Cadmium-inducible BgMT2, a type 2 metallothionein gene from mangrove species (Bruguiera gymnorrhiza), its encoding protein shows metal-binding ability

Journal of Experimental Marine Biology and Ecology

Volumn 405, Issue 1-2, 2011, Pages 128-132

  Huang, Guo Yong ^a,b   Wang, You Shao ^a   Ying, Guang Guo ^b  

^a SOUTH CHINA SEA INSTITUTE OF OCEANOLOGY   (China)

^b GUANGZHOU INSTITUTE OF GEOCHEMISTRY   (China)

Author keywords

GST fusion proteins; Heavy metal stress; Heavy metal tolerance; Mangrove; Metallothioneins

Indexed keywords

CADMIUM; COLIFORM BACTERIUM; COPPER; DETOXIFICATION; DICOTYLEDON; GENE; LEAD; MANGROVE; METABOLISM; POLYMERASE CHAIN REACTION; PROTEIN; REAL TIME; TOLERANCE; ZINC;

BACTERIA (MICROORGANISMS); BRUGUIERA GYMNORHIZA; ESCHERICHIA COLI;

EID: 79959347893     PISSN: 00220981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jembe.2011.05.034     Document Type: Article

References (28)

1. Bilecen K., Ozturk U.H., Duru A.D., Sutlu T., Petoukhov M.V., Svergun D.I., Koch M.H., Sezerman U.O., Cakmak I., Sayers Z. Triticum durum metallothionein: Isolation of the gene and structural characterization of the protein using solution scattering and molecular modeling. J. Biol. Chem. 2005, 280:13701-13711.
2. Castiglione S., Franchin C., Fossati T., Lingua G., Torrigiani P., Biondi S. High zinc concentrations reduce rooting capacity and alter metallothionein gene expression in white poplar (Populus alba L. cv. Villafranca). Chemosphere 2007, 67:1117-1126.
3. Chatthai M., Osusky M., Osuska L., Yevtushenko D., Misra S. Functional analysis of a Douglas-fir metallothionein-like gene promoter: transient assays in zygotic and somatic embryos and stable transformation in transgenic tobacco. Planta 2004, 220:118-128.
4. Cobbett C., Goldsbrough P.B. Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annu. Rev. Plant Biol. 2002, 53:159-182.
5. Foley R.C., Liang Z.M., Singh K.B. Analysis of type 1 metallothionein cDNAs in Vicia faba. Plant Mol. Biol. 1997, 33:583-591.
6. Gonzalez-Mendoza D., Moreno A.Q., Zapata-Perez O. Coordinated responses of phytochelatin synthase and metallothionein genes in black mangrove, Avicennia germinans, exposed to cadmium and copper. Aquat. Toxicol. 2007, 83:306-314.
7. Guo W.J., Bundithya W., Goldsbrough P.B. Characterization of the Arabidopsis metallothionein gene family: tissue-specific expression and induction during senescence and in response to copper. New Phytol. 2003, 159:369-381.
8. Huang G.Y., Wang Y.S. Expression analysis of type 2 metallothionein gene in mangrove species (Bruguiera gymnorrhiza) under heavy metal stress. Chemosphere 2009, 77:1026-1029.
9. Huang G.Y., Wang Y.S. Expression and characterization analysis of type 2 metallothionein from grey mangrove species (Avicennia marina) in response to heavy metal stress. Aquat. Toxicol. 2010, 99:86-92.
10. Huang G.Y., Wang Y.S. Physiological and biochemical responses in the leaves of two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza) exposed to multiple heavy metals. J. Hazard. Mater. 2010, 182:848-854.
11. Huang G.Y., Wang Y.S., Sun C.C., Dong J.D., Sun Z.X. The effect of multiple heavy metals on ascorbate, glutathione and related enzymes in two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza). Oceanol. Hydrobiol. Stud. 2010, 39:11-25.
12. Kohler A., Blaudez D., Chalot M., Martin F. Cloning and expression of multiple metallothioneins from hybrid poplar. New Phytol. 2004, 164:83-93.
13. Lane B., Kajoika R., Kennedy R. The wheat-germ Ec protein is a zinc-containing metallothionein. Biochem. Cell Biol. 1987, 65:1001-1005.
14. Lee J., Shim D., Song W.Y., Hwang I., Lee Y. Arabidopsis metallothioneins 2a and 3 enhance resistance to cadmium when expressed in Vicia faba guard cells. Plant Mol. Biol. 2004, 54:805-815.
15. μμμCT method. Methods 2001, 25:402-408.
16. Mir G., Domenech J., Huguet G., Guo W.J., Goldsbrough P., Atrian S., Molinas M. A plant type 2 MT from cork tissue responds to oxidative stress. J. Exp. Bot. 2004, 55:2483-2493.
17. Murphy A., Zhou J. Purification and immunological identification of metallothioneins 1 and 2 from Arabidopsis thaniana. Plant Physiol. 1997, 113:1293-1301.
18. Ranieri A., Castagna A., Scebba F., Careri M., Zagnoni I., Predieri G., Pagliari M., di Toppi L.S. Oxidative stress and phytochelatin characterisation in bread wheat exposed to cadmium excess. Plant Physiol. Biochem. 2005, 43:45-54.
19. Ren Y.J., Zhao J. Functional analysis of the rice metallothionein gene OsMT2b promoter in transgenic Arabidopsis plants and rice germinated embryos. Plant Sci. 2009, 176:528-538.
20. Robinson N.J., Tommey A.M., Kuske C., Jackson P.J. Plant metallothioneins. Biochem. J. 1993, 295:1-10.
21. Schor-Fumbarov T., Goldsbrough P.B., Adam Z., Tel-Or E. Characterization and expression of a metallothionein gene in the aquatic fern Azolla filiculoides under heavy metal stress. Planta 2005, 223:69-76.
22. Usha B., Prashanth S.R., Parida A. Differential expression of two metallothionein encoding genes during heavy metal stress in the mangrove species, Avicennia marina (Forsk.) Vierh. Curr. Sci. 2007, 93:1215-1219.
23. Usha B., Venkataraman G., Parida A. Heavy metal and abiotic stress inducible metallothionein isoforms from Prosopis juliflora (SW) D.C. show differences in binding to heavy metals in vitro. Mol. Genet. Genomics 2009, 281:99-108.
24. Yang Z., Wu Y., Li Y., Ling H.Q., Chu C. OsMT1a, a type 1 metallothionein, plays the pivotal role in zinc homeostasis and drought tolerance in rice. Plant Mol. Biol. 2009, 70:219-229.
25. Zhang Y.W., Tam N.F.Y., Wong Y.S. Cloning and characterization of type 2 metallothionein-like gene from a wetland plant Typha latifolia. Plant Sci. 2004, 167:869-877.
26. Zhang F.Q., Wang Y.S., Lou Z.P., Dong J.D. Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza). Chemosphere 2007, 67:44-50.
27. Zhigang A., Cuijie L., Yuangang Z., Yejie D., Wachter A., Gromes R., Rausch T. Expression of BjMT2, a metallothionein 2 from Brassica juncea, increases copper and cadmium tolerance in Escherichia coli and Arabidopsis thaliana, but inhibits root elongation in Arabidopsis thaliana seedlings. J. Exp. Bot. 2006, 57:3575-3582.
28. Zhou J., Goldsbrough P.B. Functional homologs of fungal metallothionein genes from Arabidopsis. Plant Cell 1994, 6:875-884.