Efficient one-step chromatographic purification and functional characterization of recombinant human Saposin C

Protein Expression and Purification

Volumn 78, Issue 2, 2011, Pages 209-215

  Motta, Marialetizia ^a   Tatti, Massimo ^a   Martinelli, Simone ^a   Camerini, Serena ^a   Scarpa, Susanna ^b   Crescenzi, Marco ^a   Tartaglia, Marco ^a   Salvioli, Rosa ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Affinity chromatography; Endocytosis; Mass spectrometry; Overexpression; Protein anionic phospholipids interactions; Sphingolipid activator protein

Indexed keywords

RECOMBINANT PROTEIN; SPHINGOLIPID ACTIVATOR PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CHEMISTRY; ENDOCYTOSIS; FIBROBLAST; FLUORESCENCE MICROSCOPY; HUMAN; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

BLOTTING, WESTERN; CELLS, CULTURED; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOCYTOSIS; FIBROBLASTS; HUMANS; MASS SPECTROMETRY; MICROSCOPY, FLUORESCENCE; RECOMBINANT PROTEINS; SAPOSINS;

BACTERIA (MICROORGANISMS);

EID: 79958726494     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.03.015     Document Type: Article

References (25)

1. J. O'Brien, Y. Kishimoto, Saposin proteins: structure, function, and role in human lysosomal storage disorders, FASEB J. 5 (1991) 301-308.
2. Y. Kishimoto, M. Hiraiwa, J.S. O'Brien, Saposins: structure, function, distribution, and molecular genetics, J. Lipid Res. 33 (1992) 1255-1267.
3. W. Furst, K. Sandhoff, Activator proteins and topology of lysosomal sphingolipid catabolism, Biochim. Biophys. Acta 1126 (1992) 1-16.
4. K. Sandhoff, T. Kolter, H. Harzer, Sphingolipid activators proteins, in: C.R. Scriver, A.L. Beaudet, W.S. Sly, D. Valle, B. Childs, K.W. Kinzler, B. Volgstein (Eds.), The Metabolic and Molecular Bases of Inherited Disease, 8th ed., McGraw-Hill, New York, 2001, pp. 3371-3388.
5. A.M. Vaccaro, F. Ciaffoni, M. Tatti, R. Salvioli, A. Barca, D. Tognozzi, C. Scerch, PH-dependent conformational properties of saposins and their interactions with phospholipid membranes, J. Biol. Chem. 270 (1995) 30576-30580.
6. Y. Sun, X. Qi, G.A. Grabowski, Saposin C is required for normal resistance of acid β-glucosidase to proteolytic degradation, J. Biol. Chem. 278 (2003) 31918-31923.
7. H. Christomanou, A. Chabas, T. Pampols, A. Guardiola, Activator protein deficient Gaucher's disease. A second patient with the newly identified lipid storage disorder, Klin. Wochenschr. 67 (1989) 999-1003. (Pubitemid 19239576)
8. D. Schnabel, M. Schroeder, K. Sandhoff, Mutation in the sphingolipid activator protein 2 in a patient with a variant of Gaucher disease, FEBS Lett. 284 (1991) 57-59.
9. A. Tylki-Szymanska, B. Czartoryska, M.T. Vanier, B.J.M.H. Poorthuis, J.A.E. Groener, A. Lugowska, G. Millat, A.M. Vaccaro, E. Jurkiewicz, Non-neuronopathic Gaucher disease due to Saposin C deficiency, Clin. Genet. 72 (2007) 538-542.
10. A.M. Vaccaro, M. Motta, M. Tatti, S. Scarpa, L. Masuelli, M. Bhat, M.T. Vanier, A. Tylki-Szymanska, R. Salvioli, Saposin C mutations in Gaucher disease patients resulting in lysosomal lipid accumulation, Saposin C deficiency, but normal prosaposin processing and sorting, Hum. Mol. Genet. 19 (2010) 2987-2997.
