Isolation and characterization of an antigen from the fish pathogen Moritella viscosa

Journal of Applied Microbiology

Volumn 111, Issue 1, 2011, Pages 17-25

  Bjornsson H ^a   Marteinsson, V P ^b   Fridjónsson, Ó H ^b   Linke, D ^c   Benediktsdottir E ^a  

^a UNIVERSITY OF ICELAND   (Iceland)

^b Matis ohf Iceland   (Iceland)

^c MAX PLANCK INSTITUTE FOR DEVELOPMENTAL BIOLOGY   (Germany)

Author keywords

Aquaculture; Membrane; Pathogenesis; Proteomics; Vaccines

Indexed keywords

ANTIGENS; BIOLOGICAL MEMBRANES; CLONING; ESCHERICHIA COLI; FISH; GENE ENCODING; MOLECULAR BIOLOGY; PROTEINS; STRAIN;

EXTRACELLULAR LOOPS; FARMED FISHES; ISOLATION AND CHARACTERIZATION; MORITELLA VISCOSA; OUTER MEMBRANE PROTEIN; PATHOGENESIS; PATHOGENICS; PROTEOMICS; PSYCHROPHILIC BACTERIA; WINTER ULCERS;

VACCINES;

BACTERIAL ANTIGEN; OUTER MEMBRANE PROTEIN;

ANTIGEN; BACTERIUM; FISH; FISH CULTURE; GENE EXPRESSION; HOMOLOGY; MEMBRANE; MOLECULAR ANALYSIS; PATHOGEN; PATHOGENICITY; PEPTIDE; PROTEIN; PROTEOMICS; VACCINE;

ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATION; ESCHERICHIA COLI; FISH; GENE EXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; MORITELLA; MORITELLA VISCOSA; NEISSERIA; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; STRAIN DIFFERENCE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MORITELLA VISCOSA; NEGIBACTERIA; NEISSERIA;

EID: 79958841709     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2011.05023.x     Document Type: Article

References (48)

