Development of a real-time PCR assay for detection and quantification of Rhizobium leguminosarum bacteria and discrimination between different biovars in zinc-contaminated soil

Applied and Environmental Microbiology

Volumn 77, Issue 13, 2011, Pages 4626-4633

  Macdonald, Catriona A ^a   Clark, Ian M ^a   Hirsch, Penny R ^a   Zhao, Fang Jie ^a   McGrath, Steve P ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

16S RRNA; 16S RRNA GENE; A PLANTS; DETECTION LIMITS; ENUMERATION METHOD; ENVIRONMENTAL PERTURBATIONS; FUNCTIONAL GENES; MOLECULAR APPROACH; NODULATION; PLANT BIOASSAYS; REAL-TIME PCR ASSAY; RHIZOBIUM LEGUMINOSARUM; SOIL SOLUTIONS; SOIL TYPES; TOXIC EFFECT;

BIOASSAY; GENES; POLYMERASE CHAIN REACTION; REGRESSION ANALYSIS; RNA; SEWAGE SLUDGE; SOIL MOISTURE; ZINC;

SOIL POLLUTION;

BACTERIAL PROTEIN; BACTERIAL RNA; NODD PROTEIN, BACTERIA; PRIMER DNA; RNA 16S; ZINC;

ABUNDANCE; AMMONIUM; BIOASSAY; CONCENTRATION (COMPOSITION); DISCRIMINANT ANALYSIS; GENE EXPRESSION; GENETIC ANALYSIS; LEGUME; MOLECULAR ANALYSIS; NITRATE; NODULATION; POLLUTION EFFECT; POLYMERASE CHAIN REACTION; POPULATION STRUCTURE; REGRESSION ANALYSIS; RHIZOBACTERIUM; SEWAGE; SLUDGE; SOIL MICROORGANISM; SOIL POLLUTION; SOIL TYPE; TOXICITY; ZINC;

ARTICLE; BACTERIAL LOAD; COMPARATIVE STUDY; DRUG EFFECT; EVALUATION; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; RHIZOBIUM LEGUMINOSARUM; SENSITIVITY AND SPECIFICITY; SOIL POLLUTANT;

BACTERIAL LOAD; BACTERIAL PROTEINS; DNA PRIMERS; POLYMERASE CHAIN REACTION; RHIZOBIUM LEGUMINOSARUM; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; SOIL MICROBIOLOGY; SOIL POLLUTANTS; ZINC;

RHIZOBIUM LEGUMINOSARUM;

EID: 79960095376     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02232-10     Document Type: Article

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