Förster resonance energy transfer demonstrates a flavonoid metabolon in living plant cells that displays competitive interactions between enzymes

FEBS Letters

Volumn 585, Issue 14, 2011, Pages 2193-2198

  Crosby, Kevin C ^a,b   Pietraszewska Bogiel, Anna ^b   Gadella Jr , Theodorus W J ^b   Winkel, Brenda S J ^a  

^a VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Arabidopsis; Enzyme complex; Flavonoid; F rster resonance energy transfer detected by fluorescent lifetime imaging microscopy; Transient expression

Indexed keywords

CHALCONE SYNTHASE; DIHYDROFLAVONOL 4 REDUCTASE; FLAVONOID; FLAVONOID METABOLON; FLAVONOL SYNTHASE 1; MULTIENZYME COMPLEX; UNCLASSIFIED DRUG;

ARABIDOPSIS; ARTICLE; CATALYSIS; CELL METABOLISM; COMPETITIVE INHIBITION; FLUORESCENCE LIFETIME IMAGING MICROSCOPY; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; NONHUMAN; PLANT CELL; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTOPLAST;

ARABIDOPSIS; ARABIDOPSIS PROTEINS; FLAVONOIDS; FLUORESCENCE RESONANCE ENERGY TRANSFER; MICROSCOPY, FLUORESCENCE;

ARABIDOPSIS;

EID: 79960355585     PISSN: 00145793     EISSN: 18733468     Source Type: Journal    
DOI: 10.1016/j.febslet.2011.05.066     Document Type: Article

References (33)

