In Vivo labeling of zebrafish motor neurons using an mnx1 enhancer and Gal4/UAS

Genesis

Volumn 49, Issue 7, 2011, Pages 546-554

  Zelenchuk, Taras A ^a   Brusés, Juan L ^a  

^a UNIVERSITY OF KANSAS SCHOOL OF MEDICINE   (United States)

Author keywords

Danio rerio; In vivo imaging; Mnx1 enhancer; Motor neuron development; Spinal cord

Indexed keywords

DNA; ENHANCED GREEN FLUORESCENT PROTEIN; MESSENGER RNA; PLASMID DNA; PROTEIN VP16; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR GAL4; TRANSCRIPTION FACTOR MNX 1; UNCLASSIFIED DRUG; ZEBRAFISH PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL LABELING; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DENDRITE; DNA SEQUENCE; EMBRYO; ENHANCER REGION; EXPRESSION VECTOR; GENE EXPRESSION; GENOTYPE; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; LARVA; MICROINJECTION; MNX 1 GENE; MOLECULAR CLONING; NERVE FIBER GROWTH; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SEQUENCE HOMOLOGY; SPINAL CORD MOTONEURON; STABLE EXPRESSION; TRANSCRIPTION INITIATION; TRANSGENIC ANIMAL; TRANSIENT EXPRESSION; ZEBRA FISH;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; DNA-BINDING PROTEINS; ENHANCER ELEMENTS, GENETIC; GENE EXPRESSION REGULATION, DEVELOPMENTAL; MOLECULAR IMAGING; MOTOR NEURONS; NEUROGENESIS; STAINING AND LABELING; TRANSCRIPTION FACTORS; ZEBRAFISH; ZEBRAFISH PROTEINS;

ANIMALIA; DANIO RERIO;

EID: 79960127641     PISSN: 1526954X     EISSN: 1526968X     Source Type: Journal    
DOI: 10.1002/dvg.20766     Document Type: Article

References (30)

1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol 215: 403-410.
2. Arber S, Han B, Mendelsohn M, Smith M, Jessell TM, Sockanathan S. 1999. Requirement for the homeobox gene Hb9 in the consolidation of motor neuron identity. Neuron 23: 659-674.
3. Boon KL, Xiao S, McWhorter ML, Donn T, Wolf-Saxon E, Bohnsack MT, Moens CB, Beattie CE. 2009. Zebrafish survival motor neuron mutants exhibit presynaptic neuromuscular junction defects. Hum Mol Genet 18: 3615-3625.
4. Brand AH, Perrimon N. 1993. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118: 401-415.
5. Brusés J. N-cadherin regulates primary motor axons growth and branching during zebrafish embryonic development. J Comp Neurol, in press.
6. Buckley K, Kelly RB. 1985. Identification of a transmembrane glycoprotein specific for secretory vesicles of neural and endocrine cells. J Cell Biol 100: 1284-1294.
7. Collins RT, Linker C, Lewis J. 2010. MAZe: A tool for mosaic analysis of gene function in zebrafish. Nat Methods 7: 219-223.
8. Eisen JS, Myers PZ, Westerfield M. 1986. Pathway selection by growth cones of identified motoneurones in live zebra fish embryos. Nature 320: 269-271.
9. Eisen JS, Pike SH, Romancier B. 1990. An identified motoneuron with variable fates in embryonic zebrafish. J Neurosci 10: 34-43.
10. Flanagan-Steet H, Fox MA, Meyer D, Sanes JR. 2005. Neuromuscular synapses can form in vivo by incorporation of initially aneural postsynaptic specializations. Development 132: 4471-4481.
11. Fox MA, Sanes JR. 2007. Synaptotagmin I and II are present in distinct subsets of central synapses. J Comp Neurol 503: 280-296.
12. Giniger E, Varnum SM, Ptashne M. 1985. Specific DNA binding of GAL4, a positive regulatory protein of yeast. Cell 40: 767-774.
13. Hartley JL, Temple GF, Brasch MA. 2000. DNA cloning using in vitro site-specific recombination. Genome Res 10: 1788-1795.
14. Jing L, Lefebvre JL, Gordon LR, Granato M. 2009. Wnt signals organize synaptic prepattern and axon guidance through the zebrafish unplugged/MuSK receptor. Neuron 61: 721-733.
15. Kawakami K, Koga A, Hori H, Shima A. 1998. Excision of the tol2 transposable element of the medaka fish, Oryzias latipes, in zebrafish, Danio rerio. Gene 225: 17-22.
16. Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF. 1995. Stages of embryonic development of the zebrafish. Dev Dyn 203: 253-310.
17. Koster RW, Fraser SE. 2001. Tracing transgene expression in living zebrafish embryos. Dev Biol 233: 329-346.
18. Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, Yost HJ, Kanki JP, Chien CB. 2007. The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev Dyn 236: 3088-3099.
19. Matsuda T, Cepko CL. 2007. Controlled expression of transgenes introduced by in vivo electroporation. Proc Natl Acad Sci USA 104: 1027-1032.
20. Melancon E, Liu DW, Westerfield M, Eisen JS. 1997. Pathfinding by identified zebrafish motoneurons in the absence of muscle pioneers. J Neurosci 17: 7796-7804.
21. Myers PZ, Eisen JS, Westerfield M. 1986. Development and axonal outgrowth of identified motoneurons in the zebrafish. J Neurosci 6: 2278-2289.
22. Nakano T, Windrem M, Zappavigna V, Goldman SA. 2005. Identification of a conserved 125 base-pair Hb9 enhancer that specifies gene expression to spinal motor neurons. Dev Biol 283: 474-485.
23. Panzer JA, Gibbs SM, Dosch R, Wagner D, Mullins MC, Granato M, Balice-Gordon RJ. 2005. Neuromuscular synaptogenesis in wild-type and mutant zebrafish. Dev Biol 285: 340-357.
24. Sadowski I, Ma J, Triezenberg S, Ptashne M. 1988. GAL4-VP16 is an unusually potent transcriptional activator. Nature 335: 563-564.
25. Sager JJ, Bai Q, Burton EA. 2010. Transgenic zebrafish models of neurodegenerative diseases. Brain Struct Funct 214: 285-302.
26. Suster ML, Kikuta H, Urasaki A, Asakawa K, Kawakami K. 2009. Transgenesis in zebrafish with the tol2 transposon system. Methods Mol Biol 561: 41-63.
27. Tanabe Y, William C, Jessell TM. 1998. Specification of motor neuron identity by the MNR2 homeodomain protein. Cell 95: 67-80.
28. Trevarrow B, Marks DL, Kimmel CB. 1990. Organization of hindbrain segments in the zebrafish embryo. Neuron 4: 669-679.
29. Westerfield M. 2000. The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio), 4th ed. Eugene, OR: University of Oregon Press.
30. Westerfield M, McMurray JV, Eisen JS. 1986. Identified motoneurons and their innervation of axial muscles in the zebrafish. J Neurosci 6: 2267-2277.