Functional identification of hyoscyamine 6β-hydroxylase from Anisodus acutangulus and overproduction of scopolamine in genetically-engineered Escherichia coli

Biotechnology Letters

Volumn 33, Issue 7, 2011, Pages 1361-1365

  Kai, Guoyin ^a   Liu, Yuanyuan ^a   Wang, Xiaoyun ^b   Yang, Sheng ^a   Fu, Xueqing ^a   Luo, Xiuqin ^a   Liao, Pan ^a  

^a SHANGHAI NORMAL UNIVERSITY   (China)

^b Kunshan Hospital of Traditional Chinese Medicine   (China)

Author keywords

Anisodus acutangulus; Hyoscyamine; Hyoscyamine 6 hydroxylase; Scopolamine

Indexed keywords

ANISODUS ACUTANGULUS; BIOSYNTHETIC PATHWAY; BOLT ANALYSIS; FUNCTIONAL CHARACTERIZATION; FUNCTIONAL IDENTIFICATION; GENETIC IMPROVEMENTS; HIS-TAG; HYDROXYLASES; HYOSCYAMINE; N-TERMINALS; RECOMBINANT PROTEIN; SCOPOLAMINE;

GENE ENCODING; PROTEINS; PURIFICATION;

ESCHERICHIA COLI;

ATROPINE; HYOSCYAMINE (6S) DIOXYGENASE; HYOSCYAMINE (6S)-DIOXYGENASE; MIXED FUNCTION OXIDASE; RECOMBINANT PROTEIN; SCOPOLAMINE;

ANGIOSPERM; ARTICLE; BIOLOGICAL MODEL; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING;

ATROPINE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; METABOLIC NETWORKS AND PATHWAYS; MIXED FUNCTION OXYGENASES; MODELS, BIOLOGICAL; RECOMBINANT PROTEINS; SCOPOLAMINE; SOLANACEAE;

ACUTANGULUS; ANISODUS; ESCHERICHIA COLI;

EID: 79958765547     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0575-y     Document Type: Article

References (13)

1. Cardillo AB, Talou JR, Giulietti AM (2008) Expression of Brugmansia candida Hyoscyamine 6beta-Hydroxylase gene in Saccharomyces cerevisiae and its potential use as biocatalyst. Microb Cell Fact 7: 17.
2. Hashimoto T, Yamada Y (1987) Purification and characterization of hyoscyamine 6β-hydroxylase from root cultures of Hyoscyamus niger. Eur J Biochem 164(2): 277-285.
3. Hashimoto T, Matsuda J, Yamada Y (1993) Two-step epoxidation of hyoscyamine to scopolamine is catalyzed by bifunctional hyoscyamine 6β-hydroxylase. FEBS Lett 329(1-2): 35-39.
4. Jouhikainen K, Lindgren L, Jokelainen T, Hiltunen R, Teeri T, Oksman-Caldentey KM (1999) Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering. Planta 208: 545-551.
5. Kai GY, Chen JF, Li L, Zhou GY, Zhou LM, Zhang L, Chen YH, Zhao LX (2007) Molecular cloning and characterization of a new cDNA encoding hyoscyamine 6β-hydroxylase from root of Anisodus acutangulus. J Biochem Mol Biol 40(5): 715-722.
6. Kai GY, Zhang Y, Chen JF, Li L, Yan XM, Zhang R, Liao P, Lu X, Wang W, Zhou GY (2009a) Molecular characterization and expression analysis of two distinct putrescine N-methyltransferases from roots of Anisodus acutangulus. Physiol Plant 135(2): 121-129.
7. Kai GY, Li L, Jiang YX, Yan XM, Zhang Y, Lu X, Liao P, Chen JB (2009b) Molecular cloning, characterization of two tropinone reductases in Anisodus acutangulus and enhancement of tropane alkaloids production AaTRI transformed hairy roots. Biotechnol Appl Biochem 54(3): 177-186.
8. Liu T, Zhu P, Cheng KD, Meng C, He HX (2005) Molecular cloning, expression and characterization of hyoscyamine 6beta-hydroxylase from hairy roots of Anisodus tanguticus. Planta Med 71(3): 249-253.
9. Matsuda J, Okabe S, Hashimoto T, Yamada Y (1991) Molecular cloning of hyoscyamine 6β-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, from cultured roots of Hyoscyamus niger. J Biol Chem 266(15): 9460-9464.
10. Suzuki K, Yun DJ, Chen XY, Yamada Y, Hashimoto T (1999) An Atropa belladonna hyoscyamine 6-hydroxylase gene is differentially expressed in the root pericycle and anthers. Plant Mol Biol 40: 141-152.
11. Yun DJ, Hashimoto T, Yamada Y (1992) Metabolic engineering of medicinal plants: Transgenic Atropa belladonna with an improved alkaloid composition. Proc Natl Acad Sci USA 89(24): 11799-11803.
12. Zarate R, El Jaber-Vazdekis N, Medina B, Ravelo AG (2006) Tailoring tropane alkaloid accumulation in transgenic hairy roots of Atropa baetica by over-expressing the gene encoding hyoscyamine 6β-hydroxylase. Biotechnol Lett 28(16): 1271-1277.
13. Zhang L, Ding RX, Chai YR, Bonfill M, Piñol MT, Xu TF, Pi Y, Wang ZN, Zhang HM, Kai GY, Liao ZH, Sun XF, Tang K (2004) Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures. Proc Natl Acad Sci USA 101(17): 6786-6791.