Automated Analysis of FISH-Stained HER2/neu Samples with Metafer

Methods in Molecular Biology

Volumn 724, Issue , 2011, Pages 91-103

  Schunck, Christian ^a   Mohammad, Eiman ^b  

^a MetaSystems   (Germany)

^b KUWAIT UNIVERSITY   (Kuwait)

Author keywords

Breast cancer; Digital microscopy; HER2 neu; Metafer PV; PathVysion ; Slide scanning; Tile sampling

Indexed keywords

DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; EPIDERMAL GROWTH FACTOR RECEPTOR 2; FORMALDEHYDE; PARAFFIN;

ARTICLE; AUTOMATION; DNA DENATURATION; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; INSTRUMENTATION; METABOLISM; METHODOLOGY; STAINING; TISSUE FIXATION;

AUTOMATION; FORMALDEHYDE; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; INDICATORS AND REAGENTS; NUCLEIC ACID DENATURATION; PARAFFIN EMBEDDING; RECEPTOR, ERBB-2; STAINING AND LABELING; TISSUE FIXATION;

EID: 79958288907     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-055-3_6     Document Type: Chapter

References (4)

1. Stevens, R., Almanaseer, I., Gonzalez, M., Caglar, D., Knudson, R.A., Ketterling, R.P., Schrock, D.S., Seemayer, T.A., and Bridge, J.A. (2007) Analysis of HER2 gene amplification using an automated fluorescence in situ hybridization signal enumeration system. Journal of Molecular Diagnostics 9, 144–50.
2. Dendukuri, N., Khetani, K., McIsaac, M., and Brophy, J. (2007) Testing for HER2-positive breast cancer: a systematic review and cost-effectiveness analysis. CMAJ 176, 1429–34.
3. Lörch, T., Piper, J., and Tomisek, J.D. (2002) “Tile Sampling”: a new method for the auto- mated quantitative analysis of samples with high cell density and its application to HER2 scanning. In: Proceedings of the Third Euroconference on Quantitative Molecular Cytogenetics. Rosenön, Stockholm, Sweden.
4. Piper, J., Lörch, T., Poole, I., and Tomisek, J.D. (2002) Computing the HER2:CEP-17 ratio of tumour cells in breast cancer tissue sections by analysois of the FISH spot counts of a tiles sampling. In: Proceedings of the Third Euroconference on Quantitative Molecular Cytogenetics. Rosenön, Stockholm, Sweden.