1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J. (1990) Basic Local Alignment Search Tool. J Mol Biol 215, 403-410.
2. Arnold, T. and Linke, D. (2007) Phase separation in the isolation and purification of membrane proteins. BioTechniques 43, 427-440.
3. Bendtsen, J.D., Nielsen, H., von Heijne, G. and Brunak, S. (2004) Improved Prediction of Signal Peptides: SignalP 3.0. J Mol Biol 340, 783-795.
4. Benediktsdóttir, E. and Heidarsdóttir, K.J. (2007) Growth and lysis of the fish pathogen Moritella viscosa. Lett Appl Microbiol 45, 115-120.
5. Benediktsdóttir, E., Helgason, S. and Sigurjónsdóttir, H. (1998) Vibrio spp. isolated from salmonids with shallow skin lesions and reared at low temperature. J Fish Dis 21, 19-28.
6. Benediktsdóttir, E., Verdonck, L., Spröer, C., Helgason, S. and Swings, J. (2000) Characterization of Vibrio viscosus and Vibrio wodanis isolated at different geographical locations: a proposal for reclassification of Vibrio viscosus as Moritella viscosa comb. nov. Int J Syst Evol Microbiol 50, 479-488.
7. Biegert, A., Mayer, C., Remmert, M., Söding, J. and Lupas, A.N. (2006) The MPI bioinformatics toolkit for protein sequence analysis. Nucleic Acids Res 34(web server issue), W335-W339.
8. Birkbeck, T.H., Billcliffe, B., Laidler, A. and Cox, D.I. (2000) The relationship between Aeromonas sp. NCIMB 2263, a causative agent of skin lesions in Atlantic salmon, Vibrio marinus (Moritella marina) and Vibrio viscosus. J Fish Dis 23, 281-283.
9. Björnsdóttir, B., Fridjónsson, Ó.H., Magnúsdóttir, S., Andrésdóttir, V., Hreggvidsson, G.Ó. and Gudmundsdóttir, B.K. (2009a) Characterisation of an extracellular vibriolysin of the fish pathogen Moritella viscosa. Vet Microbiol 136, 326-334.
10. Björnsdóttir, B., Fast, M.D., Sperker, S., Brown, L.L. and Gudmundsdóttir, B.K. (2009b) Effects of Moritella viscosa antigens on pro-inflammatory gene expression in an Atlantic salmon (Salmo salar Linnaeus) cell line (SHK-1). Fish Shellfish Immunol 26, 858-863.
11. Bordier, C. (1981) Phase Separation of Integral Membrane Proteins in Triton X-114 solution. J Biol Chem 256, 1604-1607.
12. Bornø, G., Kvellestad, A., Olsen, A.B., Flesjå, K., Skjelstad, H., Mo, T.A. and Hjeltnes, B. (2006) Helsesituasjonen hos laksefisk 2005. In Helsesituasjonen hos oppdrettsfisk 2005, Report. pp. 3-12. Oslo, Norway: National Veterinary Institute ( ).
13. Bruno, D.W., Griffiths, J., Petrie, J. and Hastings, T.S. (1998) Vibrio viscosus in farmed Atlantic salmon Salmo salar in Scotland: field and experimental observations. Dis Aquat Org, 34, 161-166.
14. Callaghan, M.J., Buckee, C., McCarthy, N.D., Pavón, A.B.I., Jolley, K.A., Faust, S., Cray, S.J., Kaczmarski, E.B. et al. (2008) Opa protein repertoires of disease-causing and carried meningococci. J Clin Microbiol 46, 3033-3041.
15. Colquhoun, D.J., Hovland, H., Hellberg, H., Haug, T. and Nilsen, H. (2004) Moritella viscosa isolated from farmed Atlantic cod (Gadus morhua). Bull Eur Assoc Fish Pathol 24, 109-114.
16. Corpet, F. (1988) Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 16, 10881-10890.
17. Crump, E.M., Burian, J., Allen, P.D. and Key, W.W. (2005) Identification and expression of a host-recognized antigen, FspA, from Flavobacterium psychrophilum. Microbiology 151, 3127-3135.
18. Dass, C. (2007) Fundamentals of Contemporary Mass Spectrometry, 1st edn. pp. 289-337. New Jersey: Wiley-Interscience.
19. Dehio, C., Gray-Owen, S.D. and Meyer, T.F. (1998) The role of neisserial Opa proteins in interactions with host cells. Trends Microbiol 6, 489-495.
20. Dumetz, F., Duchaud, E., Claverol, S., Orieux, N., Papillon, S., Lapaillerie, D. and Hénaff, M.L. (2008) Analysis of the Flavobacterium psychrophilum outer-membrane subproteome and identification of new antigenic targets for vaccine by immunomics. Microbiology 154, 1793-1801.
21. Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud, S., Wilkins, M.R., Appel, R.D. and Bairoch, A. (2005) Protein Identification and Analysis Tools on the ExPASy Server. In The Proteomics Protocols Handbook ed. Walker, J.M. pp. 571-607. New York: Humana Press.
22. Grove, S., Reitan, L.J., Lunder, T. and Colquhoun, D. (2008) Real-Time PCR detection of Moritella viscosa, the likely causal agent of winter-ulcer in Atlantic salmon Salmo salar and rainbow trout Oncorhynchus mykiss. Dis Aquat Org 82, 105-109.
23. Grove, S., Wiik-Nielsen, C.R., Lunder, T., Tunsjø, H.S., Tandstad, N.M., Reitan, L.J., Marthinussen, A., Sørgaard, M. et al. (2010) Previously unrecognised division within Moritella viscosa isolated from fish farmed in the North Atlantic. Dis Aquat Org 93, 51-61.
