Sensitive and specific phenotypic assay for metallo-β-lactamase detection in enterobacteria by use of a moxalactam disk supplemented with EDTA

Journal of Clinical Microbiology

Volumn 49, Issue 7, 2011, Pages 2667-2670

  Pluquet, Emilie ^a,b   Arlet, Guillaume ^c,d   Bingen, Edouard ^e,f   Drieux, Laurence ^g   Mammeri, Hedi ^a,b  

^a AMIENS UNIVERSITY HOSPITAL   (France)

^b UNIVERSITÉ DE PICARDIE JULES VERNE   (France)

^c HÔPITAL TENON   (France)

^d SORBONNE UNIVERSITÉ   (France)

^e HÔPITAL ROBERT DEBRÉ   (France)

^f UNIVERSITÉ PARIS DESCARTES   (France)

^g ASSISTANCE PUBLIQUE HÔPITAUX DE PARIS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BETA LACTAMASE; BETA LACTAMASE ACC 1; BETA LACTAMASE CMY 4; BETA LACTAMASE CTX M 14; BETA LACTAMASE CTX M 15; BETA LACTAMASE CTX M 9; BETA LACTAMASE KPC 2; BETA LACTAMASE NDM 1; BETA LACTAMASE OXA 1; BETA LACTAMASE OXA 48; BETA LACTAMASE OXA 9; BETA LACTAMASE SHV 12; BETA LACTAMASE SHV 5; BETA LACTAMASE TEM 1; BETA LACTAMASE VIM 1; BETA LACTAMASE VIM 4; CEFEPIME; CEFOTAXIME; CEFTAZIDIME; DORIPENEM; EDETIC ACID; ERTAPENEM; IMIPENEM; LATAMOXEF; MEROPENEM; METALLO BETA LACTAMASE; UNCLASSIFIED DRUG;

ACCURACY; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CONTROLLED STUDY; CULTURE MEDIUM; DISK POTENTIATION TEST; ENTEROBACTER CLOACAE; ENTEROBACTERIACEAE; ENZYME ASSAY; ENZYME SYNTHESIS; ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEUS MIRABILIS; PROVIDENCIA STUARTII; SENSITIVITY AND SPECIFICITY; TEST STRIP;

ENTEROBACTERIACEAE;

EID: 79959831967     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00328-11     Document Type: Article

References (25)

