Immersion freezing of suspended particles and cells for cryo-electron microscopy

Cold Spring Harbor Protocols

Volumn 6, Issue 7, 2011, Pages 803-814

  Resch, Guenter P ^a   Brandstetter, Marlene ^a   Königsmaier, Lisa ^a   Urban, Edit ^a   Pickl Herk, Angela M ^b  

^a INSTITUTE OF MOLECULAR BIOTECHNOLOGY   (Austria)

^b MEDICAL UNIVERSITY OF VIENNA   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

LIPOSOME;

ARTICLE; CELL ISOLATION; CELL ORGANELLE; CELL SCREENING; CELL SUSPENSION; CRYO TRANSMISSION ELECTRON MICROSCOPY; CYTOSKELETON; FREEZING; IMMERSION; PROTEIN ANALYSIS; REPRODUCIBILITY; SUSPENDED PARTICULATE MATTER; TRANSMISSION ELECTRON MICROSCOPY; VIRUS; CELL; CRYOELECTRON MICROSCOPY; CRYOPRESERVATION; METHODOLOGY; PARTICULATE MATTER; SUSPENSION; ULTRASTRUCTURE;

CELLS; CRYOELECTRON MICROSCOPY; CRYOPRESERVATION; IMMERSION; PARTICULATE MATTER; SUSPENSIONS;

EID: 79960040459     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5642     Document Type: Article

References (14)

1. Aebi U, Pollard TD. 1987. A glow discharge unit to render electron microscope grids and other surfaces hydrophilic. J Electron Microsc Tech 7: 29-33.
2. Crucifix C, Papai G, Schultz P. 2009. Frozen-Hydrated Macromolecules for Structural Analysis. In Handbook of cryo-preparation methods for electron microscopy (ed. A Cavalier et al.), pp. 161-192. CRC Press, Taylor & Francis Group, Boca Raton, FL.
3. Dobro MJ, Melanson LA, Jensen GJ, McDowall AW. 2010. Plunge freezing for electron cryomicroscopy. Methods Enzymol 481: 63-82.
4. Fischer N, Konevega AL, Wintermeyer W, Rodnina MV, Stark H. 2010. Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy. Nature 466: 329-333.
5. Frederik PM, Hubert DH. 2005. Cryoelectron microscopy of liposomes. Methods Enzymol 391: 431-448.
6. Frederik PM, de Haas F, Storms MMH. 2009. Controlled Vitrification. In Handbook of cryo-preparation methods for electron microscopy (ed. A Cavalier et al.), 71-102. CRC Press, Taylor & Francis Group, Boca Raton, FL.
7. Grassucci RA, Taylor DJ, Frank J. 2007. Preparation of macromolecular complexes for cryo-electron microscopy. Nat Protoc 2: 3239-3246.
8. Hasanovic A, Winkler R, Resch GP, Valenta C. 2011. Modification of the conformational skin structure by treatment with liposomal formulations and its correlation to the penetration depth of aciclovir. Eur J Pharm Biopharm doi: 10.1016/ejbp.2011.01.018.
9. Iancu CV, Tivol WF, Schooler JB, Dias DP, Henderson GP, Murphy GE, Wright ER, Li Z, Yu Z, Briegel A, et al. 2007. Electron cryotomography sample preparation using the Vitrobot. Nat Protoc 1: 2813-2819.
10. Mastronarde DN. 2005. Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152: 36-51.
11. Resch GP, Brandstetter M, Pickl-Herk AM, Königsmaier L, Wonesch VI, Urban E. 2011a. Immersion freezing of biological specimens: rationale, principles, and instrumentation. Cold Spring Harb Protoc doi: 10.1101/pdb.top118.
12. Resch GP, Brandstetter M, Wonesch VI, Urban E. 2011b. Immersion freezing of cell monolayers for cryo-electron tomography. Cold Spring Harb Protoc doi: 10.1101/pdb.prot5643.
13. Suloway C, Pulokas J, Fellmann D, Cheng A, Guerra F, Quispe J, Stagg S, Potter CS, Carragher B. 2005. Automated molecular microscopy: the new Leginon system. J Struct Biol 151: 41-60.
14. Wagner S, Königsmaier L, Lara-Tejero M, Lefebre M, Marlovits TC, Galán JE. 2010. Organization and coordinated assembly of the type III secretion export apparatus. Proc Natl Acad Sci 107: 17745-17750.