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Volumn 7, Issue 5, 2011, Pages 618-628

Expression, purification and characterization of a novel soluble human thymosin alpha1 concatemer exhibited a stronger stimulation on mice lymphocytes proliferation and higher anti-tumor activity

Author keywords

Concatemer; Protein expression; Purification; Thymosin alpha 1; Tumor suppression

Indexed keywords

ESCHERICHIA COLI; MUS;

EID: 79960136040     PISSN: 14492288     EISSN: None     Source Type: Journal    
DOI: 10.7150/ijbs.7.618     Document Type: Article
Times cited : (8)

References (36)
  • 1
    • 0017334767 scopus 로고
    • Thymosin alpha l: Isolation and sequence analysis of an immunologically active thymic polypeptide
    • Goldstein Al, Low TL, McAdoo M, et al. Thymosin alpha l: isolation and sequence analysis of an immunologically active thymic polypeptide. Proc. Natl. Acad. Sci. 1977; 74(2):725-729.
    • (1977) Proc. Natl. Acad. Sci , vol.74 , Issue.2 , pp. 725-729
    • Goldstein, A.1    Low, T.L.2    McAdoo, M.3
  • 4
    • 1842734249 scopus 로고    scopus 로고
    • Thymosins: Chemistry and bio-logical properties in health and disease
    • Goldstein AL, Badamchian M. Thymosins: chemistry and bio-logical properties in health and disease. Expert Opin Biol Ther. 2004; 4(4):559.
    • (2004) Expert Opin Biol Ther , vol.4 , Issue.4 , pp. 559
    • Goldstein, A.L.1    Badamchian, M.2
  • 5
    • 20044372562 scopus 로고    scopus 로고
    • Roles of thymosins in cancers and other organ systems
    • Chen C, Li M, Yang H, et al. Roles of thymosins in cancers and other organ systems. World J. Surg. 2005; 29(3): 264-270.
    • (2005) World J. Surg , vol.29 , Issue.3 , pp. 264-270
    • Chen, C.1    Li, M.2    Yang, H.3
  • 6
    • 0030949228 scopus 로고    scopus 로고
    • Thymosin α1 an-tagonizes dexamethasone and CD3-induced apoptosis of CD4+CD8+ thymocytes through the activation of cAMP and protein kinase C dependent second messenger pathways
    • Baumann CA, Badamchian M, Golstein AL. Thymosin α1 an-tagonizes dexamethasone and CD3-induced apoptosis of CD4+CD8+ thymocytes through the activation of cAMP and protein kinase C dependent second messenger pathways. Mechanism of Ageing and Development. 1997; 94:85-101.
    • (1997) Mechanism of Ageing and Development , vol.94 , pp. 85-101
    • Baumann, C.A.1    Badamchian, M.2    Golstein, A.L.3
  • 7
    • 0033952756 scopus 로고    scopus 로고
    • Mechanism of thymocyte apop-tosis induced by serum of tumor-bearing host: The molecular events involved and their inhibition by thymosin α-1
    • Roy R, Singh SM, Shanker A. Mechanism of thymocyte apop-tosis induced by serum of tumor-bearing host: The molecular events involved and their inhibition by thymosin α-1. Int. J. Immunopharmacol. 2000; 22: 309-321.
    • (2000) Int. J. Immunopharmacol , vol.22 , pp. 309-321
    • Roy, R.1    Singh, S.M.2    Shanker, A.3
  • 8
    • 0024418051 scopus 로고
    • Interleukin 2 receptor expression in patients with head and neck squamous carcino-ma.Effects of thymosin alpha 1 in vitro
    • Wolf GT, Hudson J, Peterson KA, et al. Interleukin 2 receptor expression in patients with head and neck squamous carcino-ma.Effects of thymosin alpha 1 in vitro. Arch. Otolaryngol Head Neck Surg. 1989;115:1345-9.
    • (1989) Arch. Otolaryngol Head Neck Surg , vol.115 , pp. 1345-1349
    • Wolf, G.T.1    Hudson, J.2    Peterson, K.A.3
  • 9
    • 33746909124 scopus 로고    scopus 로고
