Deep sequencing on genome-wide scale reveals the unique composition and expression patterns of microRNAs in developing pollen of Oryza sativa

Genome Biology

Volumn 12, Issue 6, 2011, Pages

  Wei, Li Q ^a,b   Yan, Long F ^a,b,c   Wang, Tai ^a,b  

^a INSTITUTE OF BOTANY   (China)

^b INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

[No Author keywords available]

Indexed keywords

MICRORNA; PLANT RNA;

ARTICLE; DEVELOPMENTAL STAGE; GENE EXPRESSION PROFILING; GENE SEQUENCE; GENETIC CONSERVATION; GENOME ANALYSIS; MICROSPORE; MULTIGENE FAMILY; NONHUMAN; PLANT GENOME; POLLEN DEVELOPMENT; PREDICTION; PYROSEQUENCING; RICE; RNA ANALYSIS; RNA SEQUENCE; SPOROPHYTE; BIOLOGY; CHEMISTRY; CLUSTER ANALYSIS; GENE EXPRESSION REGULATION; GENETICS; HIGH THROUGHPUT SEQUENCING; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLLEN; REPRODUCIBILITY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS;

MAGNOLIOPHYTA; ORYZA SATIVA;

BASE SEQUENCE; CLUSTER ANALYSIS; COMPUTATIONAL BIOLOGY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, PLANT; GENOME, PLANT; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; MICRORNAS; MOLECULAR SEQUENCE DATA; ORYZA SATIVA; POLLEN; REPRODUCIBILITY OF RESULTS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, RNA;

EID: 79959262495     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/gb-2011-12-6-r53     Document Type: Article

References (87)

