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Volumn 415, Issue 2, 2011, Pages 87-96

Integrated microfluidic reverse transcription-polymerase chain reaction for rapid detection of food- or waterborne pathogenic rotavirus

Author keywords

Microfluidics; Online fluorescence detection; Reverse transcription polymerase chain reaction (RT PCR); Rotavirus

Indexed keywords

DIAGNOSIS; FLUIDIC DEVICES; FLUORESCENCE; FOOD MICROBIOLOGY; MICROFLUIDICS; POLYMERS;

EID: 79958289702     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.04.026     Document Type: Article
Times cited : (41)

References (50)
  • 4
    • 33845328836 scopus 로고    scopus 로고
    • The epidemiology and burden of rotavirus in China: A review of the literature from 1983 to 2005
    • DOI 10.1016/j.vaccine.2006.07.054, PII S0264410X06009170
    • E.W. Orenstein, Z.Y. Fang, J. Xu, C.Y. Liu, K.L. Shen, Y. Qian, B.M. Jiang, P.E. Kilgore, R.I. Glass, The epidemiology and burden of rotavirus in China: a review of the literature from 1983 to 2005, Vaccine 25 (2007) 406-413. (Pubitemid 44873147)
    • (2007) Vaccine , vol.25 , Issue.3 , pp. 406-413
    • Orenstein, E.W.1    Fang, Z.Y.2    Xu, J.3    Liu, C.4    Shen, K.5    Qian, Y.6    Jiang, B.7    Kilgore, P.E.8    Glass, R.I.9
  • 5
    • 38549103583 scopus 로고    scopus 로고
    • Rotavirus vaccines: An overview
    • DOI 10.1128/CMR.00029-07
    • P.H. Dennehy Rotavirus vaccines: an overview Clin. Microbiol. Rev. 21 2008 198 208 (Pubitemid 351160015)
    • (2008) Clinical Microbiology Reviews , vol.21 , Issue.1 , pp. 198-208
    • Dennehy, P.H.1
  • 6
    • 26244466537 scopus 로고    scopus 로고
    • Evaluation of rotavirus dsRNA load in specimens and body fluids from experimentally infected juvenile macaques by real-time PCR
    • DOI 10.1016/j.virol.2005.06.048, PII S004268220500406X
    • W. Zhao, M.J. Xia, T.B. Malveo, M. Cantú, M.M. McNeal, A.H. Choi, R.L. Ward, and K. Sestak Evaluation of rotavirus dsRNA load in specimens and body fluids from experimentally infected juvenile macaques by real-time PCR Virology 341 2005 248 256 (Pubitemid 41416734)
    • (2005) Virology , vol.341 , Issue.2 , pp. 248-256
    • Zhao, W.1    Xia, M.2    Bridges-Malveo, T.3    Cantu, M.4    McNeal, M.M.5    Choi, A.H.6    Ward, R.L.7    Sestak, K.8
  • 8
    • 57849101993 scopus 로고    scopus 로고
    • Broadly reactive TaqMan assay for real-time RT-PCR detection of rotavirus in clinical and environmental samples
    • N. Jothikumar, G. Kang, and V.R. Hill Broadly reactive TaqMan assay for real-time RT-PCR detection of rotavirus in clinical and environmental samples J. Virol. Methods 155 2009 126 131
    • (2009) J. Virol. Methods , vol.155 , pp. 126-131
    • Jothikumar, N.1    Kang, G.2    Hill, V.R.3
  • 9
    • 0034713913 scopus 로고    scopus 로고
    • Viruses and bivalve shellfish
    • DOI 10.1016/S0168-1605(00)00248-8, PII S0168160500002488
    • D. Lees Viruses and bivalve shellfish Int. J. Food Microbiol. 59 2000 81 116 (Pubitemid 30408292)
    • (2000) International Journal of Food Microbiology , vol.59 , Issue.1-2 , pp. 81-116
    • Lees, D.1
  • 10
    • 0141615140 scopus 로고    scopus 로고
    • DNA amplification: Does "small" really mean "efficient"?
