Intact Protein Separation by One- and Two-Dimensional Liquid Chromatography for the Comparative Proteomic Separation of Partitioned Serum or Plasma

Methods in Molecular Biology

Volumn 728, Issue , 2011, Pages 29-46

  Sheng, Simon ^b   Skalnikova, Helena ^b   Meng, Andrew ^b   Tra, John ^b   Fu, Qin ^b   Everett, Allen ^b   Van Eyk, Jennifer ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b NONE

Author keywords

Biomarkers; Chromatofocusing; Liquid chromatography; Plasma; Protein load; Proteomics; Reproducibility; Reversed phase; Serum

Indexed keywords

IMMUNOGLOBULIN; IMMUNOGLOBULIN G; IMMUNOGLOBULIN Y; LIPID; PLASMA PROTEIN; SERUM ALBUMIN;

ARTICLE; CHEMISTRY; COMPARATIVE STUDY; FILTRATION; FRACTIONATION; HUMAN; ISOLATION AND PURIFICATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY; PLASMA; PROTEOMICS; REPRODUCIBILITY; REVERSED PHASE LIQUID CHROMATOGRAPHY; SERUM; ULTRACENTRIFUGATION;

BLOOD PROTEINS; CHEMICAL FRACTIONATION; CHROMATOGRAPHY, LIQUID; CHROMATOGRAPHY, REVERSE-PHASE; FILTRATION; HUMANS; IMMUNOGLOBULIN G; IMMUNOGLOBULINS; LIPIDS; MASS SPECTROMETRY; PLASMA; PROTEOMICS; REPRODUCIBILITY OF RESULTS; SERUM; SERUM ALBUMIN; ULTRACENTRIFUGATION;

EID: 79960128384     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-068-3_2     Document Type: Chapter

References (11)

1. Fu Q, Sheng S, Van Eyk JE (2007) Development of biomarker development pipeline: search for myocardial ischemia biomarkers. Book Chapter, Clinical Proteomics: From Diagnosis to Therapy, J. Van Eyk and M.J. Dunn (Eds), Wiley-VCH.
2. Schoenhoff FS, Fu Q, Van Eyk JE (2009) Cardiovascular proteomics: implications for clinical applications. Clin. Lab. Med. 29:87–99.
3. McDonald T, Sheng S, Stanley B. et al. (2006) Expanding the subproteome of the inner mitochondria using protein separation technologies: one and two-dimensional liquid chromatography and two-dimensional gel electrophoresis. Mol. Cell. Proteomics, 5:2392–2398.
4. Sheng S, Chen, D, Van Eyk JE (2006) Multidimensional liquid chromatography separation of intact protein by chromatographic focusing and reversed phase of the human serum proteome: optimization and protein database. Mol. Cell. Proteomics 5:26–34.
5. Suberbielle E, Gonzalez-Dunia D, Pont F (2008) High reproducibility of two-dimensional liquid chromatography using pH-driven fractionation with a pressure-resistant electrode. J. Chromatography B 871(1):125–9.
6. Fu Q, Garnham CP, Elliott ST, Bovenkamp DE, Van Eyk JE (2005) A robust, streamlined, and reproducible method for proteomic analysis of serum by delipidation, albumin and IgG depletion, and two-dimensional gel electrophoresis. Proteomics 5:2656–64.
7. Liu T, Qian WJ, Mottaz HM. et al. (2006) Evaluation of Multiprotein Immunoaffinity Subtraction for Plasma Proteomics and Candidate Biomarker Discovery Using Mass Spectrometry. Mol. Cell. Proteomics 5(11): 2167–74.
8. Gundry RL, Fu Q, Jelinek CA, Van Eyk JE, Cotter RJ (2007) Investigation of an albumin-enriched fraction of human serum and its albuminome. Proteomics Clin. Appl. 1:73–88.
9. Gundry RL, White MY, Murray CI, Kane LA, Fu Q, Stanley BA, Van Eyk JE (2009) Preparation of proteins and peptides for mass spectrometry analysis in a bottom-up proteomics workflow. Curr. Protoc. Mol. Biol. Chapter 10:Unit10.25
10. Huang L, Harvie G, Feitelson J. et al. (2005) Immunoaffinity separation of plasma proteins by IgY microbeads: Meeting the needs of proteomic sample preparation and analysis. Proteomics 5(13), 3314–28.
11. Gundry RL, White MY, Nogee J, Tchernyshyov I, Van Eyk JE (2009) Assessment of albumin removal from an immunoaffinity spin column: critical implications for proteomic examination of the albuminome and albumin-depleted samples. Proteomics 9:2021–8.