. Ohana, S., et al. 2005. Spread of a Klebsiella pneumoniae strain producing a plasmid-mediated ACC-1 AmpC beta-lactamase in a teaching hospital admitting disabled patients. Antimicrob. Agents Chemother. 49:2095-2097.
24. O'Toole, G. A., and R. Kolter. 1998. Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol. Microbiol. 30:295-304.
25. O'Toole, G. A., et al. 1999. Genetic approaches to study of biofilms. Methods Enzymol. 310:91-109.
26. Philippe, N., J. P. Alcaraz, E. Coursange, J. Geiselmann, and D. Schneider. 2004. Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria. Plasmid 51:246-255.
27. Pratt, J. T., R. Tamayo, A. D. Tischler, and A. Camilli. 2007. PilZ domain proteins bind cyclic diguanylate and regulate diverse processes in Vibrio cholerae. J. Biol. Chem. 282:12860-12870.
28. Ronald, A. 2002. The etiology of urinary tract infection: traditional and emerging pathogens. Am. J. Med. 113(Suppl. 1A):14S-19S.
29. Rosen, D. A., et al. 2008. Utilization of an intracellular bacterial community pathway in Klebsiella pneumoniae urinary tract infection and the effects of FimK on type 1 pilus expression. Infect. Immun. 76:3337-3345.
30. Schurtz, T. A., D. B. Hornick, T. K. Korhonen, and S. Clegg. 1994. The type 3 fimbrial adhesin gene (mrkD) of Klebsiella species is not conserved among all fimbriate strains. Infect. Immun. 62:4186-4191.