    • A.J. de Mello DNA amplification: does "small" really mean "efficient"? Lab Chip 1 2001 24 29
    • (2001) Lab Chip , vol.1 , pp. 24-29
    • De Mello, A.J.1
  • 11
    • 33646575914 scopus 로고    scopus 로고
    • Microfluidic single-cell mRNA isolation and analysis
    • DOI 10.1021/ac0519460
    • J.S. Marcus, W.F. Anderson, and S.R. Quake Microfluidic single-cell mRNA isolation and analysis Anal. Chem. 78 2006 3084 3089 (Pubitemid 43726129)
    • (2006) Analytical Chemistry , vol.78 , Issue.9 , pp. 3084-3089
    • Marcus, J.S.1    Anderson, W.F.2    Quake, S.R.3
  • 12
    • 0029989857 scopus 로고    scopus 로고
    • Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device
    • DOI 10.1021/ac960718q
    • A.T. Wooley, D. Hadley, P. Landre, A.J. de Mello, R.A. Mathies, and M.A. Northrup Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device Anal. Chem. 68 1996 4081 4086 (Pubitemid 26419446)
    • (1996) Analytical Chemistry , vol.68 , Issue.23 , pp. 4081-4086
    • Woolley, A.T.1    Hadley, D.2    Landre, P.3    DeMello, A.J.4    Mathies, R.A.5    Northrup, M.A.6
  • 13
    • 0035311755 scopus 로고    scopus 로고
    • Polymerase chain reaction in polymeric microchips: DNA amplification in less than 240 seconds
    • DOI 10.1006/abio.2000.4974
    • B.C. Giordano, J. Ferrance, S. Swedberg, A.F.R. Hühmer, and J.P. Landers Polymerase chain reaction in polymeric microchips: DNA amplification in less than 240 seconds Anal. Biochem. 291 2001 124 132 (Pubitemid 32324533)
    • (2001) Analytical Biochemistry , vol.291 , Issue.1 , pp. 124-132
    • Giordano, B.C.1    Ferrance, J.2    Swedberg, S.3    Huhmer, A.F.R.4    Landers, J.P.5
  • 14
    • 34547839765 scopus 로고    scopus 로고
    • Miniaturized PCR chips for nucleic acid amplification and analysis: Latest advances and future trends
    • DOI 10.1093/nar/gkm389
    • C.S. Zhang, and D. Xing Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends Nucleic Acids Res. 35 2007 4223 4237 (Pubitemid 47244579)
    • (2007) Nucleic Acids Research , vol.35 , Issue.13 , pp. 4223-4237
    • Zhang, C.1    Xing, D.2
  • 16
    • 71249151954 scopus 로고    scopus 로고
    • A microfluidic-based system using reverse transcription polymerase chain reactions for rapid detection of aquaculture diseases
    • K.Y. Lien, S.H. Lee, T.J. Tsai, T.Y. Chen, and G.B. Lee A microfluidic-based system using reverse transcription polymerase chain reactions for rapid detection of aquaculture diseases Microfluid. Nanofluid. 7 2009 795 806
    • (2009) Microfluid. Nanofluid. , vol.7 , pp. 795-806
    • Lien, K.Y.1    Lee, S.H.2    Tsai, T.J.3    Chen, T.Y.4    Lee, G.B.5
  • 17
    • 0034658921 scopus 로고    scopus 로고
    • A miniature integrated device for automated multistep genetic assays
    • R.C. Anderson, X. Su, G.J. Bogdan, and J. Fenton A miniature integrated device for automated multistep genetic assays Nucleic Acids Res. 28 2000 e60
    • (2000) Nucleic Acids Res. , vol.28 , pp. 60
    • Anderson, R.C.1    Su, X.2    Bogdan, G.J.3    Fenton, J.4
  • 18
    • 56549102421 scopus 로고    scopus 로고
    • A polymer lab-on-chip for reverse transcription (RT)-PCR based point-of-care clinical diagnostics
    • S.H. Lee, S.W. Kim, J.Y. Kang, and C.H. Ahn A polymer lab-on-chip for reverse transcription (RT)-PCR based point-of-care clinical diagnostics Lab Chip 8 2008 2121 2127
    • (2008) Lab Chip , vol.8 , pp. 2121-2127
    • Lee, S.H.1    Kim, S.W.2    Kang, J.Y.3    Ahn, C.H.4
  • 19
    • 0036136272 scopus 로고    scopus 로고
