A novel flow cytometry-based assay for the quantification of Staphylococcus aureus adhesion to and invasion of eukaryotic cells

Journal of Microbiological Methods

Volumn 86, Issue 2, 2011, Pages 145-149

  Trouillet, Sophie ^a,b   Rasigade, Jean Philippe ^a,b   Lhoste, Yannick ^a,b   Ferry, Tristan ^a,b   Vandenesch, François ^a   Etienne, Jerome ^a   Laurent, Frederic ^a,b  

^a UNIVERSITÉ DE LYON   (France)

^b HOSPICES CIVILS DE LYON   (France)

Author keywords

Bacterial adhesion; Bacterial fibronectin binding proteins; Bacterial invasion; Flow cytometry; Host pathogen interactions; Staphylococcus aureus

Indexed keywords

FIBRONECTIN BINDING PROTEIN; FIBRONECTIN BINDING PROTEIN ALPHA; FIBRONECTIN BINDING PROTEIN BETA; GENTAMICIN; LYSOSTAPHIN; UNCLASSIFIED DRUG;

ARTICLE; CELL ADHESION; CELL INVASION; CONTROLLED STUDY; EUKARYOTE; FLOW CYTOMETRY; GENE DELETION; HOST PATHOGEN INTERACTION; INTERMETHOD COMPARISON; INTERNALIZATION; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; STAPHYLOCOCCUS AUREUS;

ADHESINS, BACTERIAL; BACTERIAL ADHESION; CELL LINE; FLOW CYTOMETRY; GENE DELETION; HUMANS; OSTEOBLASTS; STAPHYLOCOCCUS AUREUS; VIRULENCE FACTORS;

BACTERIA (MICROORGANISMS); EUKARYOTA; STAPHYLOCOCCUS AUREUS;

EID: 79959713600     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.04.012     Document Type: Article

References (17)

1. Agerer F., Waeckerle S., Hauck C.R. Microscopic quantification of bacterial invasion by a novel antibody-independent staining method. J. Microbiol. Methods 2004, 59:23-32.
2. Ahmed S., M.S., Williams R.J., Henderson B., Brock J.H., Nair S.P. Staphylococcus aureus fibronectin binding proteins are essential for internalization by osteoblasts but do not account for differences in intracellular levels of bacteria. Infect. Immun. 2001, 60:2872-2877.
3. Berendt T., Byren I. Bone and joint infection. Clin. Med. 2004, 4:510-518.
4. Dassy B., Stringfellow W.T., Lieb M., Fournier J.M. Production of type 5 capsular polysaccharide by Staphylococcus aureus grown in a semi-synthetic medium. J. Gen. Microbiol. 1991, 137:1155-1162.
5. Dehart H.P., Heath H.E., Heath L.S., Leblanc P.A., Sloan G.L. The lysostaphin endopeptidase resistance gene (epr) specifies modification of peptidoglycan cross bridges in Staphylococcus simulans and Staphylococcus aureus. Appl. Environ. Microbiol. 1995, 61:2811.
6. DeLoid G.M., Sulahian T.H., Imrich A., Kobzik L. Heterogeneity in macrophage phagocytosis of Staphylococcus aureus strains: high-throughput scanning cytometry-based analysis. PLoS One 2009, 4:e6209.
7. Ellington J.K., Reilly S.S., Ramp W.K., Smeltzer M.S., Kellam J.F., Hudson M.C. Mechanisms of Staphylococcus aureus invasion of cultured osteoblasts. Microb. Pathog. 1999, 26:317-323.
8. Foster T.J., Hook M. Surface protein adhesins of Staphylococcus aureus. Trends Microbiol. 1998, 6:484-488.
9. Greene C., McDevitt D., Francois P., Vaudaux P., Lew D., Foster T. Adhesion properties of mutants of Staphylococcus aureus defective in fibronectin-binding proteins and studies on the expression of fnb genes. Mol. Microbiol. 1995, 17:1143-1152.
10. Jevon M., Guo C., Ma B., Mordan N., Nair S.P., Harris M., Henderson B., Bentley G., Meghji S. Mechanisms of internalization of Staphylococcus aureus by cultured human osteoblasts. Infect. Immun. 1999, 67:2677-2681.
11. Kusuma C., Jadanova A., Chanturiya T., Kokai-Kun J.F. Lysostaphin-resistant variants of Staphylococcus aureus demonstrate reduced fitness in vitro and in vivo. Antimicrob. Agents. Chemother. 2007, 51:475-482.
12. Pils S., Schmitter T., Neske F., Hauck C.R. Quantification of bacterial invasion into adherent cells by flow cytometry. J. Microbiol. Methods 2006, 65:301-310.
13. Sinha B., Francois P., Nube O., Foti M., Hartford O.M., Vaudaux P., Foster T.J., Lew D.P., Herrmann M., Krause K.H. Fibronectin-binding protein acts as Staphylococcus aureus invasion via fibronectin bridging to integrin α5β1. Cell. Microbiol. 1999, 1:101-107.
14. Sinha B., Francois P., Que Y.A., Hussain M., Heilmann C., Moreillon P., Lew D., Krause K.H., Peters G., Herrmann M. Heterologously expressed Staphylococcus aureus fibronectin-binding proteins are sufficient for invasion of host cells. Infect. Immun. 2000, 68:6871-6878.
15. Stevens Q.E., Seibly J.M., Chen Y.H., Dickerman R.D., Noel J., Kattner K.A. Reactivation of dormant lumbar methicillin-resistant Staphylococcus aureus osteomyelitis after 12years. J. Clin. Neurosci. 2007, 14:585-589.
16. Stringfellow W.T., Dassy B., Lieb M., Fournier J.M. Staphylococcus aureus growth and type 5 capsular polysaccharide production in synthetic media. Appl. Environ. Microbiol. 1991, 57:618-621.
17. Wright K.M., Friedland J.S. Regulation of chemokine gene expression and secretion in Staphylococcus aureus-infected osteoblasts. Microbes. Infect. 2004, 6:844-852.