PCR-DGGE analysis of bacterial community dynamics in kava beverages during refrigeration

Letters in Applied Microbiology

Volumn 53, Issue 1, 2011, Pages 30-34

  Dong, J ^a   Kandukuru, P ^a   Huang, A S ^a   Li, Y ^a  

^a UNIVERSITY OF HAWAII   (United States)

Author keywords

Bacterial community dynamics; Kava beverages; PCR DGGE; Species specific PCR

Indexed keywords

BEVERAGES; DYNAMICS; ELECTROPHORESIS; LABORATORIES; POLYMERASE CHAIN REACTION; REFRIGERATION; SPOILAGE;

AEROBIC PLATE COUNTS; BACTERIAL COMMUNITY; BACTERIAL COMMUNITY DYNAMIC; COMMUNITY DYNAMICS; KAVA BEVERAGE; LACTIC ACID BACTERIA; POLYMERASE CHAIN REACTION-DENATURING GRADIENT GEL ELECTROPHORESES (PCR-DGGE); SPECIES SPECIFICS; SPECIES-SPECIFIC PCR; YEAST AND MOLD COUNT;

LACTIC ACID;

BACTERIAL DNA; KAVA;

BACTERIUM; ELECTROKINESIS; MICROBIAL COMMUNITY; PH; POLYMERASE CHAIN REACTION; PRESERVATION; SAMPLING;

ARTICLE; BACTERIAL COUNT; BEVERAGE; COMMUNITY DYNAMICS; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA EXTRACTION; FOOD SPOILAGE; FREEZING; LACTIC ACID BACTERIUM; LACTOCOCCUS LACTIS; MICROBIAL COMMUNITY; MOULD; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS; PSEUDOMONAS PUTIDA; WEISSELLA; YEAST;

BACTERIA (MICROORGANISMS); LACTOBACILLUS; LACTOCOCCUS LACTIS; PIPER METHYSTICUM; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS; PSEUDOMONAS PUTIDA; WEISSELLA SOLI;

EID: 79958765243     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2011.03065.x     Document Type: Article

References (16)

1. Dogan, B. and Boor, K.J. (2003) Genetic diversity and spoilage potentials among Pseudomonas spp. isolated from fluid milk products and dairy processing plants. Appl Environ Microbiol 69, 130-138.
2. Ercolini, D., Russo, F., Torrieri, E., Masi, P. and Villani, F. (2006) Changes in the spoilage-related microbiota of beef during refrigerated storage under different packaging conditions. Appl Environ Microbiol 72, 4663-4671.
3. Ercolini, D., Russo, F., Blaiotta, G., Pepe, I., Mauriello, G. and Villani, F. (2007) Simultaneous detection of P. fragi, P. lundensis, and P. putida from meat by use of a multiplex PCR assay targeting the carA gene. Appl Environ Microbiol 73, 2354-2359.
4. Hilario, E., Buckley, T.R. and Young, J.M. (2004) Improved resolution of the phylogenetic relationships among Pseudomonas by the combined analysis of atpD, carA, recA and 16S rDNA. Antonie Van Leeuwenhoek 86, 51-64.
5. Jay, J.M. (2000) Modern Food Microbiology, pp. 11-34. Gaithersburg MD: Aspen Publishers.
6. Kandukuru, P., Huang, A.S., Dong, J., Bittenbender, H.C. and Li, Y. (2009) Rapid identification of bacterial isolates from aqueous kava (Piper methysticum) extracts by polymerase chain reaction and DNA sequencing. Lett Appl Microbiol 49, 764-768.
7. Kwan, K.K. and Skura, B.J. (1985) Identification of proteolytic Pseudomonads isolated from raw milk. J Dairy Sci 68, 1902-1909.
8. Labadie, J. (1999) Consequences of packaging on bacterial growth: meat is an ecological niche. Meat Sci 52, 299-305.
9. Lebot, V. and Simeoni, P. (2004) Is the quality of kava (Piper methysticum Forst. f.) responsible for different geographical patterns? Ethnobot Res Appl 2, 19-28.
10. Lebot, V., Merlin, M. and Lindstrom, L. (1997) Kava-The Pacific Elixir: The Definitive Guide to its Ethnobotany, History, and Chemistry, pp. 60-63. New Haven, CT: Yale University Press.
11. Muyzer, G., De Wall, E.C. and Uitterlinden, A.G. (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis of 16S ribosomal DNA fragments. Appl Environ Microbiol 59, 695-700.
12. Myers, R.M., Fischer, S.G., Lerman, L.S. and Maniatis, T. (1985) Nearly all single base substitutions in DNA fragments joined to a GC-clamp can be detected by denaturing gradient gel electrophoresis. Nucleic Acids Res 13, 3131-3145.
13. Ogier, J.C., Son, O., Gruss, A., Tailliez, P. and Delacroix-Buchet, A. (2002) Identification of the bacterial microflora in dairy products by temporal temperature gradient gel electrophoresis. Appl Environ Microbiol 68, 3691-3701.
14. Scarpellini, M., Franzetti, L. and Galli, A. (2004) Development of PCR assay to identify Pseudomonas fluorescens and its biotype. FEMS Microbiol Lett 236, 257-260.
15. Whitton, P.A., Lau, A., Salisbury, A., Whitehouse, J. and Evans, C.S. (2003) Kava lactones and the kava-kava controversy. Photochemistry 64, 673-679.
16. Yamamoto, S., Kasai, H., Arnold, D.L., Jackson, R.W., Viavian, A. and Harayama, S. (2000) Phylogeny of the genus Pseudomonas: intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes. Microbiology 146, 2385-2394.