Intact rat superior mesenteric artery endothelium is an electrical syncytium and expresses strong inward rectifier K+ conductance

Biochemical and Biophysical Research Communications

Volumn 410, Issue 3, 2011, Pages 501-507

  Climent, Belén ^a   Zsiros, Emese ^a,b   Stankevicius, Edgaras ^c   Villa, Pedro de la ^d   Panyi, György ^b   Simonsen, Ulf ^c   García Sacristán, Albino ^a   Rivera, Luis ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b UNIVERSITY OF DEBRECEN   (Hungary)

^c AARHUS UNIVERSITY   (Denmark)

^d UNIVERSITY OF ALCALÁ   (Spain)

Author keywords

Gap junctions; Intact endothelial cell; KIR channels; Patch clamp in situ

Indexed keywords

18BETA GLYCYRRETINIC ACID; ACETYLCHOLINE; BARIUM CHLORIDE; DECAY ACCELERATING FACTOR; DYE; INWARDLY RECTIFYING POTASSIUM CHANNEL; N ACETYLPENICILLAMINE; RETINOIC ACID; S NITROSO N ACETYL PENICILLAMINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTERY ENDOTHELIUM; ARTICLE; BLOOD VESSEL CAPACITANCE; CELL MEMBRANE CONDUCTANCE; CHANNEL GATING; CONTROLLED STUDY; ELECTROPHYSIOLOGY; ENDOTHELIUM CELL; GAP JUNCTION; ION CURRENT; ION TRANSPORT; MALE; MEMBRANE CONDUCTANCE; MEMBRANE STEADY POTENTIAL; NONHUMAN; PATCH CLAMP; POTASSIUM CONDUCTANCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; RESISTANCE BLOOD VESSEL; SUPERIOR MESENTERIC ARTERY; VASODILATATION; WHOLE CELL; WHOLE CELL BLIND PATCH RECORDING;

ACETYLCHOLINE; ANIMALS; CELLS, CULTURED; ELECTRIC CONDUCTIVITY; ENDOTHELIUM, VASCULAR; GAP JUNCTIONS; GIANT CELLS; MEMBRANE POTENTIALS; MESENTERIC ARTERIES; POTASSIUM CHANNELS, INWARDLY RECTIFYING; RATS; S-NITROSO-N-ACETYLPENICILLAMINE;

RATTUS;

EID: 79960034685     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2011.06.011     Document Type: Article

References (27)