    • Improved detection of human enteric viruses in foods by RT-PCR
    • DOI 10.1016/S0166-0934(01)00397-4, PII S0166093401003974
    • A.I. Sair, D.H. D'Souza, C.L. Moe, and L.A. Jaukus Improved detection of human enteric viruses in foods by RT-PCR J. Virol. Methods 100 2002 57 69 (Pubitemid 34003625)
    • (2002) Journal of Virological Methods , vol.100 , Issue.1-2 , pp. 57-69
    • Sair, A.I.1    D'Souza, D.H.2    Moe, C.L.3    Jaykus, L.-A.4
  • 20
    • 33845327545 scopus 로고    scopus 로고
    • Multichannel reverse transcription-polymerase chain reaction microdevice for rapid gene expression and biomarker analysis
    • DOI 10.1021/ac061058k
    • N.M. Toriello, C.N. Liu, and R.A. Mathies Multichannel reverse transcription-polymerase chain reaction microdevice for rapid gene expression and biomarker analysis Anal. Chem. 78 2006 7997 8003 (Pubitemid 44871764)
    • (2006) Analytical Chemistry , vol.78 , Issue.23 , pp. 7997-8003
    • Toriello, N.M.1    Liu, C.N.2    Mathies, R.A.3
  • 21
    • 33748348843 scopus 로고    scopus 로고
    • An integrated microfluidic chip for DNA/RNA amplification, electrophoresis separation, and on-line optical detection
    • F.C. Huang, C.S. Liao, and G.B. Lee An integrated microfluidic chip for DNA/RNA amplification, electrophoresis separation, and on-line optical detection Electrophoresis 27 2006 3297 3305
    • (2006) Electrophoresis , vol.27 , pp. 3297-3305
    • Huang, F.C.1    Liao, C.S.2    Lee, G.B.3
  • 22
    • 34548169367 scopus 로고    scopus 로고
    • Microfluidic chips for detecting the t(4;14) translocation and monitoring disease during treatment using reverse transcriptase-polymerase chain reaction analysis of IgH-MMSET hybrid transcripts
    • DOI 10.2353/jmoldx.2007.060149
    • J. VanDijken, G.V. Kaigala, J. Lauzon, A. Atrazhev, S. Adamia, B.J. Taylor, T. Reiman, A.R. Belch, C.J. Backhouse, and L.M. Pilarski Microfluidic chips for detecting the t(4;14) translocation and monitoring disease during treatment using reverse transcriptase-polymerase chain reaction analysis of IgH-MMSET hybrid transcripts J. Mol. Diagn. 9 2007 358 367 (Pubitemid 47307234)
    • (2007) Journal of Molecular Diagnostics , vol.9 , Issue.3 , pp. 358-367
    • VanDijken, J.1    Kaigala, G.V.2    Lauzon, J.3    Atrazhev, A.4    Adamia, S.5    Taylor, B.J.6    Reiman, T.7    Belch, A.R.8    Backhouse, C.J.9    Pilarski, L.M.10
  • 23
    • 40049097270 scopus 로고    scopus 로고
    • An inexpensive and portable microchip-based platform for integrated RT-PCR and capillary electrophoresis
    • DOI 10.1039/b714308g
    • G.V. Kaigala, V.N. Hoang, A. Stickel, J. Lauzon, D. Manage, L.M. Pilarski, and C.J. Backhouse An inexpensive and portable microchip-based platform for integrated RT-PCR and capillary electrophoresis Analyst 133 2008 331 338 (Pubitemid 351322606)
    • (2008) Analyst , vol.133 , Issue.3 , pp. 331-338
    • Kaigala, G.V.1    Hoang, V.N.2    Stickel, A.3    Lauzon, J.4    Manage, D.5    Pilarski, L.M.6    Backhouse, C.J.7
  • 24
    • 33846587591 scopus 로고    scopus 로고
    • Integrated reverse transcription polymerase chain reaction systems for virus detection
    • K.Y. Lien, W.C. Lee, H.Y. Lei, and G.B. Lee Integrated reverse transcription polymerase chain reaction systems for virus detection Biosens. Bioelectron. 22 2007 1739 1748
    • (2007) Biosens. Bioelectron. , vol.22 , pp. 1739-1748
    • Lien, K.Y.1    Lee, W.C.2    Lei, H.Y.3    Lee, G.B.4
  • 25
    • 36849027206 scopus 로고    scopus 로고