    • Thymosin alpha1 sup-presses proliferation and induces apoptosis in human leukemia cell lines
    • Ying-zhe Fan, Hui Chang, Ye Yu, et al. Thymosin alpha1 sup-presses proliferation and induces apoptosis in human leukemia cell lines. Peptides. 2006;27(9): 2165-2173.
    • (2006) Peptides , vol.27 , Issue.9 , pp. 2165-2173
    • Ying-Zhe, F.1    Hui, C.2    Ye, Y.3
  • 10
    • 19444385803 scopus 로고    scopus 로고
    • Presence of a peptide component of thymo-sin fraction-5 manifesting discrete cytostatic properties in HL-60 human promyelocytic leukemia cells
    • Spangelo BL, et al. Presence of a peptide component of thymo-sin fraction-5 manifesting discrete cytostatic properties in HL-60 human promyelocytic leukemia cells. International Im-munopharmacology. 2005; 5(7-8): 1317-1329.
    • (2005) International Im-munopharmacology , vol.5 , Issue.7-8 , pp. 1317-1329
    • Spangelo, B.L.1
  • 11
    • 0027420351 scopus 로고
    • Thymosin alpha 1 down-regulates the growth of human non-small cell lung can-cer cells in vitro and in vivo
    • Moody TW, Fagarasan M, Zia F, et al. Thymosin alpha 1 down-regulates the growth of human non-small cell lung can-cer cells in vitro and in vivo. Cancer Res. 1993; 53: 5214-8.
    • (1993) Cancer Res , vol.53 , pp. 5214-5218
    • Moody, T.W.1    Fagarasan, M.2    Zia, F.3
  • 13
    • 0018394926 scopus 로고
    • The chemistry and biology of thymosin
    • Low TL, et al. The chemistry and biology of thymosin. J. Biol. Chem. 1979;254:981-986.
    • (1979) J. Biol. Chem , vol.254 , pp. 981-986
    • Low, T.L.1
  • 14
    • 0021207068 scopus 로고
    • Thymosins: Structure, function and therapeutic applications
    • Low TL, Goldstein AL. Thymosins: structure, function and therapeutic applications. Thymus. 1984; 6: 27-42.
    • (1984) Thymus , vol.6 , pp. 27-42
    • Low, T.L.1    Goldstein, A.L.2
  • 15
    • 0035179789 scopus 로고    scopus 로고
    • Thymosin alpha 1 accelerates restoration of T-cell-mediated neutralizing antibody response in immunocompromised hosts
    • Li CL, Zhang T, Saibara T, et al. Thymosin alpha 1 accelerates restoration of T-cell-mediated neutralizing antibody response in immunocompromised hosts. Int. Immunopharma-col.2002;2:39-46.
    • (2002) Int. Immunopharma-col , vol.2 , pp. 39-46
    • Li, C.L.1    Zhang, T.2    Saibara, T.3
  • 16
    • 0038307784 scopus 로고    scopus 로고
    • Immunodeficiency and cancer: Prospects for cor-rection
    • Hadden JW. Immunodeficiency and cancer: prospects for cor-rection. Int Immunopharmacol.2003;3:1061-71.
    • (2003) Int Immunopharmacol , vol.3 , pp. 1061-1071
    • Hadden, J.W.1
  • 17
    • 0034535749 scopus 로고    scopus 로고
    • Thymosin alpha 1 in the treatment of cancer: From basic research to clinical application
    • Garaci E, Pica F, Rasi G, et al. Thymosin alpha 1 in the treatment of cancer: from basic research to clinical application. Int. J. Immunopharmacol. 2000; 22: 1067-76.
    • (2000) Int. J. Immunopharmacol , vol.22 , pp. 1067-1076
    • Garaci, E.1    Pica, F.2    Rasi, G.3
  • 18
    • 0036104753 scopus 로고    scopus 로고
    • Treatment of chronic hepatitis B: Case selection and duration of therapy
    • Leung N. Treatment of chronic hepatitis B: case selection and duration of therapy. Gastroenterol Hepatol. 2002;17:409-14.
    • (2002) Gastroenterol Hepatol , vol.17 , pp. 409-414
    • Leung, N.1
  • 19
    • 0348012900 scopus 로고    scopus 로고
    • A pilot study of the safety and efficacy of thymosin alpha 1 in augmentingimmune reconstitution in HIV-infected patients with low CD4 counts taking highly active antiretroviral therapy