1. Bidet, P., et al. 2005. In vivo transfer of plasmid-encoded ACC-1 AmpC from Klebsiella pneumoniae to Escherichia coli in an infant and selection of impermeability to imipenem in K. pneumoniae. Antimicrob. Agents Chemother. 49:3562-3565.
2. Cao, V. T. B. C., et al. 2000. Emergence of imipenem resistance in Klebsiella pneumoniae owing to combination of plasmid-mediated CMY-4 and permeability alteration. J. Antimicrob. Chemother. 46:895-900.
3. Chu, Y. W., T. K. Cheung, J. Y. Ngan, and K. M. Kam. 2005. EDTA susceptibility leading to false detection of metallo-β-lactamase in Pseudomonas aeruginosa by Etest and an imipenem-EDTA disk method. Int. J. Antimicrob. Agents 26:340-341.
4. Clinical and Laboratory Standards Institute. 2010. Performance standards for antimicrobial susceptibility testing: 20th informational supplement. Approved standard M100-S20. Clinical and Laboratory Standards Institute, Wayne, PA.
5. Clinical and Laboratory Standards Institute. June 2010 update. Performance standards for antimicrobial susceptibility testing: 20th informational supplement. Approved standard M100-S20-U. CLSI, Wayne, PA.
6. Cohen Stuart, J., M. A. Leverstein-Van Hall, on behalf of members of the Dutch Working Party on the Detection of Highly Resistant Microorganisms. 2010. Guideline for phenotypic screening and confirmation of carbapenemases in Enterobacteriaceae. Int. J. Antimicrob. Agents 36:205-210.
7. Decré, D., et al. 2010. Possible importation and subsequent cross-transmission of OXA-48-producing Klebsiella pneumoniae, France, 2010. Euro Surveill. 15:19718-19719.
8. Dortet, L., et al. 2008. Intercontinental travels of patients and dissemination of plasmid-mediated carbapenemase KPC-3 associated with OXA-9 and TEM-1. J. Antimicrob. Chemother. 61:455-457.
9. Drieux, L., et al. 2011. Accumulation of carbapenemase-producing Gramnegative in a single patient linked to the acquisition of multiple carbapenemase producers and to the in vivo transfer of a plasmid encoding VIM-1. Int. J. Antimicrob. Agents. [Epub ahead of print.] doi:10.1016/j.ijantimicag. 2011.03.017.
10. SHV-5 in a French university hospital. J. Antimicrob. Chemother. 57:142-145.
11. Kassis-Chikhani, N., et al. 2010. Outbreak of Klebsiella pneumoniae producing KPC-2 and SHV-12 in a French hospital. J. Antimicrob. Chemother. 65:1539-1540.
12. Ktari, S., et al. 2006. Emergence of multidrug-resistant Klebsiella pneumoniae isolates producing VIM-4 metallo-β-lactamase, CTX-M-15 extended-spectrum β-lactamase, and CMY-4 AmpC β-lactamase in a Tunisian university hospital. Antimicrob. Agents Chemother. 50:4198-4201.
13. Kumarasamy, K. K., et al. 2010. Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect. Dis. 10:597-602.
14. Mammeri, H., H. Guillon, F. Eb, and P. Nordmann. 2010. Phenotypic and biochemical comparison of the carbapenem-hydrolyzing activities of five plasmid-borne AmpC β-lactamases. Antimicrob. Agents Chemother. 64:4556-4560.
15. Nordmann, P., L. Poirel, A. Carrër, M. A. Toleman, and T. R. Walsh. 2011. How to detect NDM-1 producers? J. Clin. Microbiol. 49:718-721.
16. Philippon, A., G. Arlet, and G. Jacoby. 2002. Plasmid-determined AmpCtype β-lactamases. Antimicrob. Agents Chemother. 46:1-11.
17. Poirel, L., L. Briñas, A. Verlinde, L. Ide, and P. Nordmann. 2005. BEL-1, a novel clavulanic acid-inhibited extended-spectrum β-lactamase, and the class 1 integron In120 in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 49:3743-3748.
18. Poirel, L., C. Héritier, C. Spicq, and P. Nordmann. 2004. In vivo acquisition of high-level resistance to imipenem in Escherichia coli. J. Clin. Microbiol. 42:3831-3833.
19. Poirel, L., E. Lagrutta, P. Taylor, J. Pham, and P. Nordmann. 2010. Emergence of metallo-β-lactamase NDM-1-producing multidrug-resistant Escherichia coli in Australia. Antimicrob. Agent Chemother. 54:4914-4916.
20. Poirel, L., Z. Al Maskari, F. Al Rashdi, S. Bernabeu, and P. Nordmann. 2011. NDM-1-producing Klebsiella pneumoniae isolated in the Sultana of Oman. J. Antimicrob. Chemother. 66:304-306.
21. Spencer, J., et al. 2005. Antibiotic recognition by binuclear metallo-β-lactamases revealed by X-ray crystallography. J. Am. Chem. Soc. 127:14439-14444.
22. Tan, J., J. D. D. Pitout, and D. S. Guttman. 2008. New and sensitive assay for determining Pseudomonas aeruginosa metallo-β-lactamase resistance to imipenem. J. Clin. Microbiol. 46:1870-1872.
23. Thomson, K. S. 2010. Extended-spectrum β-lactamases, AmpC, and carbapenemase issues. J. Clin. Microbiol. 48:1019-1025.
24. Vercheval, L., et al. 2010. Three factors that modulate the activity of class D β-lactamases and interfere with the post-translational carboxylation of Lys70. Biochem. J. 432:495-504.
25. Walsh, T. R., M. A. Toleman, L. Poirel, and P. Nordmann. 2005. Metallo-β-lactamases: the quiet before the storm? Clin. Microbiol. Rev. 18:306-325.