Novel post-translational modifications of the hemagglutinin and neuraminidase proteins of avian influenza virus expressed by Kluyveromyces lactis

Journal of Virological Methods

Volumn 175, Issue 2, 2011, Pages 175-181

  Tsai, Su Ming ^a   Chiang, Yu Chung ^b   Chin, Li Tzu ^c   Liu, Hung Jen ^a   Wang, Chi Young ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^b NATIONAL SUN YAT SEN UNIVERSITY   (Taiwan)

^c NATIONAL PINGTUNG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Taiwan)

Author keywords

Avian influenza virus; HA and NA proteins; Kluyveromyces lactis

Indexed keywords

INFLUENZA VIRUS HEMAGGLUTININ; VIRUS SIALIDASE;

ARTICLE; AVIAN INFLUENZA VIRUS; CONTROLLED STUDY; DEGLYCOSYLATION; ENZYME ACTIVITY; FUNGAL GENOME; GENE INSERTION; IMMUNOASSAY; KLUYVEROMYCES LACTIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN HYDROLYSIS; PROTEIN PROCESSING; VIRUS GENOME; VIRUS ISOLATION;

GENE EXPRESSION; GLYCOSYLATION; HEMAGGLUTININ GLYCOPROTEINS, INFLUENZA VIRUS; INFLUENZA A VIRUS, H5N2 SUBTYPE; KLUYVEROMYCES; NEURAMINIDASE; PROTEIN BINDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; VIRAL PROTEINS;

AVIAN INFLUENZA VIRUS; KLUYVEROMYCES LACTIS; ORTHOMYXOVIRIDAE;

EID: 79959358915     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.05.006     Document Type: Article

References (23)