    • Chadwick D, Pido-Lopez J, Pires A, et al. A pilot study of the safety and efficacy of thymosin alpha 1 in augmentingimmune reconstitution in HIV-infected patients with low CD4 counts taking highly active antiretroviral therapy. Clin Exp Immunol. 2003;134:477-81.
    • (2003) Clin Exp Immunol , vol.134 , pp. 477-481
    • Chadwick, D.1    Pido-Lopez, J.2    Pires, A.3
  • 20
    • 0037816287 scopus 로고    scopus 로고
    • Thymosin alpha(1) in combination with cytokines and chemotherapy for the treatment of cancer
    • Garaci E, Pica F, Sinibaldi-Vallebona P, et al. Thymosin alpha(1) in combination with cytokines and chemotherapy for the treatment of cancer. Int Immunopharmacol. 2003;3:1145-50.
    • (2003) Int Immunopharmacol , vol.3 , pp. 1145-1150
    • Garaci, E.1    Pica, F.2    Sinibaldi-Vallebona, P.3
  • 21
    • 0036557949 scopus 로고    scopus 로고
    • Thymosin α1(SciClone Pharmaceuticals) Current Opioion in Investigational
    • Billich A. Thymosin α1(SciClone Pharmaceuticals) Current Opioion in Investigational. Drugs. 2002;3: 698-707.
    • (2002) Drugs , vol.3 , pp. 698-707
    • Billich, A.1
  • 22
    • 0031762984 scopus 로고    scopus 로고
    • High doses of thymosin alpha 1 enhance the anti-tumor efficacy of combination chemo-immunotherapy for murine B16 melanoma
    • Pica F, Fraschetti M, Matteucci C, et al. High doses of thymosin alpha 1 enhance the anti-tumor efficacy of combination chemo-immunotherapy for murine B16 melanoma. Anticancer Res. 1998;18:3571-3578.
    • (1998) Anticancer Res , vol.18 , pp. 3571-3578
    • Pica, F.1    Fraschetti, M.2    Matteucci, C.3
  • 23
    • 0036078943 scopus 로고    scopus 로고
    • Thymosin alpha1 inhibits mammary carcinogenesis in Fisher rats
    • Moody TW, Tuthill C, Badamchian M, et al. Thymosin alpha1 inhibits mammary carcinogenesis in Fisher rats. Peptides. 2002;23: 1011-1014.
    • (2002) Peptides , vol.23 , pp. 1011-1014
    • Moody, T.W.1    Tuthill, C.2    Badamchian, M.3
  • 24
    • 0038342434 scopus 로고    scopus 로고
    • Combination therapy in the treatment of chronic viral hepatitis and preven-tion of hepatocellular carcinoma
    • Rasi G, Pierimarchi P, Sinibaldi Vallebona P, et al. Combination therapy in the treatment of chronic viral hepatitis and preven-tion of hepatocellular carcinoma. Int. Immunopharmacol. 2003; 3: 1169-1176.
    • (2003) Int. Immunopharmacol , vol.3 , pp. 1169-1176
    • Rasi, G.1    Pierimarchi, P.2    Vallebona, P.S.3
  • 25
    • 28744446178 scopus 로고    scopus 로고
    • Primary assessment of treatment effect of thymosin alpha1 on chemotherapy-induced neurotoxicity
    • An TT, Liu XY, Fang J. Primary assessment of treatment effect of thymosin alpha1 on chemotherapy-induced neurotoxicity. Ai Zheng. 2004;23(11): 1428-30.
    • (2004) Ai Zheng , vol.23 , Issue.11 , pp. 1428-1430
    • An, T.T.1    Liu, X.Y.2    Fang, J.3
  • 26
    • 17644364797 scopus 로고    scopus 로고
    • Construction and expression of a new fusion protein, thymosin a1-cBLyS in E.coli
    • Qiong Shen, Ruiyang Tian, Wenzhe Ma. Construction and expression of a new fusion protein, thymosin a1-cBLyS in E.coli. Biotechnology Letters. 2005; 27: 143-148.
    • (2005) Biotechnology Letters , vol.27 , pp. 143-148
    • Qiong, S.1    Ruiyang, T.2    Wenzhe, M.3
  • 27
    • 48749133790 scopus 로고    scopus 로고
    • Expression and Hydroxylamine Cleavage of Thymosin Alpha 1 Concatemer
