Reduced activity and cytoplasmic localization of Runx2 is observed in C3h10t1/2 cells overexpressing Tbx3

Cell Biochemistry and Function

Volumn 29, Issue 4, 2011, Pages 348-350

  Deepak, Vishwa ^a   Zhang, Zhongli ^a   Meng, Lingyi ^a   Zeng, Xianlu ^a   Liu, Wenguang ^a  

^a NORTHEAST NORMAL UNIVERSITY   (China)

Author keywords

C3h10t1 2; Osteoblast; Osteopontin; Runx2; Tbx3

Indexed keywords

OSTEOPONTIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR RUNX2; TRANSCRIPTION FACTOR TBX3; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL NUCLEUS; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; GENE OVEREXPRESSION; GENETIC TRANSFECTION; IMMUNOFLUORESCENCE; MOUSE; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; CELL LINE; CELL NUCLEUS; CORE BINDING FACTOR ALPHA 1 SUBUNIT; CYTOPLASM; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION REGULATION; LUCIFERASES; MICE; OSTEOPONTIN; PROMOTER REGIONS, GENETIC; T-BOX DOMAIN PROTEINS; TRANSFECTION;

EID: 79958826616     PISSN: 02636484     EISSN: 10990844     Source Type: Journal    
DOI: 10.1002/cbf.1753     Document Type: Article

References (17)

1. Smith J. T-box genes: what they do and how they do it. Trends Genet 1999; 15: 154-158.
2. Bamshad M, Lin RC, Law DJ, et al. Mutations in human TBX3 alter limb, apocrine and genital development in ulnar-mammary syndrome. Nat Genet 1997; 16: 311-315.
3. Klopocki E, Neumann LM, Tonnies H, Ropers HH, Mundlos S, Ullmann R. Ulnar-mammary syndrome with dysmorphic facies and mental retardation caused by a novel 1.28Mb deletion encompassing the TBX3 gene. Eur J Hum Genet 2006; 14: 1274-1279.
4. Govoni KE, Lee SK, Chadwick RB, et al. Whole genome microarray analysis of growth hormone-induced gene expression in bone: T-box3, a novel transcription factor, regulates osteoblast proliferation. Am J Physiol Endocrinol Metab 2006; 291: E128-E136.
5. Govoni KE, Linares GR, Chen ST, Pourteymoor S, Mohan S. T-box 3 negatively regulates osteoblast differentiation by inhibiting expression of osterix and runx2. J Cell Biochem 2009; 106: 482-490.
6. Ducy P, Zhang R, Geoffroy V, Ridall AL, Karsenty G. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell 1997; 89: 747-754.
7. Ogawa E, Maruyama M, Kagoshima H, et al. PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene. Proc Natl Acad Sci USA 1993; 90: 6859-6863.
8. Komori T, Yagi H, Nomura S, et al. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 1997; 89: 755-764.
9. Mundlos S, Otto F, Mundlos C, et al. Mutations involving the transcription factor CBFA1 cause cleidocranial dysplasia. Cell 1997; 89: 773-779.
10. Liu W, Toyosawa S, Furuichi T, et al. Overexpression of Cbfa1 in osteoblasts inhibits osteoblast maturation and causes osteopenia with multiple fractures. J Cell Biol 2001; 155: 157-166.
11. Morita S, Kojima T, Kitamura T. Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther 2000; 7: 1063-1066.
12. Carlson H, Ota S, Campbell CE, Hurlin PJ. A dominant repression domain in Tbx3 mediates transcriptional repression and cell immortalization: relevance to mutations in Tbx3 that cause ulnar-mammary syndrome. Hum Mol Genet 2001; 10: 2403-2413.
13. Harada H, Tagashira S, Fujiwara M, et al. Cbfa1 isoforms exert functional differences in osteoblast differentiation. J Biol Chem 1999; 274: 6972-6978.
14. Fujita T, Azuma Y, Fukuyama R, et al. Runx2 induces osteoblast and chondrocyte differentiation and enhances their migration by coupling with PI3K-Akt signaling. J Cell Biol 2004; 166: 85-95.
15. Zaidi SK, Javed A, Choi JY, et al. A specific targeting signal directs Runx2/Cbfa1 to subnuclear domains and contributes to transactivation of the osteocalcin gene. J Cell Sci 2001; 114: 3093-3102.
16. Fujita T, Izumo N, Fukuyama R, et al. Phosphate provides an extracellular signal that drives nuclear export of Runx2/Cbfa1 in bone cells. Biochem Biophys Res Commun 2001; 280: 348-352.
17. Pockwinse SM, Rajgopal A, Young DW, et al. Microtubule-dependent nuclear-cytoplasmic shuttling of Runx2. J Cell Physiol 2006; 206: 354-362.