1. Voinnet O. Origin, biogenesis, and activity of plant microRNAs. Cell 2009, 136:669-687. 10.1016/j.cell.2009.01.046, 19239888.
2. Schwach F, Moxon S, Moulton V, Dalmay T. Deciphering the diversity of small RNAs in plants: the long and short of it. Brief Funct Genomic Proteomic 2009, 8:472-481. 10.1093/bfgp/elp024, 19641088.
3. Jones-Rhoades MW, Bartel DP, Bartel B. MicroRNAS and their regulatory roles in plants. Annu Rev Plant Biol 2006, 57:19-53. 10.1146/annurev.arplant.57.032905.105218, 16669754.
4. Borg M, Brownfield L, Twell D. Male gametophyte development: a molecular perspective. J Exp Bot 2009, 60:1465-1478. 10.1093/jxb/ern355, 19213812.
5. Wei LQ, Xu WY, Deng ZY, Su Z, Xue Y, Wang T. Genome-scale analysis and comparison of gene expression profiles in developing and germinated pollen in Oryza sativa. BMC Genomics 2010, 11:338. 10.1186/1471-2164-11-338, 2895629, 20507633.
6. Pina C, Pinto F, Feijo JA, Becker D. Gene family analysis of the Arabidopsis pollen transcriptome reveals biological implications for cell growth, division control, and gene expression regulation. Plant Physiol 2005, 138:744-756. 10.1104/pp.104.057935, 1150393, 15908605.
7. Slotkin RK, Vaughn M, Borges F, Tanurdzic M, Becker JD, Feijo JA, Martienssen RA. Epigenetic reprogramming and small RNA silencing of transposable elements in pollen. Cell 2009, 136:461-472. 10.1016/j.cell.2008.12.038, 2661848, 19203581.
8. Borges F, Gomes G, Gardner R, Moreno N, McCormick S, Feijo JA, Becker JD. Comparative transcriptomics of Arabidopsis sperm cells. Plant Physiol 2008, 148:1168-1181. 10.1104/pp.108.125229, 2556834, 18667720.
9. Grant-Downton R, Hafidh S, Twell D, Dickinson HG. Small RNA pathways are present and functional in the angiosperm male gametophyte. Mol Plant 2009, 2:500-512. 10.1093/mp/ssp003, 19825633.
10. Grant-Downton R, Trionnaire GL, Schmid R, Rodriguez-Enriquez J, Said Hafidh2 SM, Twell D, Dickinson H. MicroRNA and tasiRNA diversity in mature pollen of Arabidopsis thaliana. BMC Genomics 2009, 10:643. 10.1186/1471-2164-10-643, 2808329, 20042113.
11. Chambers C, Shuai B. Profiling microRNA expression in Arabidopsis pollen using microRNA array and real-time PCR. BMC Plant Biol 2009, 9:87. 2715406, 19591667.
12. Henderson IR, Zhang XY, Lu C, Johnson L, Meyers BC, Green PJ, Jacobsen SE. Dissecting Arabidopsis thaliana DICER function in small RNA processing, gene silencing and DNA methylation patterning. Nat Genet 2006, 38:721-725. 10.1038/ng1804, 16699516.
13. Kato M, de Lencastre A, Pincus Z, Slack FJ. Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during Caenorhabditis elegans development. Genome Biol 2009, 10:R54. 10.1186/gb-2009-10-5-r54, 2718520, 19460142.
14. Reddy AM, Zheng Y, Jagadeeswaran G, Macmil SL, Graham WB, Roe BA, Desilva U, Zhang WX, Sunkar R. Cloning, characterization and expression analysis of porcine microRNAs. BMC Genomics 2008, 10:65. 2644714, 19196471.
15. Li R, Li Y, Kristiansen K, Wang J. SOAP: Short Oligonucleotide Alignment Program. Bioinformatics (Oxford) 2008, 24:713-714.
16. Griffiths-Jones S, Grocock RJ, van Dongen S, Bateman A, Enright AJ. miRBase: microRNA sequences, targets and gene nomenclature. Nucleic Acids Res 2006, 34:D140-144. 10.1093/nar/gkj112, 1347474, 16381832.
17. Griffiths-Jones S. The microRNA registry. Nucleic Acids Res 2004, 32:D109-111. 10.1093/nar/gkh023, 308757, 14681370.
18. Griffiths-Jones S, Saini HK, van Dongen S, Enright AJ. miRBase: tools for microRNA genomics. Nucleic Acids Res 2008, 36:D154-158. 10.1093/nar/gkn221, 2238936, 17991681.
19. Girard A, Hannon GJ. Conserved themes in small-RNA-mediated transposon control. Trends Cell Biol 2008, 18:136-148. 10.1016/j.tcb.2008.01.004, 2995447, 18282709.
20. Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T. The small RNA profile during Drosophila melanogaster development. Dev Cell 2003, 5:337-350. 10.1016/S1534-5807(03)00228-4, 12919683.
21. Kurihara Y, Watanabe Y. Arabidopsis micro-RNA biogenesis through Dicer-like 1 protein functions. Proc Natl Acad Sci USA 2004, 101:12753-12758. 10.1073/pnas.0403115101, 515125, 15314213.
22. Reinhart BJ, Weinstein EG, Rhoades MW, Bartel B, Bartel DP. MicroRNAs in plants. Genes Dev 2002, 16:1616-1626. 10.1101/gad.1004402, 186362, 12101121.
23. Meyers BC, Axtell MJ, Bartel B, Bartel DP, Baulcombe D, Bowman JL, Cao X, Carrington JC, Chen XM, Green PJ, Griffiths-Jones S, Jacobsen SE, Mallory AC, Martienssen RA, Poethig RS, Qi Y, Vaucheret H, Voinnet O, Watanabe Y, Weigel D, Zhu JK. Criteria for annotation of plant microRNAs. Plant Cell 2008, 20:3186-3190. 10.1105/tpc.108.064311, 2630443, 19074682.
24. Fahlgren N, Howell MD, Kasschau KD, Chapman EJ, Sullivan CM, Cumbie JS, Givan SA, Law TF, Grant SR, Dangl JL, Carrington JC. High-throughput sequencing of Arabidopsis microRNAs: evidence for frequent birth and death of MIRNA genes. PLoS One 2007, 2:e219. 10.1371/journal.pone.0000219, 1790633, 17299599.
25. Sunkar R, Zhou X, Zheng Y, Zhang W, Zhu JK. Identification of novel and candidate miRNAs in rice by high throughput sequencing. BMC Plant Biol 2008, 8:25. 10.1186/1471-2229-8-25, 2292181, 18312648.
26. Rajagopalan R, Vaucheret H, Trejo J, Bartel DP. A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana. Genes Dev 2006, 20:3407-3425. 10.1101/gad.1476406, 1698448, 17182867.
27. Zhang G, Guo G, Hu X, Zhang Y, Li Q, Li R, Zhuang R, Lu Z, He Z, Fang X, Chen L, Tian W, Tao Y, Kristiansen K, Zhang X, Li S, Yang H, Wang J, Wang J. Deep RNA sequencing at single base-pair resolution reveals high complexity of the rice transcriptome. Genome Res 2010, 20:646-654. 10.1101/gr.100677.109, 2860166, 20305017.
28. Ouyang S, Buell CR. The TIGR Plant Repeat Databases: a collective resource for the identification of repetitive sequences in plants. Nucleic Acids Res 2004, 32:D360-D363. 10.1093/nar/gkh099, 308833, 14681434.
29. Zhang YJ. miRU: an automated plant miRNA target prediction server. Nucleic Acids Res 2005, 33:W701-W704. 10.1093/nar/gki383, 1160144, 15980567.
30. Mallory AC, Vaucheret H. Functions of microRNAs and related small RNAs in plants. Nat Genet 2006, 38:S31-S36. 10.1038/ng1791, 16736022.
31. Lu C, Jeong DH, Kulkarni K, Pillay M, Nobuta K, German R, Thatcher SR, Maher C, Zhang L, Ware D, Liu B, Cao X, Meyers BC, Green PJ. Genome-wide analysis for discovery of rice microRNAs reveals natural antisense microRNAs (nat-miRNAs). Proc Natl Acad Sci USA 2008, 105:4951-4956. 10.1073/pnas.0708743105, 2290808, 18353984.
32. Luo YC, Zhou H, Li Y, Chen JY, Yang JH, Chen YQ, Qu LH. Rice embryogenic calli express a unique set of microRNAs, suggesting regulatory roles of microRNAs in plant post-embryogenic development. FEBS Lett 2006, 580:5111-5116. 10.1016/j.febslet.2006.08.046, 16959252.
33. German MA, Pillay M, Jeong DH, Hetawal A, Luo SJ, Janardhanan P, Kannan V, Rymarquis LA, Nobuta K, German R, German R, De Paoli E, Lu C, Schroth G, Meyers BC, Green PJ. Global identification of microRNA-target RNA pairs by parallel analysis of RNA ends. Nat Biotechnol 2008, 26:941-946. 10.1038/nbt1417, 18542052.
34. Addo-Quaye C, Snyder JA, Park YB, Li YF, Sunkar R, Axtell MJ. Sliced microRNA targets and precise loop-first processing of MIR319 hairpins revealed by analysis of the Physcomitrella patens degradome. RNA 2009, 15:2112-2121. 10.1261/rna.1774909, 2779683, 19850910.