    • An integrated microfluidic system using magnetic beads for virus detection
    • W.C. Lee, L.Y. Lien, G.B. Lee, and H.Y. Lei An integrated microfluidic system using magnetic beads for virus detection Diagn. Microbiol. Infect. Dis. 60 2008 51 58
    • (2008) Diagn. Microbiol. Infect. Dis. , vol.60 , pp. 51-58
    • Lee, W.C.1    Lien, L.Y.2    Lee, G.B.3    Lei, H.Y.4
  • 28
    • 67349098528 scopus 로고    scopus 로고
    • A two-dimensional, self-compensated, microthermal cycler for one-step reverse transcription polymerase chain reaction applications
    • T.M. Hsieh, C.H. Luo, J.H. Wang, J.L. Lin, K.Y. Lien, and G.B. Lee A two-dimensional, self-compensated, microthermal cycler for one-step reverse transcription polymerase chain reaction applications Microfluid. Nanofluid. 6 2009 797 809
    • (2009) Microfluid. Nanofluid. , vol.6 , pp. 797-809
    • Hsieh, T.M.1    Luo, C.H.2    Wang, J.H.3    Lin, J.L.4    Lien, K.Y.5    Lee, G.B.6
  • 29
    • 32444435330 scopus 로고    scopus 로고
    • Parallel picoliter RT-PCR assays using microfluidics
    • DOI 10.1021/ac0513865
    • J.S. Marcus, W.F. Anderson, and S.R. Quake Parallel picoliter RT-PCR assays using microfluidics Anal. Chem. 78 2006 956 958 (Pubitemid 43228605)
    • (2006) Analytical Chemistry , vol.78 , Issue.3 , pp. 956-958
    • Marcus, J.S.1    Anderson, W.F.2    Quake, S.R.3
  • 30
    • 74049083827 scopus 로고    scopus 로고
    • Droplet-based gene expression analysis using a device with magnetic force-based droplet-handling system
    • M. Okochi, H. Tsuchiya, F. Kumazawa, M. Shikida, and H. Honda Droplet-based gene expression analysis using a device with magnetic force-based droplet-handling system J. Biosci. Bioeng. 109 2010 193 197
    • (2010) J. Biosci. Bioeng. , vol.109 , pp. 193-197
    • Okochi, M.1    Tsuchiya, H.2    Kumazawa, F.3    Shikida, M.4    Honda, H.5
  • 32
    • 79952264969 scopus 로고    scopus 로고
    • An integrated, valveless system for microfluidic purification and reverse transcription-PCR amplification of RNA for detection of infectious agents
    • K.A. Hagan, C.R. Reedy, M.L. Uchimoto, D. Basu, D.A. Engel, and J.P. Landers An integrated, valveless system for microfluidic purification and reverse transcription-PCR amplification of RNA for detection of infectious agents Lab Chip 11 2011 957 961
    • (2011) Lab Chip , vol.11 , pp. 957-961
    • Hagan, K.A.1    Reedy, C.R.2    Uchimoto, M.L.3    Basu, D.4    Engel, D.A.5    Landers, J.P.6
  • 33
    • 0037439263 scopus 로고    scopus 로고
    • Microfabricated device for DNA and RNA amplification by continuous-flow polymerase chain reaction and reverse transcription-polymerase chain reaction with cycle number selection
    • DOI 10.1021/ac0260239
    • P.J. Obeid, T.K. Christopoulos, H.J. Crabtree, and C.J. Backhouse Microfabricated device for DNA and RNA amplification by continuous-flow polymerase chain reaction and reverse transcription-polymerase chain reaction with cycle number selection Anal. Chem. 75 2003 288 295 (Pubitemid 36119775)
    • (2003) Analytical Chemistry , vol.75 , Issue.2 , pp. 288-295
    • Obeid, P.J.1    Christopoulos, T.K.2    Crabtree, H.J.3    Backhouse, C.J.4
  • 34
    • 0141426476 scopus 로고    scopus 로고
    • Continuous-flow DNA and RNA amplification chip combined with laser-induced fluorescence detection
    • DOI 10.1016/S0003-2670(03)00898-5
    • P.J. Obeid, and T.K. Christopoulos Continuous-flow DNA and RNA amplification chip combined with laser-induced fluorescence detection Anal. Chim. Acta 494 2003 1 9 (Pubitemid 37186811)
