Empirical model and in vivo characterization of the bacterial response to synthetic gene expression show that ribosome allocation limits growth rate

Biotechnology Journal

Volumn 6, Issue 7, 2011, Pages 773-783

  Carrera, Javier ^a,b,c   Rodrigo, Guillermo ^a,b   Singh, Vijai ^a   Kirov, Boris ^a   Jaramillo, Alfonso ^a  

^a UNIVERSITÉ D EVRY VAL D ESSONNE   (France)

^b Ciudad Politécnica de la Innovación   (Spain)

^c UNIVERSITAT POLITÈCNICA DE VALÈNCIA   (Spain)

Author keywords

Cell resources; Cellular chassis modeling; Heterologous system; Synthetic biology

Indexed keywords

BACTERIAL CELLS; CELLULAR CHASSIS MODELING; CELLULAR PARAMETERS; CELLULAR RESOURCES; COPY NUMBER; CULTURE CONDITIONS; EMPIRICAL MODEL; FLUORESCENT MEASUREMENTS; FLUORESCENT REPORTER; HETEROLOGOUS EXPRESSION; HETEROLOGOUS SYSTEM; HETEROLOGOUS SYSTEMS; IN-VIVO; RNA POLYMERASE; SYNTHETIC BIOLOGY; TOTAL PROTEIN;

BACTERIOLOGY; CELL CULTURE; CHASSIS; FLUORESCENCE; GROWTH KINETICS; MACROMOLECULES; MATHEMATICAL MODELS; RNA;

GENE EXPRESSION;

DNA POLYMERASE; ISOPROPYL THIOGALACTOSIDE; RNA POLYMERASE; YELLOW FLUORESCENT PROTEIN;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; CELL FUNCTION; CELL GROWTH; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GROWTH RATE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN RESTRICTION; RIBOSOME; SYNTHETIC BIOLOGY;

CELL ENLARGEMENT; CULTURE MEDIA; ESCHERICHIA COLI; GENE EXPRESSION; GENES, REPORTER; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; PLASMIDS; REPRODUCIBILITY OF RESULTS; RIBOSOMES; SYNTHETIC BIOLOGY; SYSTEMS BIOLOGY;

BACTERIA (MICROORGANISMS);

EID: 79959936732     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201100084     Document Type: Article

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