    • Zhou L, Lai ZT, Lu MK, et al. Expression and Hydroxylamine Cleavage of Thymosin Alpha 1 Concatemer. Journal of Bio-medicine and Biotechnology. 2008;2008:736060.
    • (2008) Journal of Bio-medicine and Biotechnology , vol.2008 , pp. 736060
    • Zhou, L.1    Lai, Z.T.2    Lu, M.K.3
  • 28
    • 18944399035 scopus 로고    scopus 로고
    • Construction, expression and characterization of human interferon α2b-(G4S)n-thymosin α1 fusion proteins in Pichia pastoris
    • You-Feng Yang, Han-Ying Yuan, Nan-Song Liu. Construction, expression and characterization of human interferon α2b-(G4S)n-thymosin α1 fusion proteins in Pichia pastoris. World J. Gastroenterol. 2005;11:2597-2602.
    • (2005) World J. Gastroenterol , vol.11 , pp. 2597-2602
    • You-Feng, Y.1    Han-Ying, Y.2    Nan-Song, L.3
  • 30
    • 38349047619 scopus 로고    scopus 로고
    • Expression and analysis of thy-mosin α1 concatemer in Escherichia coli
    • Chen Y, Zhao L, Shen G, et al. Expression and analysis of thy-mosin α1 concatemer in Escherichia coli. Biotechnol. Appl. Bi-ochem. 2008;49:51-56.
    • (2008) Biotechnol. Appl. Bi-ochem , vol.49 , pp. 51-56
    • Chen, Y.1    Zhao, L.2    Shen, G.3
  • 31
    • 67349228394 scopus 로고    scopus 로고
    • Proliferative and anti-proliferative effects of thymosin α1 on cells are associated with manipulation of cellular ROS levels
    • Yong Qin, Fu-Ding Chen, Liang Zhou, et al. Proliferative and anti-proliferative effects of thymosin α1 on cells are associated with manipulation of cellular ROS levels. J. Chemico-biological Interactions. 2009;180:383-8.
    • (2009) J. Chemico-biological Interactions , vol.180 , pp. 383-388
    • Yong, Q.1    Fu-Ding, C.2    Liang, Z.3
  • 32
    • 0020643124 scopus 로고
    • Isolation of thymosin alpha 1 from thymosin fraction 5 of different species by high-performance liquid chromatography
    • Low TL, McClure JE, Naylor PH, et al. Isolation of thymosin alpha 1 from thymosin fraction 5 of different species by high-performance liquid chromatography. J. Chromatogr. 1983;26:533-44.
    • (1983) J. Chromatogr , vol.26 , pp. 533-544
    • Low, T.L.1    McClure, J.E.2    Naylor, P.H.3
  • 33
    • 0021207238 scopus 로고
    • Isolation and identifica-tion of thymosin alpha 1 from calf spleen using high perfor-mance liquid chromatography
    • Danho W, Gabriel TF, Makofske RC. Isolation and identifica-tion of thymosin alpha 1 from calf spleen using high perfor-mance liquid chromatography. Int. J. Pept. Protein Res. 1984;23:630-6.
    • (1984) Int. J. Pept. Protein Res , vol.23 , pp. 630-636
    • Danho, W.1    Gabriel, T.F.2    Makofske, R.C.3
  • 35
    • 0019319536 scopus 로고
    • Solid-phase synthesis of thymosin alpha 1 using tert-butyloxycarbonylaminoacyl-4-(oxymethyl) phenylacetamidomethyl-resin
    • Wong TW, Merrifield RB. Solid-phase synthesis of thymosin alpha 1 using tert-butyloxycarbonylaminoacyl-4-(oxymethyl) phenylacetamidomethyl-resin. Biochemistry. 1980;19:3233-8.
    • (1980) Biochemistry , vol.19 , pp. 3233-3238
    • Wong, T.W.1    Merrifield, R.B.2
  • 36
    • 67649094526 scopus 로고    scopus 로고
    • From lab to bedside: Emerging clinical applications of thymosin α1
    • Allan L Goldstein, Adsm L Goldstein. From lab to bedside: emerging clinical applications of thymosin α1. Expert. Opin. Biol. Ther. 2009;9:593-608.
    • (2009) Expert. Opin. Biol. Ther , vol.9 , pp. 593-608
    • Allan, L.G.1    Adsm, L.G.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.