An improved protocol for optical projection tomography imaging reveals lobular heterogeneities in pancreatic islet and β-cell mass distribution

Islets

Volumn 3, Issue 4, 2011, Pages 204-208

  Hörnblad, Andreas ^a   Cheddad, Abbas ^a   Ahlgren, Ulf ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

cell mass; Contrast normalization; Islets; OPT; Pancreas

Indexed keywords

ANIMAL TISSUE; ARTICLE; C57BL 6 MOUSE; CELL STRUCTURE; CELLULAR DISTRIBUTION; HISTOGRAM; IMAGE RECONSTRUCTION; MOUSE; MOUSE STRAIN; NONHUMAN; OPTICAL PROJECTION TOMOGRAPHY; PANCREAS ISLET BETA CELL; SENSITIVITY AND SPECIFICITY; STATISTICAL ANALYSIS; TOMOGRAPHY;

ANIMALS; CELL SIZE; COMPUTATIONAL BIOLOGY; FEMALE; IMAGE ENHANCEMENT; IMAGING, THREE-DIMENSIONAL; INSULIN-SECRETING CELLS; ISLETS OF LANGERHANS; MICE; MICE, INBRED C57BL; ORGAN SIZE; PANCREAS; PANCREAS, EXOCRINE; REPRODUCIBILITY OF RESULTS; TOMOGRAPHY, OPTICAL;

EID: 79960091945     PISSN: 19382014     EISSN: 19382022     Source Type: Journal    
DOI: 10.4161/isl.3.4.16417     Document Type: Article

References (16)

1. Hagai H. Configurational anatomy of the pancreas: its surgical relevance from ontogenetic and comparative-anatomical viewpoints. J Hepatobiliary Pancreat Surg 2003; 10:48-56.
2. Hellerstrom C, Hellman B. The islets of Langerhans in yellow obese mice. Metabolism 1963; 12:527-36.
3. Hellman B, Brolin S, Hellerstrom C, Hellman K. The distribution pattern of the pancreatic islet volume in normal and hyperglycaemic mice. Acta Endocrinol (Copenh) 1961; 36:609-16.
4. Sharpe J, Ahlgren U, Perry P, Hill B, Ross A, Hecksher-Sorensen J, et al. Optical projection tomography as a tool for 3D microscopy and gene expression studies. Science 2002; 296:541-5. (Pubitemid 34408675)
5. Sharpe J. Optical projection tomography. Annu Rev Biomed Eng 2004; 6:209-28. (Pubitemid 39209497)
6. Alanentalo T, Asayesh A, Morrison H, Loren CE, Holmberg D, Sharpe J, et al. Tomographic molecular imaging and 3D quantification within adult mouse organs. Nat Methods 2007; 4:31-3. (Pubitemid 46029471)
7. Alanentalo T, Hornblad A, Mayans S, Karin Nilsson A, Sharpe J, Larefalk A, et al. Quantification and three-dimensional imaging of the insulitis-induced destruction of beta-cells in murine type 1 diabetes. Diabetes 2010; 59:1756-64.
8. Sun G, Tarasov AI, McGinty JA, French PM, McDonald A, Leclerc I, et al. LKB1 deletion with the RIP2.Cre transgene modifies pancreatic beta-cell morphology and enhances insulin secretion in vivo. Am J Physiol Endocrinol Metab 298:1261-73.
9. Zuiderveld K. Contrast limited adaptive histogram equalization. Graphic Gems IV. Boston: Academic Press Professional 1994; 474-85.
10. Pisano ED, Zong S, Hemminger BM, DeLuca M, Johnston RE, Muller K, et al. Contrast limited adaptive histogram equalization image processing to improve the detection of simulated spiculations in dense mammograms. J Digit Imaging 1998; 11:193-200. (Pubitemid 128541943)
11. Pizer SM, Amburn EP, Austin JD, Cromartie R, Geselowitz A, Greer T, et al. Adaptive histogram equalization and its variations. Computer Vision Graphics and Image Processing 1987; 39:355-68. (Pubitemid 17655926)
12. Pizer SM, Zimmerman JB, Staab EV. Adaptive grey level assignment in CT scan display. J Comput Assist Tomogr 1984; 8:300-5. (Pubitemid 14169678)
13. Bock T, Pakkenberg B, Buschard K. Genetic background determines the size and structure of the endocrine pancreas. Diabetes 2005; 54:133-7. (Pubitemid 40105104)
14. Holmberg D, Ahlgren U. Imaging the pancreas: from ex vivo to non-invasive technology. Diabetologia 2008; 51:2148-54.
15. Huang HH, Novikova L, Williams SJ, Smirnova IV, Stehno-Bittel L. Low insulin content of large islet population is present in situ and in isolated islets. Islets 2011; 3:6-13.
16. Alanentalo T, Loren CE, Larefalk A, Sharpe J, Holmberg D, Ahlgren U. High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas. J Biomed Opt 2008; 13:054070.