Specific PCR primers for the detection of isolates of Aspergillus carbonarius producing ochratoxin a on grapevine

Annals of Microbiology

Volumn 61, Issue 2, 2011, Pages 267-272

  Spadaro, Davide ^a   Patharajan, Subban ^a   Kartikeyan, Muthusamy ^a   Lorè, Alessia ^a   Garibaldi, Angelo ^a   Gullino, Maria Lodovica ^a  

^a UNIVERSITY OF TURIN   (Italy)

Author keywords

Aspergillus carbonarius; Ochratoxin A; Polyketide synthase; Vineyard

Indexed keywords

ASPERGILLUS; ASPERGILLUS CARBONARIUS; FUNGI; PENICILLIUM; VITACEAE; VITIS;

EID: 79960094808     PISSN: 15904261     EISSN: 18692044     Source Type: Journal    
DOI: 10.1007/s13213-010-0131-3     Document Type: Article

References (35)

1. Abarca ML, Bragulat MR, Castellá G, Cabañes FJ (1994) Ochratoxin A production by strains of Aspergillus niger var. niger. Appl Environ Microbiol 60:2650-2652 (Pubitemid 24205774)
2. Atoui A, Dao HP, Mathieu F, Lebrihi A (2006) Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A. Mol Nutr Food Res 50:488-493 (Pubitemid 43944156)
3. Atoui A, Mathieu F, Lebrihi A (2007) Targeting a polyketide synthase gene for Aspergillus carbonarius quantification and ochratoxin A assessment in grapes using real-time PCR. Int J Food Microbiol 115:313-318 (Pubitemid 46400883)
4. Battilani P, Pietri A, Bertuzzi T, Languasco L, Giorni P, Kozakiewicz Z (2003) Occurrence of ochratoxin A-producing fungi in grapes grown in Italy. J Food Prot 66:633-636 (Pubitemid 36429748)
5. Battilani P, Pietri A, Logrieco A (2004) Risk assessment and management in practice: ochratoxin in grapes and wine. In: Magan N, Olsen M (eds) Mycotoxins in food: detection and control. Woodhead, Cambridge, pp 244-261
6. Bellí N, Marín S, Sanchis V, Ramos AJ (2004a) Influence of water activity and temperature on growth of isolates of Aspergillus section Nigri obtained from grapes. Int J Food Microbiol 96:19-27 (Pubitemid 39195092)
7. Bellí N, Ramos AJ, Sanchis V, Marín S (2004b) Incubation time and water activity effects on ochratoxin A production by Aspergillus section Nigri strains isolated from grapes. Lett Appl Microbiol 38:72-77 (Pubitemid 38174912)
8. Bingle L E H, Simpson TJ, Lazarus CM (1999) Ketosynthase domain probes identify two subclasses of fungal polyketide synthase genes. Fungal Genet Biol 26:209-223 (Pubitemid 29412593)
9. Bragulat MR, Abarca ML, Cabañes FJ (2001) An easy screening method for fungi producing ochratoxin A in pure culture. Int J Food Microbiol 71:139-144 (Pubitemid 33123338)
10. Cox RJ, Glod F, Hurley D, Lazarus CM, Nicholson TP, Rudd B A M, Simpson TJ, Wilkinson B, Zhang Y (2004) Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis. Chem Commun 20:2260-2261 (Pubitemid 39545882)
11. Dalcero A, Magnoli C, Hallak C, Chiacchiera SM, Palacio G, Rosa C A R (2002) Detection of ochratoxin A in animal feeds and capacity to produce this mycotoxin by Aspergillus section Nigri in Argentina. Food Addit Contam 19:1065-1072 (Pubitemid 35331481)
12. Dao HP, Mathieu F, Lebrihi A (2005) Two primer pairs to detect OTA producers by PCR method. Int J Food Microbiol 104:61-67 (Pubitemid 41206451)
13. Esteban A, Abarca ML, Bragulat MR, Cabañes FJ (2004) Effects of temperature and incubation time on production of ochratoxin A by black aspergilli. Res Microbiol 155:861-866 (Pubitemid 39573357)
14. Gallo A, Perrone G, Solfrizzo M, Epifani F, Abbas A, Dobson A D W, Mulé G (2009) Characterization of a pks gene which is expressed during ochratoxin A production by Aspergillus carbonarius. Int J Food Microbiol 129:8-15
15. Geisen R (2004) Molecular monitoring of environmental conditions influencing the induction of ochratoxin A biosynthesis genes in Penicillium nordicum. Mol Nutr Food Res 48:532-540 (Pubitemid 39643696)