24. Guex, N. and Peitsch, M.C. (1997) SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modelling. Electrophoresis 18, 14-23.
25. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95-98.
26. Heidarsdóttir, K.J., Gravningen, K. and Benediktsdóttir, E. (2008) Antigen profiles of the fish pathogen Moritella viscosa and protection in fish. J Appl Microbiol 104, 944-951.
27. Hellberg, H., Nilsen, H.K., Bornø, G., Skjelstad, H.R., Colquhoun, D. and Jensen, B.B. (2010) Helsesituasjonen hos marin fisk 2009. In Fiskehelserapporten 2009, Report. pp. 25-32. National Veterinary Institute. ( ).
28. Jackman, P.J.H. (1987) Microbial systematics based on electrophoretic whole-cell protein patterns. In Methods in Microbiology, Vol. 19 ed. Colwell, R.R. and Grigorova, R. pp. 209-225. London: Academic Press.
29. Kleinschmidt, J.H. (2003) Membrane protein folding on the example of outer membrane protein A of Escherichia coli. Cell Mol Life Sci 60, 1547-1558.
30. Koebnik, R. (1999) Structural and Functional Roles of the Surface-Exposed Loops of the β-Barrel Membrane Protein OmpA from Escherichia coli. J Bacteriol 181, 3688-3694.
31. Lunder, T. (1992) "Winter ulcer" in Atlantic salmon, a study of pathological changes, transmissibility and bacterial isolates. PhD Dissertation, The Norwegian Collage of Veterinary Medicine, Oslo.
32. Lunder, T., Sørum, H., Holstad, G., Steigerwalt, A.G., Mowinckel, P. and Brenner, D.J. (2000) Phenotypic and genotypic characterization of Vibrio viscosus sp. nov. and Vibrio wodanis sp. nov. isolated from Atlantic salmon (Salmo salar) with 'winter ulcer'. Int J Syst Evol Microbiol 50, 427-450.
33. Marchler-Bauer, A. and Bryant, S.H. (2004) CD-Search: protein domain annotations on the fly. Nucleic Acids Res 32, 327-331.
34. Martin, D., Cadieux, N., Hamel, J. and Brodeur, B.R. (1997) Highly Conserved Neisseria meningitidis Surface Protein Confers Protection against Experimental Infection. J Exp Med 185, 1173-1183.
35. Miroux, B. and Walker, J.E. (1996) Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J Mol Biol 260, 289-298.
36. Olsen, A.B., Bornø, G., Colquhoun, D., Flesjå, K., Haldorsen, R., Mo, T.A., Nilsen, H., Skjelstad, H.R. et al. (2007) Helsesituasjonen hos laksefisk i 2006. In Helsesituasjonen hos oppdrettsfisk 2006, Report. pp. 3-12. Oslo, Norway: National Veterinary Institute. ( ).
37. Sambrook, J. and Russell, D.W. (2001) Molecular cloning: A Laboratory Manual. 3rd edn, Vol. 1-3. New York: Cold Spring Harbor Laboratory Press.
38. Shevchenko, A., Wilm, M., Vorm, O. and Mann, M. (1996) Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels. Anal Chem 68, 850-858.
39. Söding, J. (2005) Protein homology detection by HMM-HMM comparison. Bioinformatics 21, 951-960.
40. Stumpp, T., Wilms, B. and Altenbuchner, J. (2000) Ein neues, L-rhamnose-induzierbares Expressionssystem für Escherichia coli. Biospektrum 6, 33-36.
41. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876-4882.
42. Tsai, C.-M. and Frasch, C.E. (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 119, 115-119.
43. Tunsjø, H.S. (2009) Moritella viscosa, the aetiological agent of winter ulcer: Interactions with host and environment. Thesis for the degree of Philosophiae Doctor, Norwegian School of Veterinary Science, Oslo.
44. Tunsjø, H.S., Paulsen, S.M., Mikkelsen, H., L'abée-Lund, T.M., Skjerve, E. and Sørum, H. (2007) Adaptive response to environmental changes in the fish pathogen Moritella viscosa. Res Microbiol 158, 244-250.
45. Tunsjø, H.S., Paulsen, S.M., Berg, K., Sørum, H. and L'Abée-Lund, T.M. (2009) The winter ulcer bacterium Moritella viscosa demonstrates adhesion and cytotoxicity in a fish cell model. Microb Pathog 47, 134-142.
46. Wang, Y. (2002) The Function of OmpA in Escherichia coli. Biochem Biophys Res 292, 396-401.
47. Whitman, K.A., Backman, S., Benediktsdóttir, E., Coles, M. and Johnson, G.R. (2000) Isolation and Characterization of a New Vibrio spp. (Vibrio wodanis) Associated with 'Winter Ulcer Disease' in Sea Water Raised Atlantic Salmon (Salmo salar L.) in New Brunswick. In Aquaculture Canada 2000-Proceedings of the 17th Annual Meeting of the Aquaculture Association of Canada, Moncton, 28-31 May 2000. Aquaculture Association of Canada Special publication no. 4, ed. Hendry, C.I. and McGladdery, W.E., pp. 115-117. St Andrews, Canada: Aquaculture Association of Canada.
48. Wilm, M., Shevchenko, A., Houthave, T., Breit, S., Schweigerer, L., Fotsis, T. and Mann, M. (1996) Femtomole sequencing of proteins from polyacrylamide gels by nano electrospray mass spectrometry. Nature 379, 466-469.