Production and crystallization of processing α-glucosidase I: Pichia pastoris expression and a two-step purification toward structural determination

Protein Expression and Purification

Volumn 79, Issue 1, 2011, Pages 96-101

  Barker, Megan K ^a   Wilkinson, Brendan L ^b   Faridmoayer, Amirreza ^c,f   Scaman, Christine H ^c   Fairbanks, Antony J ^b,d   Rose, David R ^e  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^d UNIVERSITY OF CANTERBURY   (New Zealand)

^e UNIVERSITY OF WATERLOO   (Canada)

^f GlycoVaxyn AG   (Switzerland)

Author keywords

Alpha glucosidase; Carbohydrate synthesis; Glycoside hydrolase; N Glycosylation; Pichia pastoris; Protein crystallization; Protein expression and purification

Indexed keywords

ALPHA GLUCOSIDASE; GLUCOSIDASE I;

ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PICHIA; SACCHAROMYCES CEREVISIAE; X RAY CRYSTALLOGRAPHY;

ALPHA-GLUCOSIDASES; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; GENE EXPRESSION; MODELS, MOLECULAR; PICHIA; SACCHAROMYCES CEREVISIAE;

EUKARYOTA; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 79960121620     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.05.015     Document Type: Article

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