16. Geisen R, Mayer Z, Karolewiez A, Farber P (2004) Development of a real time PCR system for detection of Penicillium nordicum and for monitoring ochratoxin A production in foods by targeting the ochratoxin polyketide synthase gene. Syst Appl Microbiol 27:501-507 (Pubitemid 39071025)
17. Gullino ML, Lorè A, Muthusamy K, Spadaro D, Garibaldi A (2008) Monitoring of ochratoxin-producing Aspergillus carbonarius in grapevine using molecular markers. Phytopathology 98:S64
18. Horie Y (1995) Productivity of ochratoxin A of Aspergillus carbonarius in Aspergillus section Nigri. Nippon Kingakukai Kaiho 36:73-76
19. Larsen TO, Svendsen A, Smedsgaard J (2001) Biochemical characterization of ochratoxin A-producing strains of the genus Penicillium. Appl Environ Microbiol 67:3630-3635 (Pubitemid 33641235)
20. Mitchell D, Parra R, Aldred D, Magan N (2004) Water and temperature relations of growth and ochratoxin A production by Aspergillus carbonarius strains from grapes in Europe and Israel. J Appl Microbiol 97:439-445 (Pubitemid 38998677)
21. Mulè G, Susca A, Logrieco A, Stea G, Visconti A (2006) Development of a quantitative real-time PCR assay for the detection of Aspergillus carbonarius in grapes. Int J Food Microbiol 111:S28-S34 (Pubitemid 44301557)
22. Nicholson TP, Rudd B A M, Dawson M, Lazarus CM, Simpson TJ, Cox RJ (2001) Design and utility of oligonucleotide gene probes for fungal polyketide synthases. Chem Biol 8:157-178 (Pubitemid 32300210)
23. O'Callaghan J, Caddick MX, Dobson A D W (2003) A polyketide synthase required for ochratoxin A biosynthesis in Aspergillus ochraceus. Microbiology 149:3485-3491 (Pubitemid 38016676)
24. O'Callaghan J, Stapleton PC, Dobson A D W (2006) Ochratoxin A biosynthetic genes in Aspergillus ochraceus are differentially regulated by pH and nutritional stimuli. Fungal Genet Biol 43:213-221
25. Oliveri C, Torta L, Catara V (2008) A polyphasic approach to the identification of ochratoxin A-producing black Aspergillus isolates from vineyards in Sicily. Int J Food Microbiol 127:147-154
26. Patiño B, González-Salgado A, González-Jaén MT, Vázquez C (2005) PCR detection assays for the ochratoxin-producing Aspergillus carbonarius and Aspergillus ochraceus species. Int J Food Microbiol 104:207-214 (Pubitemid 41297489)
27. Perrone G, Susca A, Stea G, Mulé G (2004) PCR assay for identification of Aspergillus carbonarius and Aspergillus japonicus. Eur J Plant Pathol 110:641-649 (Pubitemid 39001465)
28. Perrone G, Susca A, Cozzi K, Ehrlich J, Varga J, Frisvad JC, Meijer M, Noomin P, Mahakarnchanakul W, Samson RA (2007) Biodiversity of Aspergillus species in some important agricultural products. Stud Mycol 59:53-66 (Pubitemid 351155587)
29. Petzinger E, Ziegler K (2000) Ochratoxin A from a toxicological perspective. J Vet Pharmacol Ther 23:91-98 (Pubitemid 30348766)
30. Pietri A, Bertuzzi T, Pallaroni L, Piva G (2001) Occurrence of ochratoxin A in Italian wines. Food Addit Contam 18:647-654 (Pubitemid 32685159)
31. Selma MV, Martínez-Culebras PV, Aznar R (2008) Real-time PCR based procedures for detection and quantification of Aspergillus carbonarius in wine grapes. Int J Food Microbiol 122:126-134
32. Spadaro D, Patharajan S, Karthikeyan M, Lorè A, Garibaldi A, Gullino ML (2009) Molecular strategies for the identification of Aspergillus species in vineyard. J Plant Pathol 91(S4):41
33. Spadaro D, Lorè A, Garibaldi A, Gullino ML (2010a) Occurrence of ochratoxin A before bottling in DOC and DOCG wines produced in Piedmont (northern Italy). Food Control 21:1294-1297
34. Spadaro D, Patharajan S, Lorè A, Gullino ML, Garibaldi A (2010b) Effect of pH, water activity and temperature on the growth and level of ochratoxin A produced by three strains of Aspergillus carbonarius isolated from Italian vineyards. Phytopathol Mediterr 49:65-73
35. Zimmerli B, Dick R (1996) Ochratoxin A in table wine and grape juice: occurrence and risk assessment. Food Addit Contam 13:655-668 (Pubitemid 26293688)