Effect of ploidy increase on transgene expression: Example from Citrus diploid cybrid and allotetraploid somatic hybrid expressing the EGFP gene

Protoplasma

Volumn 248, Issue 3, 2011, Pages 531-540

  Xu, Shi Xiao ^a   Cai, Xiao Dong ^a   Tan, Bin ^a   Li, Ding Li ^a   Guo, Wen Wu ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Citrus; Confocal laser scanning microscopy; Green fluorescent protein; Ploidy level; Somatic hybridization

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN;

ARTICLE; BIOSYNTHESIS; CITRUS; DIPLOIDY; GENE EXPRESSION; GENETICS; HYBRIDIZATION; SWEET ORANGE; TETRAPLOIDY; TRANSGENE; TRANSGENIC PLANT;

CITRUS; CITRUS SINENSIS; DIPLOIDY; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; HYBRIDIZATION, GENETIC; PLANTS, GENETICALLY MODIFIED; TETRAPLOIDY; TRANSGENES;

CITRUS; CITRUS NOBILIS; CITRUS RETICULATA; CITRUS SINENSIS; EUKARYOTA;

EID: 79960241648     PISSN: 0033183X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00709-010-0200-2     Document Type: Article

References (46)

1. Ainouche ML, Jenczewski E (2010) Focus on polyploidy. New Phytol 186: 1-4.
2. Albertin W, Balliau T, Brabant P, Chevre A, Eber F, Malosse C, Thiellement H (2006) Numerous and rapid nonstochastic modifications of gene products in newly synthesized Brassica napus allotetraploids. Genetics 173: 1101-1113.
3. Al-Janabi SM, McClelland M, Petersen C, Sobral BWS (1994) Phylogenetic analysis of organellar DNA sequences in the Andropogoneae: Saccharinea. Theor Appl Genet 88: 933-944.
4. Banaei Moghaddam AM, Fuchs J, Czauderna T, Houben A, Mette MF (2010) Intraspecific hybrids of Arabidopsis thaliana revealed no gross alterations in endopolyploidy, DNA methylation, histone modifications and transcript levels. Theor Appl Genet 120: 215-226.
5. Beaujean A, Sangwan RS, Hodges M, Sangwan-Norreel BS (1998) Effect of ploidy and homozygosity on transgene expression in primary tobacco transformants and their androgenetic progenies. Mol Gen Genet 260: 362-371.
6. Beaulieu J, Jean M, Belzile F (2009) The allotetraploid Arabidopsis thaliana-Arabidopsis lyrata subsp petraea as an alternative model system for the study of polyploidy in plants. Mol Genet Genomics 281: 421-435.
7. Bottley A, Xia G, Koebner RMD (2006) Homoeologous gene silencing in hexaploid wheat. Plant J 47: 897-906.
8. Bryan GJ, McNicoll J, Ramsay G, Meyer RC, De Jong WS (1999) Polymorphic simple sequence repeat markers in chloroplast genomes of Solanaceous plants. Theor Appl Genet 99: 859-867.
9. Cai XD, Liu X, Guo WW (2006) GFP expression as an indicator of somatic hybrids between transgenic Satsuma mandarin and calamondin at embryoid stage. Plant Cell Tissue Organ Cult 87: 245-253.
10. Cai XD, Fu J, Deng XX, Guo WW (2007) Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma mandarin and two seedy Citrus cultivars. Plant Cell Tissue Organ Cult 90: 275-283.
11. Chen ZJ (2007) Genetic and epigenetic mechanisms for gene expression and phenotypic variation in plant polyploids. Annu Rev Plant Biol 58: 377-406.
12. Cheng YJ, Guo WW, Yi HL, Pang XM, Deng XX (2003a) An efficient protocol for genomic DNA extraction from Citrus species. Plant Mol Biol Reporter 21: 177a-177g.
13. Cheng YJ, Guo WW, Deng XX (2003b) Molecular characterization of cytoplasmic and nuclear genomes in phenotypically abnormal Valencia orange (Citrus sinensis) + Meiwa kumquat (Fortunella crassifolia) intergeneric somatic hybrids. Plant Cell Rep 21: 445-451.
14. Davey MR, Anthony P, Power JB, Lowe KC (2005) Plant protoplasts: status and biotechnological perspectives. Biotechnol Adv 23: 131-171.
15. Gaeta RT, Yoo SY, Pires JC, Doerge RW, Chen ZJ, Osborn TC (2009) Analysis of gene expression in resynthesized Brassica napus allopolyploids using Arabidopsis 70mer oligo microarrays. PLoS ONE 4(3): e4760.
16. Giovanni C, Maurizio B, Luca M, Laura S, Gian Marco G, Barbara C, Paola O, Luciano Z, Pier GR (2004) Blue silver: a very sensitive colloidal coomassie G-250 staining for proteome analysis. Electrophoresis 25: 1327-1333.
17. Grosser JW, Gmitter FG Jr (1990) Protoplast fusion and Citrus improvement. Plant Breed Rev 8: 339-374.
18. Guo WW, Deng XX (1998) Somatic hybrid plantlets regeneration between Citrus and its wild relative, Murraya paniculata via protoplast electrofusion. Plant Cell Rep 18: 297-300.
19. Guo WW, Grosser JW (2005) Somatic hybrid vigor in Citrus: direct evidence from protoplast fusion of an embryogenic callus line with a transgenic mesophyll parent expressing the GFP gene. Plant Sci 168: 1541-1545.
20. Guo WW, Prasad D, Cheng YJ, Serrano P, Deng XX, Grosser JW (2004) Targeted cybridization in citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness. Plant Cell Rep 22: 752-758.
