A fluorescence-based polymerase chain reaction-linked single-strand conformation polymorphism (F-PCR-SSCP) assay for the identification of Fasciola spp.

Parasitology Research

Volumn 108, Issue 6, 2011, Pages 1513-1517

  Alasaad, Samer ^a   Soriguer, Ramón C ^a   Abu Madi, Marawan ^b   El Behairy, Ahmed ^c   Baños, Pablo Díez ^d   Píriz, Ana ^a   Fickel, Joerns ^e   Zhu, Xing Quan ^f  

^a DEPARTMENT OF CONSERVATION BIOLOGY   (Spain)

^b Qatar University   (Qatar)

^c Cairo University   (Egypt)

^d Universidade de Santiago de Compostela   (Spain)

^e LEIBNIZ INSTITUTE FOR ZOO AND WILDLIFE RESEARCH   (Germany)

^f Lanzhou Veterinary Research Institute   (China)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; FLUORESCENT DYE; INTERNAL TRANSCRIBED SPACER 2; RIBOSOME DNA;

ACCURACY; ARTICLE; CAPILLARY ELECTROPHORESIS; CHEMICAL LABELING; CHINA; CONTROLLED STUDY; DNA SEQUENCE; EGYPT; FASCIOLA; FASCIOLA GIGANTICA; FASCIOLA HEPATICA; FLUORESCENCE ANALYSIS; GENE AMPLIFICATION; GENETIC MARKER; INTERMEDIATE HOST; MOLECULAR SIZE; NIGERIA; NONHUMAN; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS DEVELOPMENT; SINGLE STRAND CONFORMATION POLYMORPHISM; SPAIN; SPECIES DIFFERENCE;

ANIMALS; DNA PRIMERS; DNA, HELMINTH; DNA, RIBOSOMAL SPACER; ELECTROPHORESIS, CAPILLARY; FASCIOLA; FLUORESCENT DYES; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL;

ANIMALIA; FASCIOLA; FASCIOLA GIGANTICA; FASCIOLA HEPATICA;

EID: 79959374960     PISSN: 09320113     EISSN: 14321955     Source Type: Journal    
DOI: 10.1007/s00436-010-2209-z     Document Type: Article

References (41)