1. R.J. Conrado, J.D. Varner, and M.P. DeLisa Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy Curr. Opin. Biotechnol. 19 2008 492 499
2. P.A. Srere, and J. Ovadi Enzyme-enzyme interactions and their metabolic role FEBS Lett. 268 1990 360 364 (Pubitemid 20241343)
3. B.S.J. Winkel Metabolite channeling and multi-enzyme complexes A.E. Osborne, V. Lanzotti, Plant-derived Natural Products - Synthesis, Function and Application 2009 Springer New York 195 208
4. B. Winkel-Shirley Flavonoid biosynthesis: a colorful model for genetics, biochemistry, cell biology and biotechnology Plant Physiol. 126 2001 485 493 (Pubitemid 32566604)
5. G. Hrazdina, and R.A. Jensen Spatial organization of enzymes in plant metabolic pathways Annu. Rev. Plant Physiol. Plant Mol. Biol. 43 1992 241 267
6. I.E. Burbulis, and B. Winkel-Shirley Interactions among enzymes of the Arabidopsis flavonoid biosynthetic pathway Proc. Natl. Acad. Sci. USA 96 1999 12929 12934 (Pubitemid 29513605)
7. D.K. Owens, A.B. Alerding, K.C. Crosby, A.B. Bandara, J.H. Westwood, and B.S.J. Winkel Functional analysis of a predicted flavonol synthase gene family in Arabidopsis Plant Physiol. 147 2008 1046 1061 (Pubitemid 352844261)
8. D.K. Owens, K.C. Crosby, J. Runac, B.A. Howard, and B.S.J. Winkel Biochemical and genetic characterization of Arabidopsis flavanone 3β-hydroxylase Plant Physiol. Biochem. 46 2008 833 843
9. D. Saslowsky, and B. Winkel-Shirley Localization of flavonoid enzymes in Arabidopsis roots Plant J. 27 2001 37 48 (Pubitemid 32704237)
10. M.K. Pelletier, I.E. Burbulis, and B.W. Shirley Disruption of specific flavonoid genes enhances the accumulation of flavonoid enzymes and endproducts in Arabidopsis seedlings Plant Mol. Biol. 40 1999 45 54
11. G.J. Kremers, J. Goedhart, E.B. van Munster, and T.W.J. Gadella Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Forster radius Biochemistry 45 2006 6570 6580 (Pubitemid 43825357)
12. J. Goedhart, J.E.M. Vermeer, M.J.W. Adjobo-Hermans, L. van Weeren, and T.W.J. Gadella Jr. Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples PLoS ONE 2 2007 e1011
13. M. Karimi, D. Inzé, and A. Depicker GATEWAY((TM)) vectors for Agrobacterium-mediated plant transformation Trends Plant Sci. 7 2002 193 195 (Pubitemid 36725938)
14. S.D. Yoo, Y.H. Cho, and J. Sheen Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis Nat. Protoc. 2 2007 1565 1572 (Pubitemid 47028844)
15. E.B. van Munster, and T.W.J. Gadella FLIM: a new method to avoid aliasing in frequency-domain fluorescence lifetime imaging microscopy J. Microsc.-Oxf. 213 2004 29 38 (Pubitemid 38161149)
16. E.B. van Munster, and T.W.J. Gadella Suppression of photobleaching- induced artifacts in frequency-domain FLIM by permutation of the recording order Cytometry Part A 58A 2004 185 194 (Pubitemid 38509180)
17. N.C. Shaner, R.E. Campbell, P.A. Steinbach, B.N.G. Giepmans, A.E. Palmer, and R.Y. Tsien Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein Nat. Biotechnol. 22 2004 1567 1572 (Pubitemid 40039460)
18. M.J.W. Adjobo-Hermans, J. Goedhart, and T.W.J. Gadella Plant G protein heterotrimers require dual lipidation motifs of G alpha and G gamma and do not dissociate upon activation J. Cell Sci. 119 2006 5087 5097 (Pubitemid 46085187)
19. V. Bayle, L. Nussaume, and R.A. Bhat Combination of novel green fluorescent protein mutant TSapphire and DsRed variant mOrange to set up a versatile in planta FRET-FLIM assay Plant Physiol. 148 2008 51 60 (Pubitemid 352847573)
20. J.E.M. Vermeer, E.B. van Munster, N.O. Vischer, and T.W.J. Gadella Probing plasma membrane microdomains in cowpea protoplasts using lipidated GFP-fusion proteins and multimode FRET microscopy J. Microsc.-Oxf. 214 2004 190 200 (Pubitemid 38553615)
21. P. Broun Transcriptional control of flavonoid biosynthesis: a complex network of conserved regulators involved in multiple aspects of differentiation in Arabidopsis Curr. Opin. Plant Biol. 8 2005 272 279 (Pubitemid 40599981)
22. R. Stracke, H. Ishihara, G.H.A. Barsch, F. Mehrtens, K. Niehaus, and B. Weisshaar Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling Plant J. 50 2007 660 677 (Pubitemid 46733755)
23. R. Stracke, R.C.H. De Vos, L. Bartelniewoehner, H. Ishihara, M. Sagasser, S. Martens, and B. Weisshaar Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase Planta 229 2009 427 445
24. N. Trabelsi, P. Petit, C. Manigand, B.L. d'Estaintot, T. Granier, J. Chaudiere, and B. Gallois Structural evidence for the inhibition of grape dihydroflavonol 4-reductase by flavonols Acta Crystallogr. Sect. D: Biol. Crystallogr. 64 2008 883 891 (Pubitemid 352032292)
25. M. Leibundgut, T. Maier, S. Jenni, and N. Ban The multienzyme architecture of eukaryotic fatty acid synthases Curr. Opin. Struct. Biol. 18 2008 714 725
26. C.Q. Mo, and M. Bard A systematic study of yeast sterol biosynthetic protein-protein interactions using the split-ubiquitin system Biochim. Biophys. Acta, Mol. Cell Biol. Lipids 1737 2005 152 160 (Pubitemid 41740710)
27. J.J. Turnbull, W.J. Sobey, R.T. Aplin, A. Hassan, J.L. Firmin, C.J. Schofield, and A.G. Prescott Are anthocyanidins the immediate precursors of anthocyanidin synthase? Chem. Commun. 24 2000 2473 2474 (Pubitemid 32001355)
28. J.P. Noel, M.B. Austin, and E.K. Bomati Structure-function relationships in plant phenylpropanoid biosynthesis Curr. Opin. Plant Biol. 8 2005 249 253 (Pubitemid 40599979)
29. H.A. Stafford Flavonoid evolution - an enzymatic approach Plant Physiol. 96 1991 680 685
30. P. Dutow, S.R. Schmidl, M. Ridderbusch, and J. Stülke Interactions between glycolytic enzymes of Mycoplasma pneumoniae J. Mol. Microbiol. Biotechnol. 19 2010 134 139
31. P.A. Srere Macromolecular interactions: tracing the roots Trends Biochem. Sci. 25 2000 150 153 (Pubitemid 30122429)
32. J. Ovádi, and V. Saks On the origin of intracellular compartmentation and organized metabolic systems Mol. Cell. Biochem. 256-257 2004 5 12 (Pubitemid 38181231)
33. B. Winkel Metabolic channeling in plants Annu. Rev. Plant Biol. 55 2004 85 107 (Pubitemid 38940927)