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Volumn 133, Issue 24, 2011, Pages 9545-9555

Complete budding and asymmetric division of primitive model cells to produce daughter vesicles with different interior and membrane compositions

Author keywords

[No Author keywords available]

Indexed keywords

AQUEOUS PHASE; AQUEOUS TWO PHASE SYSTEM; ASYMMETRIC FISSION; ASYMMETRIC-CELLS; CELL DIVISIONS; COMPLEX BEHAVIOR; GIANT LIPID VESICLES; LIPID COMPOSITION; LIPID NANOTUBES; MAMMALIAN CELLS; MEMBRANE COMPOSITION; MEMBRANE DOMAIN; MICROMETER-SCALE; MODEL SYSTEM; MOLE RATIO; MOLECULAR ASSEMBLY; OSMOLALITY; PEGYLATED LIPIDS; PRIMITIVE MODEL; PROTEIN CONCENTRATIONS; SELF-ASSEMBLED; SURFACE AREA; UNICELLULAR ORGANISMS;

EID: 79959187028     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja202406v     Document Type: Article
Times cited : (139)

References (120)
  • 77
    • 79959208564 scopus 로고    scopus 로고
    • Because of some variation in the specific polymer compositions encapsulated by each vesicle, (16) we see some variability in partitioning as well as in the osmolality required for budding and fission for individual vesicles even within a single batch. (25, 28)
    • Because of some variation in the specific polymer compositions encapsulated by each vesicle, (16) we see some variability in partitioning as well as in the osmolality required for budding and fission for individual vesicles even within a single batch. (25, 28)
  • 78
    • 79959204783 scopus 로고    scopus 로고
    • The osmolality of the PEG-rich phase was 115 ± 2.9 (50) when freshly prepared and increased to 136 ± 1.2 after incubation under parafilm at 42 °C for 2-3 days to form vesicles; thus even without addition of sucrose the external solution was hypertonic with respect to the solution encapsulated within the vesicles
    • The osmolality of the PEG-rich phase was 115 ± 2.9 (50) when freshly prepared and increased to 136 ± 1.2 after incubation under parafilm at 42 °C for 2-3 days to form vesicles; thus even without addition of sucrose the external solution was hypertonic with respect to the solution encapsulated within the vesicles.
  • 79
    • 79959253706 scopus 로고    scopus 로고
    • Vapor pressure osmometry measurements were performed at room temperature (25 °C). For the PEG-rich phase of the ATPS, measurements at this temperature will overestimate the osmolality under our experimental conditions (4 °C) by on the order of 10% or more; (51) thus, these values are most useful in a comparative rather than absolute sense
    • Vapor pressure osmometry measurements were performed at room temperature (25 °C). For the PEG-rich phase of the ATPS, measurements at this temperature will overestimate the osmolality under our experimental conditions (4 °C) by on the order of 10% or more; (51) thus, these values are most useful in a comparative rather than absolute sense.
  • 81
    • 79959209109 scopus 로고    scopus 로고
    • The initial addition of 130 mM sucrose led to a decrease in measured osmotic pressure, due to the nonadditive effect of multiple solutes on osmolality. (53) This did not alter the budded geometry of the vesicles, presumably because the total interior polymer concentration was on average lower in the ATPS from which the vesicles were formed. (16) Further aliquots of sucrose at increasing concentrations led to increased osmolality and the observed morphological changes leading to fission
    • The initial addition of 130 mM sucrose led to a decrease in measured osmotic pressure, due to the nonadditive effect of multiple solutes on osmolality. (53) This did not alter the budded geometry of the vesicles, presumably because the total interior polymer concentration was on average lower in the ATPS from which the vesicles were formed. (16) Further aliquots of sucrose at increasing concentrations led to increased osmolality and the observed morphological changes leading to fission.
  • 108
    • 79959244674 scopus 로고    scopus 로고
    • o lipid is visible between the PEG-rich and dextran-rich ends of the mother vesicle. We see membrane accumulation between the two aqueous phases under hypertonic conditions for some vesicles (28) and cannot be sure whether this indicates a barrier to diffusion between the two aqueous domains. Work by Li and Dimova suggests that this material is lipid nanotubes, (61) which would not seal off the two aqueous compartments. Our data do not enable us to make a distinction for this vesicle
    • o lipid is visible between the PEG-rich and dextran-rich ends of the mother vesicle. We see membrane accumulation between the two aqueous phases under hypertonic conditions for some vesicles (28) and cannot be sure whether this indicates a barrier to diffusion between the two aqueous domains. Work by Li and Dimova suggests that this material is lipid nanotubes, (61) which would not seal off the two aqueous compartments. Our data do not enable us to make a distinction for this vesicle.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.