35. Addo-Quaye C, Eshoo TW, Bartel DP, Axtell MJ. Endogenous siRNA and miRNA targets identified by sequencing of the Arabidopsis degradome. Curr Biol 2008, 18:758-762. 10.1016/j.cub.2008.04.042, 2583427, 18472421.
36. Wu L, Zhang QQ, Zhou HY, Ni FR, Wu XY, Qi YJ. Rice microRNA effector complexes and targets. Plant Cell 2009, 21:3421-3435. 10.1105/tpc.109.070938, 2798332, 19903869.
37. Li YF, Zheng Y, Addo-Quaye C, Zhang L, Saini A, Jagadeeswaran G, Axtell MJ, Zhang WX, Sunkar R. Transcriptome-wide identification of microRNA targets in rice. Plant J 2010, 62:742-759. 10.1111/j.1365-313X.2010.04187.x, 20202174.
38. Yang JH, Li JH, Shao P, Zhou H, Chen YQ, Qu LH. starBase: a database for exploring microRNA-mRNA interaction maps from Argonaute CLIP-Seq and Degradome-Seq data. Nucleic Acids Res 2010, 39:D202-209. 3013664, 21037263.
39. Carthew RW, Sontheimer EJ. Origins and mechanisms of miRNAs and siRNAs. Cell 2009, 136:642-655. 10.1016/j.cell.2009.01.035, 2675692, 19239886.
40. Hamilton A, Voinnet O, Chappell L, Baulcombe D. Two classes of short interfering RNA in RNA silencing. EMBO J 2002, 21:4671-4679. 10.1093/emboj/cdf464, 125409, 12198169.
41. Chen X. Small RNAs and their roles in plant development. Annu Rev Cell Dev Biol 2009, 25:21-44. 10.1146/annurev.cellbio.042308.113417, 19575669.
42. Zilberman D, Cao X, Jacobsen SE. ARGONAUTE4 control of locus-specific siRNA accumulation and DNA and histone methylation. Science 2003, 299:716-719. 10.1126/science.1079695, 12522258.
43. Chan SW, Zilberman D, Xie Z, Johansen LK, Carrington JC, Jacobsen SE. RNA silencing genes control de novo DNA methylation. Science 2004, 303:1336. 10.1126/science.1095989, 14988555.
44. Wu L, Zhou HY, Zhang QQ, Zhang JG, Ni FR, Liu C, Qi YJ. DNA methylation mediated by a microRNA pathway. Mol Cell 2010, 38:465-475. 10.1016/j.molcel.2010.03.008, 20381393.
45. Wang X, Elling AA, Li X, Li N, Peng Z, He G, Sun H, Qi Y, Liu XS, Deng XW. Genome-wide and organ-specific landscapes of epigenetic modifications and their relationships to mRNA and small RNA transcriptomes in maize. Plant Cell 2009, 21:1053-1069. 10.1105/tpc.109.065714, 2685623, 19376930.
46. Valoczi A, Varallyay E, Kauppinen S, Burgyan J, Havelda Z. Spatio-temporal accumulation of microRNAs is highly coordinated in developing plant tissues. Plant J 2006, 47:140-151. 10.1111/j.1365-313X.2006.02766.x, 16824182.
47. Sieber P, Wellmer F, Gheyselinck J, Riechmann JL, Meyerowitz EM. Redundancy and specialization among plant microRNAs: role of the MIR164 family in developmental robustness. Development 2007, 134:1051-1060. 10.1242/dev.02817, 17287247.
48. Zhang BH, Pan XP, Cannon CH, Cobb GP, Anderson TA. Conservation and divergence of plant microRNA genes. Plant J 2006, 46:243-259. 10.1111/j.1365-313X.2006.02697.x, 16623887.
49. Zhang BH, Pan XP, Anderson TA. Identification of 188 conserved maize microRNAs and their targets. FEBS Lett 2006, 580:3753-3762. 10.1016/j.febslet.2006.05.063, 16780841.
50. Morin RD, Aksay G, Dolgosheina E, Ebhardt HA, Magrini V, Mardis ER, Sahinalp SC, Unrau PJ. Comparative analysis of the small RNA transcriptomes of Pinus contorta and Oryza sativa. Genome Res 2008, 18:571-584. 10.1101/gr.6897308, 2279245, 18323537.
51. Graham LK, Wilcox LW. The origin of alternation of generations in land plants: a focus on matrotrophy and hexose transport. Philos Trans R Soc Lond B Biol Sci 2000, 355:757-766. discussion 766-757, 10.1098/rstb.2000.0614, 1692790, 10905608.
52. Tang F. Small RNAs in mammalian germline: Tiny for immortal. Differentiation 2010, 79:141-146. 10.1016/j.diff.2009.11.002, 20227007.
