Using the Q system in Drosophila melanogaster

Nature Protocols

Volumn 6, Issue 8, 2011, Pages 1105-1120

  Potter, Christopher J ^a   Luo, Liqun ^b  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSCRIPTION FACTOR GAL4;

ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; CATABOLISM; DROSOPHILA MELANOGASTER; GENE EXPRESSION; GENE EXPRESSION SYSTEM; IN VIVO STUDY; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; Q SYSTEM; TRANSGENE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CLONING, MOLECULAR; DROSOPHILA MELANOGASTER; GENE EXPRESSION; GENETIC ENGINEERING; NEUROSPORA CRASSA; QUINIC ACID; TRANSGENES;

DROSOPHILA MELANOGASTER;

EID: 79960260970     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2011.347     Document Type: Article

References (54)

1. Brand, A.H. & Perrimon, N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118, 401-415 (1993). (Pubitemid 23214028)
2. Duffy, J.B. GAL4 system in Drosophila: a fly geneticists Swiss army knife. Genesis 34, 1-15 (2002).
3. Lee, T. & Luo, L. Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron 22, 451-461 (1999). (Pubitemid 29159845)
4. Potter, C.J., Tasic, B., Russler, E.V., Liang, L. & Luo, L. The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis. Cell 141, 536-548 (2010).
5. Baum, J.A., Geever, R. & Giles, N.H. Expression of qa-1F activator protein: identification of upstream binding sites in the qa gene cluster and localization of the DNA-binding domain. Mol. Cell Biol. 7, 1256-1266 (1987). (Pubitemid 17017449)
6. Geever, R.F. et al. DNA sequence, organization and regulation of the qa gene cluster of Neurospora crassa. J. Mol. Biol. 207, 15-34 (1989). (Pubitemid 19141239)
7. Giles, N.H., Geever, R.F., Asch, D.K., Avalos, J. & Case, M.E. The Wilhelmine E. Key 1989 invitational lecture. Organization and regulation of the qa (quinic acid) genes in Neurospora crassa and other fungi. J. Hered. 82, 1-7 (1991).
8. Huiet, L. Molecular analysis of the Neurospora qa-1 regulatory region indicates that two interacting genes control qa gene expression. Proc. Natl. Acad. Sci. USA 81, 1174-1178 (1984). (Pubitemid 14168242)
9. Patel, V.B. & Giles, N.H. Autogenous regulation of the positive regulatory qa-1F gene in Neurospora crassa. Mol. Cell Biol. 5, 3593-3599 (1985). (Pubitemid 16233066)
10. Wu, J.S. & Luo, L. A protocol for mosaic analysis with a repressible cell marker (MARCM) in Drosophila. Nat. Protoc. 1, 2583-2589 (2006).
11. Berdnik, D., Fan, A.P., Potter, C.J. & Luo, L. MicroRNA processing pathway regulates olfactory neuron morphogenesis. Curr. Biol. 18, 1754-1759 (2008).
12. Jefferis, G.S.X.E. et al. Comprehensive maps of Drosophila higher olfactory centers: spatially segregated fruit and pheromone representation. Cell 128, 1187-1203 (2007). (Pubitemid 46427875)
13. Schuldiner, O. et al. piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning. Dev. Cell 14, 227-238 (2008). (Pubitemid 351189171)
14. Lai, S.-L. & Lee, T. Genetic mosaic with dual binary transcriptional systems in Drosophila. Nat. Neurosci. 9, 703-709 (2006). (Pubitemid 44012270)
15. Yagi, R., Mayer, F. & Basler, K. Refined LexA transactivators and their use in combination with the Drosophila Gal4 system. Proc. Natl Acad. Sci. USA 107, 16166-16171 (2010).
16. Pfeiffer, B.D. et al. Refinement of tools for targeted gene expression in Drosophila. Genetics 186, 735-755 (2010).
17. Suster, M.L., Seugnet, L., Bate, M. & Sokolowski, M.B. Refining GAL4-driven transgene expression in Drosophila with a GAL80 enhancer-trap. Genesis 39, 240-245 (2004). (Pubitemid 39201545)
18. Luan, H., Peabody, N.C., Vinson, C.R. & White, B.H. Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. Neuron 52, 425-436 (2006). (Pubitemid 44667428)
19. Lai, S.-L., Awasaki, T., Ito, K. & Lee, T. Clonal analysis of Drosophila antennal lobe neurons: diverse neuronal architectures in the lateral neuroblast lineage. Development 135, 2883-2893 (2008).
20. Yu, H., Chen, C., Shi, L., Huang, Y. & Lee, T. Twin-spot MARCM to reveal the developmental origin and identity of neurons. Nat. Neurosci. 12, 947-953 (2009).
21. Yu, H.-H. et al. A complete developmental sequence of a Drosophila neuronal lineage as revealed by Twin-Spot MARCM. PLoS Biol. 8, (2010).
22. Griffin, R. et al. The twin spot generator for differential Drosophila lineage analysis. Nat. Methods 6, 600-602 (2009).
23. Zong, H., Espinosa, J.S., Su, H.H., Muzumdar, M.D. & Luo, L. Mosaic analysis with double markers in mice. Cell 121, 479-492 (2005). (Pubitemid 40692306)
24. Fishilevich, E. et al. Chemotaxis behavior mediated by single larval olfactory neurons in Drosophila. Curr. Biol. 15, 2086-2096 (2005). (Pubitemid 41739740)
25. Ang, L.-H. et al. Lim kinase regulates the development of olfactory and neuromuscular synapses. Dev. Biol. 293, 178-190 (2006). (Pubitemid 44316548)
