Influence of intracellular trehalose concentration and pre-freeze cell volume on the cryosurvival of rapidly frozen human erythrocytes

Cryobiology

Volumn 63, Issue 1, 2011, Pages 26-31

  Lynch, Andrew L ^a   Slater, Nigel K H ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Biopolymer; Cell volume; Cryopreservation; Erythrocyte; Trehalose

Indexed keywords

POLYMER; PP 50; TREHALOSE; UNCLASSIFIED DRUG;

ARTICLE; CELL MEMBRANE PERMEABILITY; CELL TRANSPORT; CELL VOLUME; CONCENTRATION RESPONSE; CONTROLLED STUDY; CRYOPRESERVATION; ERYTHROCYTE LEVEL; ERYTHROCYTE LIFESPAN; ERYTHROCYTE PRESERVATION; HEMOLYSIS; HUMAN; HUMAN CELL; INCUBATION TIME; OSMOTIC STRESS; PRIORITY JOURNAL;

BLOOD PRESERVATION; CELL SIZE; CELL SURVIVAL; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; ERYTHROCYTES; HUMANS; TREHALOSE;

MAMMALIA;

EID: 79958862491     PISSN: 00112240     EISSN: 10902392     Source Type: Journal    
DOI: 10.1016/j.cryobiol.2011.04.005     Document Type: Article

References (26)

1. Acker J.P. The use of intracellular protectants in cell biopreservation. Advances in Biopreservation 2006, 291-313. Taylor and Francis, New York. J.G. Baust, J.M. Baust (Eds.).
2. Acker J.P., Lu X., Young V., Cheley S., Bayley H., Fowler A., Toner M. Measurement of trehalose loading of mammalian cells porated with a metal-actuated switchable pore. Biotechnol. Bioeng. 2003, 82:525-532.
3. Bhowmick P., Eroglu A., Wright D.L., Toner M., Toth T.L. Osmometric behavior of mouse oocytes in the presence of different intracellular sugars. Cryobiology 2002, 45:183-187.
4. Brahm J. Diffusional water permeability of human erythrocytes and their ghosts. J. Gen. Physiol. 1982, 79:791-819.
5. Chen T., Acker J.P., Eroglu A., Cheley S., Bayley H., Fowler A., Toner M. Beneficial effect of intracellular trehalose on the membrane integrity of dried mammalian cells. Cryobiology 2001, 43:168-181.
6. Crowe J.H., Crowe L.M., Oliver A.E., Tsvetkova N., Wolkers W., Tablin F. The trehalose myth revisited: introduction to a symposium on stabilization of cells in the dry state. Cryobiology 2001, 43:89-105.
7. Dash S.N., Routray P., Dash C., Guru B.C., Swain P., Sarangi N. Use of the non-toxic cryoprotectant trehalose enhances recovery and function of fish embryonic stem cells following cryogenic storage. Curr. Stem Cell Res. Ther. 2008, 3:277-287.
8. Eroglu A., Russo M.J., Bieganski R., Fowler A., Cheley S., Bayley H., Toner M. Intracellular trehalose improves the survival of cryopreserved mammalian cells. Nat. Biotechnol. 2000, 18:163-167.
9. Greenleaf J.E., Convertino V.A., Mangseth G.R. Plasma volume during stress in man: osmolality and red cell volume. J. Appl. Physiol. 1979, 47:1031-1038.
10. Hess J. An update on solutions for red cell storage. Vox Sang. 2006, 91:13-19.
11. Holovati J.L., Gyongyossy-Issa M.I.C., Acker J.P. Effects of trehalose-loaded liposomes on red blood cell response to freezing and post-thaw membrane quality. Cryobiology 2009, 58:75-83.
12. Lovelock J.E. Haemolysis by thermal shock. Brit. J. Haematol. 1955, 1:117.
13. Lynch A.L., Chen R., Dominowski P.J., Shalaev E.Y., Yancey R.J., Slater N.K.H. Biopolymer mediated trehalose uptake for enhanced erythrocyte cryosurvival. Biomaterials 2010, 31:6096-6103.
14. Lynch A.L., Chen R., Slater N.K.H. pH-responsive polymers for trehalose loading and desiccation protection of human red blood cells. Biomaterials 2011, 32:4443-4449.
15. Mazur P. Kinetics of water loss from cells at subzero temperatures and the likelihood of intracellular freezing. J. Gen. Physiol. 1963, 47:347-369.
16. Mazur P. Freezing of living cells: mechanisms and implications. Am. J. Physiol.-Cell Ph. 1984, 247:C125-C142.
17. Meryman H.T. Modified model for the mechanism of freezing injury in erythrocytes. Nature 1968, 218:333-336.
18. Meryman H.T. Osmotic stress as a mechanism of freezing injury. Cryobiology 1971, 8:489-500.
19. Pegg D.E., Diaper M.P. On the mechanism of injury to slowly frozen erythrocytes. Biophys. J. 1988, 54:471-488.
20. Rodrigues J.P., Paraguassú-Braga F.H., Carvalho L., Abdelhay E., Bouzas L.F., Porto L.C. Evaluation of trehalose and sucrose as cryoprotectants for hematopoietic stem cells of umbilical cord blood. Cryobiology 2008, 56:144-151.
21. Satpathy G.R., Török Z., Bali R., Dwyre D.M., Little E., Walker N.J., Tablin F., Crowe J.H., Tsvetkova N.M. Loading red blood cells with trehalose: a step towards biostabilization. Cryobiology 2004, 49:123-136.
22. Scott K., Ross-Rodriguez L.U., McGann L.E., Acker J.P. Alternative cryoprotective mechanism for intracellular trehalose: theoretical validation. Cryobiology 2004, 49:304.
23. Scott K.L., Lecak J., Acker J.P. Biopreservation of red blood cells: past, present, and future. Trans. Med. Rev. 2005, 19:127-142.
24. Williams R.J., Shaw S.K. The relationship between cell injury and osmotic volume reduction: II. Red cell lysis correlates with cell volume rather than intracellular salt concentration. Cryobiology 1980, 17:530-539.
25. Zhou X., He H., Liu B., Hua T. Loading trehalose into red blood cells by improved hypotonic method. Cell Preserv. Technol. 2008, 6:119-124.
26. Zhou X., Yuan J., Liu J., Liu B. Loading trehalose into red blood cells by electroporation and its application in freeze-drying. Cryoletters 2010, 31:147-156.