    • (2003) Analytica Chimica Acta , vol.494 , Issue.1-2 , pp. 1-9
    • Obeid, P.J.1    Christopoulos, T.K.2
  • 35
    • 33747343721 scopus 로고    scopus 로고
    • SU-8 based continuous-flow RT-PCR bio-chips under high-precision temperature control
    • N.C. Tsai, and C.Y. Sue SU-8 based continuous-flow RT-PCR bio-chips under high-precision temperature control Biosens. Bioelectron. 22 2006 313 317
    • (2006) Biosens. Bioelectron. , vol.22 , pp. 313-317
    • Tsai, N.C.1    Sue, C.Y.2
  • 36
    • 36048995958 scopus 로고    scopus 로고
    • Reverse transcription-polymerase chain reaction (RT-PCR) in flow-through micro-reactors: Thermal and fluidic concepts
    • J. Felbel, A. Reichert, M. Kielpinski, M. Urban, T. Henkel, N. Häfner, M. Dürst, and J. Weber Reverse transcription-polymerase chain reaction (RT-PCR) in flow-through micro-reactors: thermal and fluidic concepts Chem. Eng. J. 135 2008 S298 S302
    • (2008) Chem. Eng. J. , vol.135
    • Felbel, J.1    Reichert, A.2    Kielpinski, M.3    Urban, M.4    Henkel, T.5    Häfner, N.6    Dürst, M.7    Weber, J.8
  • 39
    • 60849111937 scopus 로고    scopus 로고
    • Parallel DNA amplification by convective polymerase chain reaction with various annealing temperatures on a thermal gradient device
    • C.S. Zhang, and D. Xing Parallel DNA amplification by convective polymerase chain reaction with various annealing temperatures on a thermal gradient device Anal. Biochem. 387 2009 102 112
    • (2009) Anal. Biochem. , vol.387 , pp. 102-112
    • Zhang, C.S.1    Xing, D.2
  • 40
    • 57849133899 scopus 로고    scopus 로고
    • Rapid detection of genetically modified organisms on a continuous-flow polymerase chain reaction microfluidics
    • Y.Y. Li, D. Xing, and C.S. Zhang Rapid detection of genetically modified organisms on a continuous-flow polymerase chain reaction microfluidics Anal. Biochem. 385 2009 42 49
    • (2009) Anal. Biochem. , vol.385 , pp. 42-49
    • Li, Y.Y.1    Xing, D.2    Zhang, C.S.3
  • 41
    • 38649123389 scopus 로고    scopus 로고
    • Simultaneous detection of norovirus and rotavirus in oysters by multiplex RT-PCR
    • X.X. Kou, Q.P. Wu, D.P. Wang, and J.M. Zhang Simultaneous detection of norovirus and rotavirus in oysters by multiplex RT-PCR Food Control 19 2008 722 726
    • (2008) Food Control , vol.19 , pp. 722-726
    • Kou, X.X.1    Wu, Q.P.2    Wang, D.P.3    Zhang, J.M.4
  • 42
    • 0036079139 scopus 로고    scopus 로고
    • A nanoliter rotary device for polymerase chain reaction
    • DOI 10.1002/1522-2683(20020 5)23:10<1531::AID-ELPS1531>3.0.CO;2-D
    • J. Liu, M. Enzelberger, and S. Quake A nanoliter rotary device for polymerase chain reaction Electrophoresis 23 2002 1531 1536 (Pubitemid 34649584)
    • (2002) Electrophoresis , vol.23 , Issue.10 , pp. 1531-1536
    • Liu, J.1    Enzelberger, M.2    Quake, S.3
  • 43
    • 34250163888 scopus 로고    scopus 로고
    • Continuous-flow PCR microfluidics for rapid DNA amplification using thin film heater with low thermal mass
    • DOI 10.1080/00032710701298446, PII 779430871
    • C.S. Zhang, D. Xing, and J.L. Xu Continuous-flow PCR microfluidics for rapid DNA amplification using thin film heater with low thermal mass Anal. Lett. 40 2007 1672 1685 (Pubitemid 46897556)
    • (2007) Analytical Letters , vol.40 , Issue.9 , pp. 1672-1685
    • Zhang, C.1    Xing, D.2    Xu, J.3
  • 44
    • 0242322735 scopus 로고    scopus 로고
    • Cylindrical Compact Thermal-Cycling Device for Continuous-Flow Polymerase Chain Reaction