1. Köhler R., Brakemeier S., Kuhn M., Degenhardt C., Buhr H., Pries A., Hoyer J. Expression of ryanodine receptor type 3 and TRP channels in endothelial cells: comparison of in situ and cultured human endothelial cells. Cardiovasc. Res. 2001, 51:160-168.
2. Tang G., Wang R. Differential expression of KV and KCa channels in vascular smooth muscle cells during 1-day culture. Pflügers Arch. 2001, 442:124-135.
3. Luo G., Xu C.B., Cao Y.X., Edvinsson L. Transcriptional up-regulation in expression of 5-hydroxytryptamine2A and transcriptional down-regulation of angiotensin II type 1 receptors during organ culture of rat mesenteric artery. Basic Clin. Pharmacol. Toxicol. 2004, 95:280-287.
4. Sandow S.L., Grayson T.H. Limits of isolation and culture: intact vascular endothelium and BKCa. Am. J. Physiol. Heart Circ. Physiol. 2009, 97:H1-H7.
5. Yamamoto Y., Fukuta H., Nakahira Y., Suzuki H. Blockade by 18-ß-glycyrrhetinic acid of intercellular electrical coupling in guinea-pig arterioles. J. Physiol. 1998, 511:501-508.
6. Yamamoto Y., Imaeda K., Suzuki H. Endothelium-dependent hyperpolarization and intercellular electrical coupling in guinea-pig mesenteric arterioles. J. Physiol. 1999, 514:505-513.
7. Yamamoto Y., Klemm M.F., Edwards F.R., Suzuki H. Intercellular electrical communication among smooth muscle and endothelial cells in guinea-pig mesenteric arterioles. J. Physiol. 2001, 535:181-195.
8. Zhang Q., Cao C., Mangano M., Zhang Z., Silldorff E.P., Lee-Kwon W., Payne K., Pallone T.L. Descending vasa recta endothelium is an electrical syncytium. Am. J. Physiol. Regul. Integr. Comp. Physiol. 2006, 291:R1688-R1699.
9. de Wit C. Different pathways with distinct properties conduct dilations in the microcirculation in vivo. Cardiovasc. Res. 2010, 85:604-613.
10. Figueroa X.F., Duling B.R. Gap junctions in the control of vascular function. Antioxid. Redox Signal. 2009, 11:251-266.
11. Crane G.J., Neild T.O., Segal S.S. Contribution of active membrane processes to conducted hyperpolarization in arterioles of hamster cheek pouch. Microcirculation 2005, 11:425-433.
12. Jantzi M.C., Brett S.E., Jackson W.F., Corteling R., J Vigmond E., Welsh D.G. Inward rectifying potassium channels facilitate cell-to-cell communication in hamster retractor muscle feed arteries. Am. J. Physiol. Heart Circ. Physiol. 2006, 291:H1319-H1328.
13. Smith P.D., Brett S.E., Luykenaar K.D., Sandow S.L., Marrelli S.P., Vigmond E., Welsh D. KIR channels function as electrical amplifiers in rat vascular smooth muscle. J. Physiol. 2008, 586:1147-1160.
14. Marchenko S.M., Sage S.O. Smooth muscle cells affect endothelial membrane potential in rat aorta. Am. J. Physiol. 1994, 267:H804-H811.
15. Selemidis S., Cocks T. Smooth muscle mediates circumferential conduction of hyperpolarization and relaxation to focal endothelial cell activation in large coronary arteries. Naunyn Schmiedebergs Arch. Pharmacol. 2007, 375:85-94.
16. Germaín F., Fernandez E., de la Villa P. Morphometrical analysis of dendritic arborization in axotomized retinal ganglion cells. Eur. J. NeuroSci. 2003, 18:1103-1109.
17. Stankevicius E., Lopez-Valverde V., Rivera L., Hughes A.D., Mulvany M.J., Simonsen U. Combination of Ca2+-activated K+ channel blockers inhibits acetylcholine-evoked nitric oxide release in rat superior mesenteric artery. Br. J. Pharmacol. 2006, 149:560-572.
18. Mulvany M.J., Halpern W. Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circ. Res. 1997, 41:19-26.
19. Simonsen U., García-Sacristán A., Prieto D. Apamin-sensitive K+ channels involved in the inhibition of acetylcholine-induced contractions in lamb coronary small arteries. Eur. J. Pharmacol. 1997, 329:153-163.
20. Beny J.L., Gribi F. Dye and electrical coupling of endothelial cells in situ. Tissue Cell 1989, 21:797-802.
21. Matchkov V.V., Rahman A., Peng H., Nilsson H., Aalkjaer C. Junctional and nonjunctional effects of heptanol and glycyrrhetinic acid derivates in rat mesenteric small arteries. Br. J. Pharmacol. 2004, 142:961-972.
22. Tare M., Coleman H.A., Parkington H.C. Glycyrrhetinic derivatives inhibit hyperpolarization in endothelial cells of guinea pig and rat arteries. Am. J. Physiol. Heart Circ. Physiol. 2002, 282:H335-H341.
23. Nilius B., Droogmans G. Ion channels and their functional role in vascular endothelium. Physiol. Rev. 2001, 81:1415-1459.
24. Nelson M.T., Quayle J.M. Physiological roles and properties of potassium channels in arterial smooth muscle. Am. J. Physiol. Cell. Physiol. 1995, 268:C799-C822.
25. Crane G.J., Walker S.D., Dora K.A., Garland C.J. Evidence for a differential cellular distribution of inward rectifier K channels in the rat isolated mesenteric artery. J. Vasc. Res. 2003, 40:159-168.
26. Rivers R.J., Hein T.W., Zhang C., Kuo L. Activation of barium-sensitive inward rectifier potassium channels mediates remote dilation of coronary arterioles. Circulation 2001, 104:1749-1753.
27. Takano H., Dora K.A., Spitaler M.M., Garland C.J. Spreading dilatation in rat mesenteric arteries associated with calcium-independent endothelial cell hyperpolarization. J. Physiol. 2004, 556:887-903.