53. Honys D, Twell D. Transcriptome analysis of haploid male gametophyte development in Arabidopsis. Genome Biol 2004, 5:R85. 10.1186/gb-2004-5-11-r85, 545776, 15535861.
54. Achard P, Herr A, Baulcombe DC, Harberd NP. Modulation of floral development by a gibberellin-regulated microRNA. Development 2004, 131:3357-3365. 10.1242/dev.01206, 15226253.
55. Millar AA, Gubler F. The Arabidopsis GAMYB-like genes, MYB33 and MYB65, are microRNA-regulated genes that redundantly facilitate anther development. Plant Cell 2005, 17:705-721. 10.1105/tpc.104.027920, 1069693, 15722475.
56. Wu MF, Tian Q, Reed JW. Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction. Development 2006, 133:4211-4218. 10.1242/dev.02602, 17021043.
57. Guo HS, Xie Q, Fei JF, Chua NH. MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for Arabidopsis lateral root development. Plant Cell 2005, 17:1376-1386. 10.1105/tpc.105.030841, 1091761, 15829603.
58. Jones-Rhoades MW, Bartel DP. Computational identification of plant microRNAs and their targets, including a stress-induced miRNA. Mol Cell 2004, 14:787-799. 10.1016/j.molcel.2004.05.027, 15200956.
59. Cecchetti V, Altamura MM, Falasca G, Costantino P, Cardarelli M. Auxin regulates Arabidopsis anther dehiscence, pollen maturation, and filament elongation. Plant Cell 2008, 20:1760-1774. 10.1105/tpc.107.057570, 2518247, 18628351.
60. de Martinis D, Cotti G, Hekker ST, Harren FJM, Mariani C. Ethylene response to pollen tube growth in Nicotiana tabacum flowers. Planta 2002, 214:806-812. 10.1007/s00425-001-0684-2, 11882951.
61. Huang S, Cerny RE, Qi YL, Bhat D, Aydt CM, Hanson DD, Malloy KP, Ness LA. Transgenic studies on the involvement of cytokinin and gibberellin in male development. Plant Physiol 2003, 131:1270-1282. 10.1104/pp.102.018598, 166887, 12644677.
62. Park JH, Halitschke R, Kim HB, Baldwin IT, Feldmann KA, Feyereisen R. A knock-out mutation in allene oxide synthase results in male sterility and defective wound signal transduction in Arabidopsis due to a block in jasmonic acid biosynthesis. Plant J 2002, 31:1-12. 10.1046/j.1365-313X.2002.01328.x, 12100478.
63. Cheng H, Song SS, Xiao LT, Soo HM, Cheng ZW, Xie DX, Peng JR. Gibberellin acts through jasmonate to control the expression of MYB21, MYB24, and MYB57 to promote stamen filament growth in Arabidopsis. PLoS Genet 2009, 5:e1000440. 10.1371/journal.pgen.1000440, 2654962, 19325888.
64. Baker SM, Bronner CE, Zhang L, Plug AW, Robatzek M, Warren G, Elliott EA, Yu JA, Ashley T, Arnheim N, Flavell RA, Liskay RM. Male-mice defective in the DNA mismatch repair gene Pms2 exhibit abnormal chromosome synapsis in meiosis. Cell 1995, 82:309-319. 10.1016/0092-8674(95)90318-6, 7628019.
65. Komori T, Ohta S, Murai N, Takakura Y, Kuraya Y, Suzuki S, Hiei Y, Imaseki H, Nitta N. Map-based cloning of a fertility restorer gene, Rf-1, in rice (Oryza sativa L.). Plant J 2004, 37:315-325.
66. Saze H, Kakutani T. Heritable epigenetic mutation of a transposon-flanked Arabidopsis gene due to lack of the chromatin-remodeling factor DDM1. EMBO J 2007, 26:3641-3652. 10.1038/sj.emboj.7601788, 1949009, 17627280.
67. O'Toole N, Hattori M, Andres C, Iida K, Lurin C, Schmitz-Linneweber C, Sugita M, Small I. On the expansion of the pentatricopeptide repeat gene family in plants. Mol Biol Evol 2008, 25:1120-1128. 10.1093/molbev/msn057, 18343892.
68. Le Trionnaire G, Twell D. Small RNAs in angiosperm gametophytes: from epigenetics to gamete development. Genes Dev 2010, 24:1081-1085. 10.1101/gad.1936110, 2878646, 20516193.
