A high-throughput immobilized bead screen for stable proteins and multi-protein complexes

Protein Engineering, Design and Selection

Volumn 24, Issue 7, 2011, Pages 565-578

  Lockard, Meghan A ^a   Listwan, Pawel ^a   Pedelacq, Jean Denis ^a,b,c   Cabantous, Stéphanie ^a,d,e,f   Nguyen, Hau B ^a   Terwilliger, Thomas C ^a   Waldo, Geoffrey S ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

^b INSTITUT DE PHARMACOLOGIE ET DE BIOLOGIE STRUCTURALE   (France)

^c UNIVERSITÉ PAUL SABATIER   (France)

^d CANCER RESEARCH CENTER OF TOULOUSE   (France)

^e UNIVERSITÉ DE TOULOUSE   (France)

^f INSTITUT CLAUDIUS REGAUD   (France)

Author keywords

chemical lysis; high throughput screening; IMAC beads; protein tagging; split GFP

Indexed keywords

AGAROSE; BREAKPOINT; COEXPRESSED; COMPLEMENTATION; DNA FRAGMENT; FILTRATION MEMBRANES; GREEN FLUORESCENT PROTEIN; HIGH-THROUGHPUT; HIGH-THROUGHPUT SCREENING; HUMAN PROTEINS; IMAC BEADS; IN-VITRO; METAL AFFINITY; MULTI-PROTEIN COMPLEX; N-TERMINALS; PROTEIN TAGGING; SOLUBLE PROTEINS; SPLIT GFP;

ESCHERICHIA COLI; FLUORESCENCE; MICROFILTRATION;

PROTEINS;

AGAROSE; DNA FRAGMENT; GREEN FLUORESCENT PROTEIN; HU PROTEIN; MULTIPROTEIN COMPLEX; POLYHISTIDINE TAG; PROTEIN P85; RESIN; SYNAPSIN I;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM COLONY; CARBOXY TERMINAL SEQUENCE; CLONE; ESCHERICHIA COLI; FILTRATION; FLUORESCENCE; HUMAN; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION;

CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENE LIBRARY; GREEN FLUORESCENT PROTEINS; HIGH-THROUGHPUT SCREENING ASSAYS; HISTIDINE; HUMANS; MULTIPROTEIN COMPLEXES; MYCOBACTERIUM SMEGMATIS; OLIGOPEPTIDES; PROTEIN STABILITY; PYROBACULUM; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 79959725135     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzr021     Document Type: Article

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