Comparison of high performance liquid chromatography, radio immunoassay and electrochemiluminescence immunoassay for quantification of serum 25 hydroxy vitamin D

Clinical Biochemistry

Volumn 44, Issue 10-11, 2011, Pages 864-868

  Jafri, Lena ^a   Khan, Aysha Habib ^a   Siddiqui, Anwar A ^a   Mushtaq, Shamim ^b   Iqbal, Romaina ^a   Ghani, Farooq ^a   Siddiqui, Imran ^a  

^a AGA KHAN UNIVERSITY   (Pakistan)

^b UNIVERSITY OF KARACHI   (Pakistan)

Author keywords

High performance liquid chromatography; Methodology; Radio immunoassay; Vitamin D

Indexed keywords

25 HYDROXYVITAMIN D;

ARTICLE; BLOOD LEVEL; CHEMOLUMINESCENCE; COMPARATIVE STUDY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; ELECTROCHEMICAL ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; INTERMETHOD COMPARISON; PRIORITY JOURNAL; RADIOIMMUNOASSAY;

CHEMILUMINESCENT MEASUREMENTS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROCHEMICAL TECHNIQUES; HUMANS; RADIOIMMUNOASSAY; VITAMIN D;

EID: 79958851298     PISSN: 00099120     EISSN: 18732933     Source Type: Journal    
DOI: 10.1016/j.clinbiochem.2011.04.020     Document Type: Article

References (22)

1. Holick M.F. High prevalence of vitamin D inadequacy and implications for health. Mayo Clin Proc. 2006 Mar, 81(3):353-373.
2. Holick M.F. Vitamin D: a D-Lightful health perspective. Nutr Rev 2008 Oct, 66:S182-S194. 10 Suppl 2.
3. Souberbielle J.C., Prie D., Courbebaisse M., Friedlander G., Houillier P., Maruani G., et al. Update on vitamin D and evaluation of vitamin D status. Ann Endocrinol (Paris). 2008 Dec, 69(6):501-510.
4. Holick M.F., Vitamin D. Deficiency. N Engl J Med 2007, 357:266-281.
5. Inoue Y., Kaji H., Hisa I., Tobimatsu T., Naito J., Iu M.F., et al. Vitamin D status affects osteopenia in postmenopausal patients with primary hyperparathyroidism. Endocr J. 2008 Mar, 55(1):57-65.
6. Masuda S. Vitamin D: its chemistry, metabolism and biological functions. Nippon Rinsho. 1999 Oct, 57(10):2230-2235.
7. Hollis B.W. Measuring 25-hydroxyvitamin D in a clinical environment: challenges and needs. Am J Clin Nutr. 2008 Aug, 88(2):507S-510S.
8. Hollis B.W. Editorial: the determination of circulating 25-hydroxyvitamin D: no easy task. J Clin Endocrinol Metab. 2004 Jul, 89(7):3149-3151.
9. Vieth R. Problems with direct 25-hydroxyvitamin D assays, and the target amount of vitamin D nutrition desirable for patients with osteoporosis. Osteoporos Int. 2000, 11(7):635-636.
10. Bouillon R., Van Herck E., Jans I., Tan B.K., Van Baelen H., De Moor P. Two direct (nonchromatographic) assays for 25-hydroxyvitamin D. Clin Chem. 1984 Nov, 30(11):1731-1736.
11. Bikle D.D., Gee E., Halloran B., Kowalski M.A., Ryzen E., Haddad J.G. Assessment of the free fraction of 25-hydroxyvitamin D in serum and its regulation by albumin and the vitamin D-binding protein. J Clin Endocrinol Metab. 1986 Oct, 63(4):954-959.
12. Haddad J.G., Chyu K.J. Competitive protein-binding radioassay for 25-hydroxycholecalciferol. J Clin Endocrinol Metab. 1971 Dec, 33(6):992-995.
13. Jones G. Assay of vitamins D2 and D3, and 25-hydroxyvitamins D2 and D3 in human plasma by high-performance liquid chromatography. Clin Chem. 1978 Feb, 24(2):287-298.
14. Hollis B.W. Comparison of commercially available (125)I-based RIA methods for the determination of circulating 25-hydroxyvitamin D. Clin Chem. 2000 Oct, 46(10):1657-1661.
15. Habib A., Vitamin D. Deficiency: are we still ignoring the evidence?. J Pak Med Assoc 2008 Sep, 58(9):529.
16. Khan A.H., Iqbal R. Vitamin D deficiency in an ample sunlight country. J Coll Physicians Surg Pak. 2009 May, 19(5):267-268.
17. Atiq M., Suria A., Nizami S.Q., Ahmed I. Maternal vitamin-D deficiency in Pakistan. Acta Obstet Gynecol Scand. 1998 Nov, 77(10):970-973.
18. Mansoor S., Habib A., Ghani F., Fatmi Z., Badruddin S., Mansoor S., Siddiqui I. Jabbar. Prevalence and significance of Vitamin D deficiency and insufficiency among apparently healthy adults. Clinical Biochemistry. 2010, 43:1431-1435.
19. Zuberi L.M., Habib A., Haque N., Jabbar A. Vitamin D deficiency in ambulatory patients. J Pak Med Assoc. 2008 Sep, 58(9):482-484.
20. Glendenning P., Taranto M., Noble J.M., Musk A.A., Hammond C., Goldswain P.R., et al. Current assays overestimate 25-hydroxyvitamin D3 and underestimate 25-hydroxyvitamin D2 compared with HPLC: need for assay-specific decision limits and metabolite-specific assays. Ann Clin Biochem. 2006 Jan, 43(Pt 1):23-30.
21. Binkley N., Krueger D., Gemar D., Drezner M.K. Correlation among 25-hydroxy-vitamin D assays. J Clin Endocrinol Metab. 2008 May, 93(5):1804-1808.
22. Leino A., Turpeinen U., Koskinen P. Automated measurement of 25-OH vitamin D3 on the Roche Modular E170 analyzer. Clin Chem. 2008 Dec, 54(12):2059-2062.