1. T Agatsuma Y Arakawa M Iwagami Y Honzako U Cahyaningsih SY Kang SJ Hong 2000 Molecular evidence of natural hybridization between Fasciola hepatica and F. gigantica Parasitol Int 49 231 238 (Pubitemid 30665647)
2. L Ai SJ Dong WY Zhang HM Elsheikha YS Mahmmod RQ Lin ZG Yuan YL Shi WY Huang XQ Zhu 2010 Specific PCR-based assays for the identification of Fasciola species: their development, evaluation and potential usefulness in prevalence surveys Ann Trop Med Parasitol 104 65 72
3. S Alasaad CQ Huang QY Li JE Granados C García-Romero JM Pérez XQ Zhu 2007 Characterisation of Fasciola samples from different host species and geographical localities in Spain by sequences of internal transcribed spacers of rDNA Parasitol Res 101 1245 1250 (Pubitemid 47389426)
4. S Alasaad JE Granados FJ Cano-Manuel A Meana XQ Zhu JM Pérez 2008 Epidemiology of fasciolosis affecting Iberian ibex, Capra pyrenaica, from Sierra Nevada massif, southern Spain Parasitol Res 102 751 755
5. K Ashrafi MA Valero M Panova MV Periago J Massoud S Mas-Coma 2006 Phenotypic analysis of adults of Fasciola hepatica, Fasciola gigantica and intermediate forms from the endemic region of Gilan, Iran Parasitol Int 55 249 260 (Pubitemid 44636311)
6. M Bernat E Titos J Clària 2002 Rapid identification of single nucleotide polymorphisms by fluorescence-based capillary electrophoresis Genet Mol Res 1 72 78 (Pubitemid 38977592)
7. MA Cucher S Carnevale L Prepelitchi JH Labbé C Wisnivesky-Colli 2006 PCR diagnosis of Fasciola hepatica in field-collected Lymnaea columella and Lymnaea viatrix snails Vet Parasitol 137 74 82 (Pubitemid 43384029)
8. RB Gasser 1997 Mutation scanning methods for the analysis of parasite genes Int J Parasitol 27 1449 1463 (Pubitemid 28026754)
9. RB Gasser 2006 Molecular tools-advances, opportunities and prospects Vet Parasitol 36 69 89
10. RB Gasser NB Chilton 2001 Applications of single-strand conformation polymorphism (SSCP) to taxonomy, diagnosis, population genetics and molecular evolution of parasitic nematodes Vet Parasitol 101 201 213 (Pubitemid 33052450)
11. RB Gasser M Hu NB Chilton BE Campbell AJ Jex D Otranto C Cafarchia I Beveridge XQ Zhu 2006 Single-strand conformation polymorphism (SSCP) for the genetic analysis and identification of pathogens Nat Protoc 1 3121 3128
12. KR Gunasekar AK Tewari C Sreekumar SC Gupta JR Rao 2008 Elucidation of genetic variability among different isolates of Fasciola gigantica (giant liver fluke) using random-amplified polymorphic DNA polymerase chain reaction Parasitol Res 103 1075 1081
13. TA Hall 1999 BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT Nucleic Acids Symp Ser 41 95 98
14. O Handt M Höss M Krings S Pääbo 1994 Ancient DNA-methodological challenges Experientia 50 524 529
15. AN Haseeb AM el-Shazly MA Arafa AT Morsy 2002 A review on fascioliasis in Egypt J Egypt Soc Parasitol 32 317 354
16. WY Huang B He CR Wang XQ Zhu 2004 Characterisation of Fasciola species from mainland China by ITS-2 ribosomal DNA sequence Vet Parasitol 120 75 83 (Pubitemid 38270385)
17. Y Ishii F Nakamura-Uchiyama Y Nawa 2002 A praziquantel ineffective fascioliasis case successfully treated with triclabendazole Parasitol Int 51 205 209 (Pubitemid 34756824)
18. T Itagaki K Tsutsumi 1998 Triploid form of Fasciola in Japan: genetic relationships between Fasciola hepatica and Fasciola gigantica determined by ITS-2 sequence of nuclear rDNA Int J Parasitol 28 777 781 (Pubitemid 28266908)
19. W Jianping M Lehto A Berglund LC Groop 2001 An automated fluorescent single strand conformation polymorphism technique for high throughput mutation screening Chin Med J 114 1147 1150 (Pubitemid 33100143)
20. N Jones H Ougham H Thomas I Pasakinskiene 2009 Markers and mapping revisited: finding your gene New Phytol 183 935 966
21. RA Kane J Bartley JR Stothard J Vercruysse D Rollinson VR Southgate 2002 Application of single strand conformational polymorphism (SSCP) analysis with fluorescent primers for differentiation of Schistosoma haematobium group species Trans R Soc Trop Med Hyg 96 Suppl S235 S241
22. SB Kendall 1965 Relationships between the species of Fasciola and their molluscan hosts Adv Parasitol 3 59 98
23. F Krämer T Schnieder 1998 Sequence heterogeneity in a repetitive DNA element of Fasciola Int J Parasitol 28 1923 1929 (Pubitemid 29017727)
24. TH Le NV De T Agatsuma TG Thi Nguyen QD Nguyen DP McManus D Blair 2008 Human fascioliasis and the presence of hybrid/introgressed forms of Fasciola hepatica and Fasciola gigantica in Vietnam Int J Parasitol 38 725 730 (Pubitemid 351452025)
25. AX Li S D'Amello L Paggl F He RB Gasser ZR Lun E Abollo M Turchetto XQ Zhu 2005 Genetic evidence for the existence of sibling species within Contracaecum rudolphii Hartwich, 1964 and the validity of Contracaecum septentrionale Kreis, 1955 (Nematoda: Anisakidae) Parasitol Res 96 361 366 (Pubitemid 41095411)
26. RQ Lin SJ Dong K Nie CR Wang AX Li HQ Song WY Huang XQ Zhu 2007 Sequence analysis of the first internal transcribed spacer of rDNA supports the existence of an intermediate Fasciola between F. hepatica and F. gigantica in mainland China Parasitol Res 101 813 817 (Pubitemid 47101413)
27. KG Magalhães LK Jannotti-Passos RL Caldeira ME Berne G Muller OS Carvalho HL Lenzi 2008 Isolation and detection of Fasciola hepatica DNA in Lymnaea viatrix from formalin-fixed and paraffin-embedded tissues through multiplex-PCR Vet Parasitol 152 333 338
28. A Marcilla MD Bargues S Mas-Coma 2002 A PCR-RFLP assay for the distinction between Fasciola hepatica and Fasciola gigantica Mol Cell Probes 16 327 333 (Pubitemid 35397435)
29. S Mas-Coma MD Bargues 1997 Human liver flukes: a review Res Rev Parasitol 57 145 218
30. S Mas-Coma JG Esteban MD Bargues 1999 Epidemiology of human fascioliasis: a review and proposed new classification Bull Wld Hlth Org 77 340 346 (Pubitemid 29282843)
31. S Mas-Coma MD Bargues MA Valero 2005 Fascioliasis and other plant-borne trematode zoonoses Int J Parasitol 35 1255 1278 (Pubitemid 41395182)
32. S Mas-Coma MA Valero MD Bargues 2009 Chapter 2. Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control Adv Parasitol 69 41 146
33. AC Pasqualotto DW Denning MJ Anderson 2007 A cautionary tale: lack of consistency in allele sizes between two laboratories for a published multilocus microsatellite typing system J Clin Microbiol 45 522 528
34. MV Periago MA Valero M El Sayed K Ashrafi A El Wakeel MY Mohamed M Desquesnes F Curtale S Mas-Coma 2008 First phenotypic description of Fasciola hepatica/Fasciola gigantica intermediate forms from the human endemic area of the Nile Delta, Egypt Infect Genet Evol 8 51 58 (Pubitemid 350225339)
35. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana, Totowa, pp 365-386
36. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual
37. TW Spithill JP Dalton 1998 Progress in development of liver fluke vaccines Parasitol Today 14 224 822
38. A Susca G Stea G Perrone 2007 Rapid polymerase chain reaction (PCR)-single-stranded conformational polymorphism (SSCP) screening method for the identification of Aspergillus section Nigri species by the detection of calmodulin nucleotide variations Food Addit Contam 24 1148 1153 (Pubitemid 47484748)
39. R Velusamy BP Singh OK Raina 2004 Detection of Fasciola gigantica infection in snails by polymerase chain reaction Vet Parasitol 120 85 90 (Pubitemid 38270386)
40. Yamaguti S (1958) Systema helminthum, vol. I. The digenetic trematodes of vertebrates. Interscience, New York
41. XQ Zhu S D'Amelio HW Palm L Paggi M George-Nascimento RB Gasser 2002 SSCP-based identification of members within the Pseudoterranova decipiens complex (Nematoda: Ascaridoidea: Anisakidae) using genetic markers in the internal transcribed spacers of ribosomal DNA Parasitology 124 615 623 (Pubitemid 34747955)