21. Guo WW, Duan YX, Olivares-Fuster O, Wu ZC, Arias CR, Burns JK, Grosser JW (2005) Protoplast transformation and regeneration of transgenic Valencia sweet orange plants containing a juice quality-related pectin methylesterase gene. Plant Cell Rep 24: 482-486.
22. Ha M, Lu J, Tian L, Ramachandran V, Kasschau KD, Chapman EJ, Carrington JC, Chen XM, Wang XJ, Chen ZJ (2009) Small RNAs serve as a genetic buffer against genomic shock in Arabidopsis interspecific hybrids and allopolyploids. Proc Natl Acad Sci 106: 17835-17840.
23. Haseloff J, Siemering KR (2006) The uses of green fluorescent protein in plants. Methods Biochem Anal 47: 259-284.
24. Hraska M, Rakousky S, Curn V (2006) Green fluorescent protein as a vital marker for non-destructive detection of transformation events in transgenic plants. Plant Cell Tissue Organ Cult 86: 303-318.
25. Hraska M, Hermanova V, Rakousky S, Curn V (2008) Sample topography and position within plant body influence the detection of the intensity of green fluorescent protein fluorescence in the leaves of transgenic tobacco plants. Plant Cell Rep 27: 67-77.
26. Isaacson T, Damasceno CMB, Saravanan RS, He Y, Catala C, Saladie M, Rose JKC (2006) Sample extraction techniques for enhanced proteomic analysis of plant tissues. Nat Protoc 1: 769-774.
27. Johnson AAT, Nault BA, Veilleux RE (2003) Transmission of a Bacillus thuringiensis cry3Aa transgene from diploid to tetraploid potato using 4x-2x hybridization: effect of ploidy increase on transgene expression and implications for TPS hybrid production. Plant Breed 122: 223-228.
28. Kijas JMH, Thomas MR, Fowler JCS, Roose ML (1997) Integration of trinucleotide microsatellite into a linkage map of Citrus. Theor Appl Genet 94: 701-708.
29. Lee JM, Roche JR, Donaghy DJ, Thrush A, Sathish P (2010) Validation of reference genes for quantitative RT-PCR studies of gene expression in perennial ryegrass (Lolium perenne L.). BMC Mol Biol 11: 8.
30. Liu Q, Xu J, Liu YZ, Zhao XL, Deng XX, Guo LL, Gu JQ (2007) A novel bud mutation that confers abnormal patterns of lycopene accumulation in sweet orange fruit (Citrus sinensis L. Osbeck). J Exp Bot 58: 4161-4171.
31. Mittelsten Scheid O, Jakovleva L, Afsar K, Maluszynska J, Paszkowski J (1996) A change of ploidy can modify epigenetic silencing. Proc Natl Acad Sci 93: 7114-7119.
32. Murashige T, Tucker DPH (1969) Growth factor requirements of Citrus tissue culture. In: Chapman HD (ed) Proc 1st Intl Citrus Symp, vol 3. University of California Riverside, Riverside, pp 1155-1161.
33. Ochogavia AC, Cervigni G, Selva JP, Echenique VC, Pessino SC (2009) Variation in cytosine methylation patterns during ploidy level conversions in Eragrostis curvula. Plant Mol Biol 70: 17-29.
34. Olivares-Fuster O, Pena L, Duran-Vila N, Navarro L (2002) Green fluorescent protein as a visual marker in somatic hybridization. Ann Bot 89: 491-497.
35. Pignatta D, Dilkes B, Wroblewski T, Michelmore RW, Comai L (2008) Transgene-induced gene silencing is not affected by a change in ploidy level. PLoS ONE 3(8): e3061.
36. Riddle NC, Jiang HM, An LL, Doerge RW, Birchler JA (2010) Gene expression analysis at the intersection of ploidy and hybridity in maize. Theor Appl Genet 120: 341-353.
37. Robic G, Lacorte C, Miranda EA (2009) Fluorometric quantification of green fluorescent protein in tobacco leaf extracts. Anal Biochem 392: 8-11.
38. Salmon A, Ainouche ML (2010) Polyploidy and DNA methylation: new tools available. Mol Ecol 19: 213-215.
39. Southern E (2006) Southern blotting. Nat Protocol 1: 518-525.
40. Towbin H, Gordon TS (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci 76: 4350-4354.
41. van de Peer Y, Maere S, Meyer A (2009) Opinion the evolutionary significance of ancient genome duplications. Nat Rev Genet 10: 725-732.
42. Verhoeven KJF, van Dijk PJ, Biere A (2010) Changes in genomic methylation patterns during the formation of triploid asexual dandelion lineages. Mol Ecol 19: 315-324.
43. Wan Y, Poole RL, Huttly AK, Toscano-Underwood C, Feeney K, Welham S, Gooding MJ, Mills C, Edwards KJ, Shewry PR, Mitchell RAC (2008) Transcriptome analysis of grain development in hexaploid wheat. BMC Genomics 9: 121.
44. Wang JL, Tian L, Lee H, Wei NE, Jiang HM, Watson B, Madlung A, Osborn TC, Doerge RW, Comai L, Chen ZJ (2006) Genome-wide nonadditive gene regulation in Arabidopsis allotetraploids. Genetics 172: 507-517.
45. Xu YH, Zhong L, Wu XM, Fang XP, Wang JB (2009) Rapid alterations of gene expression and cytosine methylation in newly synthesized Brassica napus allopolyploids. Planta 229: 471-483.
46. Zhou X, Carranco R, Vitha S, Hall TC (2005) The dark side of green fluorescent protein. New Phytol 168: 313-321.