11. A.M. Vaccaro, M. Tatti, F. Ciaffoni, R. Salvioli, B. Maras, A. Barca, Function of Saposin C in the reconstitution of glucosylceramidase by phosphatidylserine liposomes, FEBS Lett. 336 (1993) 159-162. (Pubitemid 23362025)
12. A.M. Vaccaro, M. Tatti, F. Ciaffoni, R. Salvioli, A. Barca, C. Scerch, Effect of Saposins A and C on the enzymatic hydrolysis of liposomal glucosylceramide, J. Biol. Chem. 272 (1997) 16862-16867.
13. A.M. Vaccaro, R. Salvioli, M. Tatti, F. Ciaffoni, Saposins and their interaction with lipids, Neurochem. Res. 24 (1999) 307-314.
14. R. Salvioli, M. Tatti, F. Ciaffoni, A.M. Vaccaro, Further studies on the reconstitution of glucosylceramidase activity by Sap C and anionic phospholipids, FEBS Lett. 472 (2000) 17-21. (Pubitemid 30224578)
15. A.M. Vaccaro, R. Salvioli, A. Barca, M. Tatti, F. Ciaffoni, B. Maras, R. Siciliano, F. Zappacosta, A. Amoresano, P. Pucci, Structural analysis of Saposin C and B, J. Biol. Chem. 270 (1995) 9953-9960.
16. X. Qi, T. Leonova, G. Grabowski, Functional human Saposins expressed in Escherichia coli, J. Biol. Chem. 269 (1994) 16746-16753.
17. E. de Alba, S. Weiler, N. Tjandra, Solution structure of human Saposin C: pH-dependent interaction with phospholipid vesicles, Biochemistry 42 (2003) 14729-14740. (Pubitemid 37553501)
18. V.E. Ahn, P. Leyko, J. Alattia, L. Chien, G.G. Privé, Crystal structures of Saposins A and C, Protein Sci. 15 (2006) 1849-1857. (Pubitemid 44141503)
19. R. Schultz-Heienbrok, N. Remmel, R. Klingenstein, M. Rossocha, K. Sandhoff, W. Saenger, T. Maier, Crystallization and preliminary characterization of three different crystal forms of human Saposin C heterologously expressed in Pichia pastoris, Acta Crystallogr., Sect. F: Struct. Biol. Cryst. Commun. 62 (2006) 117-120.
20. A. Schevchenko, O.N. Jensen, A.V. Podtelejnikov, F. Sagliocco, M. Wilm, O. Vorm, P. Mortensen, A. Schevchenko, H. Boucherie, M. Mann, Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels, Proc. Natl. Acad. Sci. USA 93 (1996) 14440-14445. (Pubitemid 26418985)
21. 370 → Ser) mutation affects the capacity of glucosylceramidase to interact with anionic phospholipid-containing membranes and Saposin C, Biochem. J. 390 (2005) 95-103.
22. D. Amsellem, D. Rodriguez, M.T. Vanier, N. Khayat, G. Millat, M. Campello, C. Guillame, T. Billette De Villemeur, Third case of Gaucher disease with Sap C deficiency and evaluation of twelve months' therapy by miglustat, J. Inherit. Metab. Dis. 28 (Suppl. 1) (2005) 152.
23. A. Sano, N.S. Radin, The carbohydrate moiety of the activator protein for glucosylceramide beta-glucosidase, Biochem. Biophys. Res. Commun. 154 (1988) 1197-1203.
24. A. Sano, T. Hineno, T. Mizuno, K. Kondoh, S. Ueno, Y. Kakimoto, K. Inui, Sphingolipid hydrolase activator proteins and their precursors, Biochem. Biophys. Res. Commun. 165 (1989) 1191-1197. (Pubitemid 20029834)
25. M. Ho, J.S. O' Brien, Gaucher's disease: deficiency of 'acid'-glucosidase and reconstitution of enzyme activity in vitro, Proc. Natl. Acad. Sci. USA 68 (1971) 2810-2813.