1. Ballou C.E. Isolation, characterization and properties of Saccharomyces cerevisae mnn mutants with nonconditional protein glycosylation defects. Methods Enzymol. 1990, 185:440-476.
2. Bottcher E., Matrosovich T., Beyerle M., Klenk H., Garten W., Matrosovich M. Proteolytic activation of influenza viruses by serine proteases TMPRSS2 and HAT from human airway epithelium. J. Virol. 2006, 80:9896-9898.
3. Chan K.H., Maldeis N., Pope W., Yup A., Ozinsksa A., Guill J., Seto W.H., Shortridge K.F., Peiris J.S. Evaluation of the Directigen FluA+B test for rapid diagnosis of influenza virus type A and B infections. J. Clin. Microbiol. 2002, 40:1675-1680.
4. Choi Y.K., Nguyen T.D., Ozaki H., Webby R.J., Puthavathana P., Buranathal C., Chaisingh A., Auewarakul P., Hanh N.T.H., Ma S.K., Hui P.Y., Guan Y., Peiris J.S.M., Webster R.G. Studies on H5N1 influenza virus infection of pigs by using viruses isolated in Vietnam and Thailand in 2004. J. Virol. 2005, 79:10821-10825.
5. De Vries R.P., De Vries E., Bosch B.J., De Groot R.J., Rottier P.J.M., De Haan C.A. The influenza A virus hemagglutinin glycosylation state affects receptor-binding specificity. Virology 2010, 403:17-25.
6. Horimoto T., Takada A., Iwatsuki-Horimoto K., Hatta M., Goto H., Kawaoka Y. Generation of influenza A viruses with chimeric (Type A/B) hemagglutinins. J. Virol. 2003, 77:8031-8038.
7. Hu Y.C., Luo Y.L., Ji W.T., Chulu J.L.C., Chang P.C., Shieh H., Wang C.Y., Liu H.J. Dual expression of the HA protein of H5N2 avian influenza virus in a baculovirus system. J. Virol. Methods 2006, 135:43-48.
8. Kawaoka Y., Webster R.G. Interplay between carbohydrate in the stalk and the length of the connecting peptide determines the cleavability of influenza virus hemagglutinin. J. Virol. 1989, 63:3296-3300.
9. Matrosovich M.N., Matrosovichi T.Y., Gray T., Roberts N.A., Klenk H.D. Neuraminidase is important for the initiation of influenza virus infection in human airway epithelium. J. Virol. 2004, 78:12665-12667.
10. Mishin V.P., Novikov D., Hayden F.G., Gubareva L.V. Effect of hemagglutinin glycosylation on influenza virus susceptibility to neuraminidase inhibitors. J. Virol. 2005, 79:12416-12424.
11. Read J.D., Colussi P.A., Ganatra M.B., Taron C.H. Acetamide selection of Kluyveromyces lactis cells transformed with an integrative vector leads to high-frequency formation of multicopy strains. Appl. Environ. Microbiol. 2007, 73:5088-5096.
12. Rocha S.N., Abrahao-Neto J., Cerdan M.E., Gonzalez-Siso M.I., Gombert A.K. Heterologous expression of glucose oxidase in the yeast Kluyveromyces marixianus. Microb. Cell Fact. 2010, 9:4.
13. Su B., Wurtzer S., Rameix-Welti M., Dwyer D., van der Werf S., Naffakh N., Clavel F., Labrosse B. Enhancement of the influenza A hemagglutinin HA-mediated cell-cell fusion and virus entry by the viral neuraminidase (NA). PLoS One 2009, 4:e8495.
14. Takahashi K., Yuuki T., Takai T., Ra C., Okumura K., Yokota T., Okumura Y. Production of humanized Fab fragment against human high affinity IgE receptor in Pichia pastoris. Biosci. Biotechnol. Biochem. 2000, 64:2138-2144.
15. Trimble R.B., Lubowski C., Hauer C.R., Stack R., McNaughton L., Gemmill T.R., Kumar S.A. Characterization of N- and O-linked glycosylation of recombinant human bile salt-stimulated lipase secreted by Pichia pastoris. Glycobiology 2004, 14:265-274.
16. Ucelletti D., Farina F., Rufini S., Magnelli P., Abeijon C., Palleschi C. The kluyveromyces lactis α1,6-mannosyltransferase KlOch1p is required for cell-wall organization and proper functioning of the secretary pathway. FEMS Yeast Res. 2006, 6:449-457.
17. Upadhyay C., Ammayappan A., Vakharia V.N. Detection of NP, N3 and N7 antibodies to avain influenza virus by indirect ELISA using yeast-expressed antigens. Virol. J. 2009, 6:158.
18. Wang C.Y., Luo Y.L., Chen Y.T., Li S.K., Lin C.H., Hsieh Y.C., Liu H.J. The cleavage of the hemagglutinin protein of H5N2 avain influenza virus in yeast. J. Virol. Methods 2007, 146:293-297.
19. Wood G.W., McCauley J.W., Bashiruddin J.B., Alexander D.J. Deduced amino acid sequences at the hemagglutinin cleavage site of avian influenza A viruses of H5 and H7 subtypes. Arch. Virol. 1993, 130:209-217.
20. Wu H., Williams K., Singh S.R., Scissum-Gunn K., Singh N.K., Teresa D., Giambrone J.J. Integrity of a recombinant hemagglutinin protein of an avian influenza virus. Biotechnol. Lett. 2009, 31:1511-1517.
21. Yang P., Bansal A., Liu C., Air G.M. Hemagglutinin specificity and neuraminidase coding capacity of neuraminidase-deficient influenza viruses. Virology 1997, 229:155-165.
22. Yang D.G., Chung Y.C., Lai Y.K., Lai C.W., Liu H.J., Hu Y.C. Display of avian influenza virus hemagglutinin on baculovirus envelope: effect of cytoplasmic domain on virus properties and potentials as vaccine. Mol. Ther. 2007, 15(5):989-996.
23. Yang M., Berhane Y., Salo T., Li M., Hole K., Clavijo A. Development and application of monoclonal antibodies against avian influenza virus nucleoprotein. J. Virol. Methods 2008, 147:265-274.