    • DOI 10.1021/ac0346959
    • N. Park, S. Kim, and J.H. Hahn Cylindrical compact thermal-cycling device for continuous-flow polymerase chain reaction Anal. Chem. 75 2003 6029 6033 (Pubitemid 37356488)
    • (2003) Analytical Chemistry , vol.75 , Issue.21 , pp. 6029-6033
    • Park, N.1    Kim, S.2    Hahn, J.H.3
  • 45
    • 0037233259 scopus 로고    scopus 로고
    • Continuous segmented-flow polymerase chain reaction for high-throughput miniaturized DNA amplification
    • DOI 10.1021/ac0204146
    • M. Curcio, and J. Roeraade Continuous segmented-flow polymerase chain reaction for high-throughput miniaturized DNA amplification Anal. Chem. 75 2003 1 7 (Pubitemid 36090071)
    • (2003) Analytical Chemistry , vol.75 , Issue.1 , pp. 1-7
    • Curcio, M.1    Roeraade, J.2
  • 46
    • 20444390163 scopus 로고    scopus 로고
    • Contamination-free continuous flow microfluidic polymerase chain reaction for quantitative and clinical applications
    • DOI 10.1021/ac050031i
    • K.D. Dorfman, M. Chabert, J.H. Codarbox, G. Rousseau, P. de Cremoux, and J.L. Viovy Contamination-free continuous flow microfluidic polymerase chain reaction for quantitative and clinical applications Anal. Chem. 77 2005 3700 3704 (Pubitemid 40799864)
    • (2005) Analytical Chemistry , vol.77 , Issue.11 , pp. 3700-3704
    • Dorfman, K.D.1    Chabert, M.2    Codarbox, J.-H.3    Rousseau, G.4    De Cremoux, P.5    Viovy, J.-L.6
  • 47
    • 33751212797 scopus 로고    scopus 로고
    • Automated microdroplet platform for sample manipulation and polymerase chain reaction
    • DOI 10.1021/ac061205e
    • M. Chabert, K.D. Dorfman, P. de Cremoux, J. Roeraade, and J.L. Viovy Automated microdroplet platform for sample manipulation and polymerase chain reaction Anal. Chem. 78 2006 7722 7728 (Pubitemid 44788715)
    • (2006) Analytical Chemistry , vol.78 , Issue.22 , pp. 7722-7728
    • Chabert, M.1    Dorfman, K.D.2    De Cremoux, P.3    Roeraade, J.4    Viovy, J.-L.5
  • 48
    • 77649232031 scopus 로고    scopus 로고
    • High-throughput droplet PCR
    • A.L. Markey, S. Mohr, and P.J.R. Day High-throughput droplet PCR Methods 50 2010 277 281
    • (2010) Methods , vol.50 , pp. 277-281
    • Markey, A.L.1    Mohr, S.2    Day, P.J.R.3
  • 49
    • 34548303859 scopus 로고    scopus 로고
    • Gene transcript amplification from cell lysates in continuous-flow microfluidic devices
    • DOI 10.1007/s10544-007-9083-1
    • A. Gonzalez, D. Ciobanu, M. Sayers, N. Sirr, T. Dalton, and M. Davies Gene transcript amplification from cell lysates in continuous-flow microfluidic devices Biomed. Microdevices 9 2007 729 736 (Pubitemid 47337896)
    • (2007) Biomedical Microdevices , vol.9 , Issue.5 , pp. 729-736
    • Gonzalez, A.1    Ciobanu, D.2    Sayers, M.3    Sirr, N.4    Dalton, T.5    Davies, M.6
  • 50
    • 79958126754 scopus 로고    scopus 로고
    • Long target droplet polymerase chain reaction with a microfluidic device for high-throughput detection of pathogenic bacteria at clinical sensitivity
    • J.R. Peham, W. Grienauer, H. Steiner, R. Heer, M.J. Vellekoop, C. Nöhammer, and H. Wiesinger-Mayr Long target droplet polymerase chain reaction with a microfluidic device for high-throughput detection of pathogenic bacteria at clinical sensitivity Biomed. Microdevices 13 2011 463 473
    • (2011) Biomed. Microdevices , vol.13 , pp. 463-473
    • Peham, J.R.1    Grienauer, W.2    Steiner, H.3    Heer, R.4    Vellekoop, M.J.5    Nöhammer, C.6    Wiesinger-Mayr, H.7


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