26. Pfeiffer, B.D. et al. Tools for neuroanatomy and neurogenetics in Drosophila. Proc. Natl. Acad. Sci. USA 105, 9715-9720 (2008). (Pubitemid 352031366)
27. Venken, K. et al. Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster. Nat. Methods 6, 431-434 (2009).
28. Markstein, M., Pitsouli, C., Villalta, C., Celniker, S.E. & Perrimon, N. Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes. Nat. Genet. 40, 476-483 (2008). (Pubitemid 351450875)
29. Groth, A.C., Fish, M., Nusse, R. & Calos, M.P. Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Genetics 166, 1775-1782 (2004). (Pubitemid 38621059)
30. Sullivan, W., Ashburner, M. & Scott Hawley, R. Drosophila Protocols. Cold Spring Harbor Laboratory Press (2000).
31. Wu, J.S. & Luo, L. A protocol for dissecting Drosophila melanogaster brains for live imaging or immunostaining. Nat. Protoc. 1, 2110-2115 (2006). (Pubitemid 46773334)
32. Watts, R.J., Schuldiner, O., Perrino, J., Larsen, C. & Luo, L. Glia engulf degenerating axons during developmental axon pruning. Curr. Biol. 14, 678-684 (2004). (Pubitemid 38503604)
33. LaJeunesse, D.R. et al. Three new Drosophila markers of intracellular membranes. BioTechniques 36, 784-788, 790 (2004).
34. Nicolai, L.J. et al. Genetically encoded dendritic marker sheds light on neuronal connectivity in Drosophila. Proc. Natl. Acad. Sci. USA 107, 20553-20558 (2010).
35. Grieder, N.C., de Cuevas, M. & Spradling, A.C. The fusome organizes the microtubule network during oocyte differentiation in Drosophila. Development 127, 4253-4264 (2000).
36. Potter, C.J., Huang, H. & Xu, T. Drosophila Tsc1 functions with Tsc2 to antagonize insulin signaling in regulating cell growth, cell proliferation, and organ size. Cell 105, 357-368 (2001). (Pubitemid 32455342)
37. Potter, C.J., Pedraza, L.G. & Xu, T. Akt regulates growth by directly phosphorylating Tsc2. Nat. Cell Biol. 4, 658-665 (2002). (Pubitemid 34993701)
38. Zhou, C., Rao, Y. & Rao, Y. A subset of octopmanergic neurons play important roles in Drosophila aggression. Nat. Neurosci. 11, 1059-1067 (2008).
39. White, K. et al. Genetic control of programmed cell death in Drosophila. Science 264, 677-683 (1994). (Pubitemid 24185877)
40. Grether, M.E., Abrams, J.M., Agapite, J., White, K. & Steller, H. The head involution defective gene of Drosophila melanogaster functions in programmed cell death. Genes Dev. 9, 1694-1708 (1995).
41. Chen, P., Nordstrom, W., Gish, B. & Abrams, J.M. grim, a novel cell death gene in Drosophila. Genes Dev. 10, 1773-1782 (1996). (Pubitemid 26272383)
42. Seelig, J.D. et al. Two-photon calcium imaging from head-fixed Drosophila during optomotor walking behavior. Nat. Methods 7, 535-540 (2010).
43. Prober, D.A. & Edgar, B.A. Interactions between Ras1, dMyc, and dPI3K signaling in the developing Drosophila wing. Genes Dev. 16, 2286-2299 (2002). (Pubitemid 35013091)
44. Dietzl, G. et al. A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448, 151-156 (2007). (Pubitemid 47067374)
45. Shi, L., Yu, H.H., Yang, J.S. & Lee, T. Specific Drosophila Dscam juxtamembrane variants control dendritic elaboration and axonal arborization. J. Neurosci. 27, 6723-6728 (2007). (Pubitemid 46983422)
46. Boyden, E.S., Zhang, F., Bamberg, E., Nagel, G. & Deisseroth, K. Millisecond-timescale, genetically targeted optical control of neural activity. Nat. Neurosci. 8, 1263-1268 (2005). (Pubitemid 43348147)
47. Zhang, W., Ge, W. & Wang, Z. A toolbox for light control of Drosophila behaviors through Channelrhodopsin 2-mediated photoactivation of targeted neurons. Eur. J. Neurosci. 26, 2405-2416 (2007). (Pubitemid 350050436)
48. Peabody, N.C. et al. Characterization of the decision network for wing expansion in Drosophila using targeted expression of the TRPM8 channel. J. Neurosci. 29, 3343-3353 (2009).
49. Hamada, F.N. et al. An internal thermal sensor controlling temperature preference in Drosophila. Nature 454, 217-220 (2008). (Pubitemid 351969888)
50. Kitamoto, T. Conditional modification of behavior in Drosophila by targeted expression of a temperature-sensitive shibire allele in defined neurons. J. Neurobiol. 47, 81-92 (2001).
51. Paradis, S., Sweeney, S.T. & Davis, G.W. Homeostatic control of presynaptic release is triggered by postsynaptic membrane depolarization. Neuron 30, 737-749 (2001). (Pubitemid 32607338)
52. Sweeney, S.T., Broadie, K., Keane, J., Niemann, H. & OKane, C.J. Targeted expression of tetanus toxin light chain in Drosophila specifically eliminates synaptic transmission and causes behavioral defects. Neuron 14, 341-351 (1995).
53. Suster, M.L., Martin, J.-R., Sung, C. & Robinow, S. Targeted expression of tetanus toxin reveals sets of neurons involved in larval locomotion in Drosophila. J. Neurobiol. 55, 233-246 (2003). (Pubitemid 36423161)
54. Xu, T. & Rubin, G.M. Analysis of genetic mosaics in developing and adult Drosophila tissues. Development 117, 1223-1237 (1993). (Pubitemid 23154489)