69. Cartagena JA, Matsunaga S, Seki M, Kurihara D, Yokoyama M, Shinozaki K, Fujimoto S, Azumi Y, Uchiyama S, Fukui K. The Arabidopsis SDG4 contributes to the regulation of pollen tube growth by methylation of histone H3 lysines 4 and 36 in mature pollen. Dev Biol 2008, 315:355-368. 10.1016/j.ydbio.2007.12.016, 18252252.
70. Ito M, Koike A, Koizumi N, Sano H. Methylated DNA-binding proteins from arabidopsis. Plant Physiol 2003, 133:1747-1754. 10.1104/pp.103.026708, 300729, 14605234.
71. Zemach A, McDaniel IE, Silva P, Zilberman D. Genome-wide evolutionary analysis of eukaryotic DNA methylation. Science 2010, 328:916-919. 10.1126/science.1186366, 20395474.
72. Lee TF, Zhai JX, Meyers BC. Conservation and divergence in eukaryotic DNA methylation. Proc Natl Acad Sci USA 2010, 107:9027-9028. 10.1073/pnas.1005440107, 2889049, 20457928.
73. Zemach A, Grafi G. Characterization of Arabidopsis thaliana methyl-CpG-binding domain (MBD) proteins. Plant J 2003, 34:565-572. 10.1046/j.1365-313X.2003.01756.x, 12787239.
74. Rousseaux S, Caron C, Govin K, Lestrat C, Faure AK, Khochbin S. Establishment of male-specific epigenetic information. Gene 2005, 345:139-153. 10.1016/j.gene.2004.12.004, 15716030.
75. Trasler JM. Epigenetics in spermatogenesis. Mol Cell Endocrinol 2009, 306:33-36. 10.1016/j.mce.2008.12.018, 19481683.
76. Liu SP, Li D, Li QB, Zhao P, Xiang ZH, Xia QY. MicroRNAs of Bombyx mori identified by Solexa sequencing. BMC Genomics 2010, 11:148. 10.1186/1471-2164-11-148, 2838851, 20199675.
77. ArrayExpress. , http://www.ebi.ac.uk/arrayexpress/
78. Qi X, Bao FS, Xie Z. Small RNA deep sequencing reveals role for Arabidopsis thaliana RNA-dependent RNA polymerases in viral siRNA biogenesis. PLoS One 2009, 4:e4971. 10.1371/journal.pone.0004971, 2654919, 19308254.
79. Gardner PP, Daub J, Tate JG, Nawrocki EP, Kolbe DL, Lindgreen S, Wilkinson AC, Finn RD, Griffiths-Jones S, Eddy SR, Bateman A. Rfam: updates to the RNA families database. Nucleic Acids Res 2009, 37:D136-D140. 10.1093/nar/gkn766, 2686503, 18953034.
80. Griffiths-Jones S, Bateman A, Marshall M, Khanna A, Eddy SR. Rfam: an RNA family database. Nucleic Acids Res 2003, 31:439-441. 10.1093/nar/gkg006, 165453, 12520045.
81. Griffiths-Jones S, Moxon S, Marshall M, Khanna A, Sr E, Bateman A. Rfam: annotating non-coding RNAs in complete genomes. Nucleic Acids Res 2005, 33:D121-D124. 540035, 15608160.
82. GenBank. , http://www.ncbi.nlm.nih.gov/genbank/
83. Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH, Nguyen JT, Barbisin M, Xu NL, Mahuvakar VR, Andersen MR, Lao KQ, Livak KJ, Guegler KJ. Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res 2005, 33:e179. 10.1093/nar/gni178, 1292995, 16314309.
84. Wang Q, Huang Z, Xue HL, Jin CC, Ju XL, Han JDJ, Chen YG. MicroRNA miR-24 inhibits erythropoiesis by targeting activin type I receptor ALK4. Blood 2008, 111:588-595. 10.1182/blood-2007-05-092718, 17906079.
85. Bu Y, Lu CH, Bian CJ, Wang JH, Li J, Zhang B, Li ZY, Brewer G, Zhao RC. Knockdown of Dicer in MCF-7 human breast carcinoma cells results in G1 arrest and increased sensitivity to cisplatin. Oncol Rep 2009, 21:13-17.
86. Varkonyi-Gasic E, Wu RM, Wood M, Walton EF, Hellens RP. Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs. Plant Methods 2007, 3:12. 10.1186/1746-4811-3-12, 2225395, 17931426.
87. Lacombe S, Nagasaki H, Santi C, Duval D, Piegu B, Bangratz M, Breitler J-C, Guiderdoni E, Brugidou C, Hirsch J, Cao X, Brice C, Panaud O, Karlowski WM, Sato Y, Echeverria M. Identification of precursor transcripts for 6 novel miRNAs expands the diversity on the genomic organisation and expression of miRNA genes in rice. BMC Plant Biol 2008, 8:123. 10.1186/1471-2229-8-123, 2607281, 19055717.