Target-Enrichment Through Amplification of Hairpin-Ligated Universal Targets for Next-Generation Sequencing Analysis

Methods in Molecular Biology

Volumn 733, Issue , 2011, Pages 267-278

  Singh, Pallavi ^b   Nayak, Rajesh ^b   Kwon, Young Min ^a  

^a UNIVERSITY OF ARKANSAS   (United States)

^b NONE

Author keywords

Hairpin selector; Multiple targets; Next generation sequencing; PCR amplification; Target enrichment

Indexed keywords

DNA; PRIMER DNA; RESTRICTION ENDONUCLEASE;

ARTICLE; BACTERIAL GENOME; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; DNA SEQUENCE; ENZYME SPECIFICITY; GENETICS; INVERTED REPEAT; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SALMONELLA;

BASE SEQUENCE; DNA; DNA PRIMERS; DNA RESTRICTION ENZYMES; GENOME, BACTERIAL; INVERTED REPEAT SEQUENCES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SALMONELLA; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

EID: 79960048278     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-089-8_19     Document Type: Chapter

References (7)

1. Metzker, M. L. (2010) Sequencing technologies – the next generation. Nat. Rev. Genet. 11, 31–46.
2. Mamanova, L., Coffey, A. J., Scott, C. E., Kozarewa, I., Turner, E. H., Kumar, A., Howard, E., Shendure, J., and Turner, D. J. (2010) Target-enrichment strategies for next-generation sequencing. Nat. Methods 7, 111–118.
3. Dahl, F., Stenberg, J., Fredriksson, S., Welch, K., Zhang, M., Nilsson, M., Bicknell, D., Bodmer, W. F., Davis, R. W., and Ji, H. (2007) Multigene amplification and massively parallel sequencing for cancer mutation discovery. Proc. Natl. Acad. Sci. USA. 104, 9387–9392.
4. Varley, K. E., and Mitra, R. D. (2008) Nested Patch PCR enables highly multiplexed mutation discovery in candidate genes. Genome Res. 18, 1844–1850.
5. Kotetishvili, M., Stine, O. C., Kreger, A., Morris, J. G. Jr, and Sulakvelidze, A.(2002) Multilocus sequence typing for characterization of clinical and environmental salmonella strains. J Clin Microbiol. 40, 1626–1635.
6. Sukhnanand, S., Alcaine, S., Warnick, L. D., Su, W. L., Hof, J., Craver, M. P., McDonough, P., Boor, K. J., and Wiedmann, M. (2005) DNA sequence-based subtyping and evolutionary analysis of selected Salmonella enterica serotypes. J Clin Microbiol. 43, 3688–3698.
7. Tankouo-Sandjong, B., Sessitsch, A., Liebana, E., Kornschober, C., Allerberger, F., Hächler, H., and Bodrossy, L. (2007) MLST-v, multilocus sequence typing based on virulence genes, for molecular typing of Salmonella enterica subsp. enterica serovars. J Microbiol Methods. 69, 23–36.