Utility of a direct dual-mode development analysis on blotted protein mixtures

Analytical Biochemistry

Volumn 415, Issue 2, 2011, Pages 116-125

  Rae, Tracey ^a   Bonn, Ryan ^a   Lang, Ewa ^a   Stamenova, Svetoslava ^a   Burgos, Glamarie ^a   Rupprecht, Kevin ^a   Fishpaugh, Jeffrey ^a  

^a ABBOTT DIAGNOSTICS   (United States)

Author keywords

Antigen identification; Electroblot; Immunoblot; Lectin blot; Multimode detection

Indexed keywords

CHEMICAL DETECTION; ELECTROPHORESIS; MIXTURES;

ELECTROBLOT; IMMUNOBLOTS; LECTIN BLOT; MULTIMODES; PROTEIN IDENTIFICATION; PROTEIN RECOGNITION; QUANTITATIVE INFORMATION; TWO DIMENSIONAL ELECTROPHORESIS;

PROTEINS;

FLUORESCENT DYE; HEMOPEXIN; LECTIN; NEUTROPHIL GELATINASE ASSOCIATED LIPOCALIN; RECOMBINANT PROTEIN;

ANTIBODY DETECTION; ARTICLE; CONTROLLED STUDY; HUMAN; IMAGE ANALYSIS; IMMUNOBLOTTING; PRIORITY JOURNAL; PROTEIN ANALYSIS; QUANTITATIVE ANALYSIS; STAINING; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 79958267108     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.04.021     Document Type: Article

References (18)

1. B. Magi, L. Bini, B. Marzocchi, S. Liberatori, R. Raggiaschi, and V. Pallini Immunoaffinity identification of 2-DE separated proteins Methods Mol. Biol. 112 1999 431 443
2. C.A. McManus, P.M. Donoghue, and M.J. Dunn A fluorescent codetection system for immunoblotting and proteomics through ECL-Plex and CyDye labeling Methods Mol. Biol. 536 2009 515 526
3. W. Steffen, and R.W. Linck Multiple immunoblot: a sensitive technique to stain proteins and detect multiple antigens on a single two-dimensional replica Electrophoresis 10 1989 714 718 (Pubitemid 19258136)
4. P.T. Duong, and F.N. Chang A simple method for assigning multiple immunogens to their protein on a two-dimensional blot and its application to asthma-causing allergens Electrophoresis 22 2001 2098 2102 (Pubitemid 32621940)
5. N. Shojaee, W.F. Patton, M.J. Lim, and D. Shepro Pyrogallol red-molybdate: a reversible, metal chelate stain for detection of proteins immobilized on membrane supports Electrophoresis 17 1996 687 693 (Pubitemid 26158530)
6. J.C. Sanchez, P. Wirth, S. Jaccoud, R.D. Appel, C. Sarto, M.R. Wilkins, and D.F. Hochstrasser Simultaneous analysis of cyclin and oncogene expression using multiple monoclonal antibody immunoblots Electrophoresis 18 1997 638 641 (Pubitemid 27174459)
7. C. Vettermann, H.M. Jack, and D. Mielenz A colloidal silver staining-destaining method for precise assignment of immunoreactive spots in two-dimensional protein patterns Anal. Biochem. 308 2002 381 387 (Pubitemid 35177153)
8. H. Kawasaki, A. Okayama, Y. Iwafune, S. Yahagi, N. Arakawa, and H. Hirano Multiplex detection and identification of proteins on a PVDF membrane blocked with a synthetic polymer-based reagent Electrophoresis 29 2008 4377 4380
9. A. Ducret, C. Desponts, S. Desmarais, M.J. Gresser, and C. Ramachandran A general method for the rapid characterization of tyrosine-phosphorylated proteins by mini two-dimensional gel electrophoresis Electrophoresis 21 2000 2196 2208 (Pubitemid 30435819)
10. P.M. Donoghue, C.A. McManus, N.M. O'Donoghue, S.R. Pennington, and M.J. Dunn CyDye immunoblotting for proteomics: co-detection of specific immunoreactive and total protein profiles Proteomics 6 2006 6400 6404 (Pubitemid 46048379)
11. F.J. Alba, S. Bartolome, A. Bermudez, and J.R. Daban Fluorescent labeling of proteins and its application to SDS-PAGE and Western blotting Methods Mol. Biol. 536 2009 407 416
12. M.J. Dunn Detection of total proteins on Western blots of 2-D polyacrylamide gels Methods Mol. Biol. 112 1999 319 329
13. I. Miller, J. Crawford, and E. Gianazza Protein stains for proteomic applications: which, when, why? Proteomics 6 2006 5385 5408 (Pubitemid 44683210)
14. R.M. Leimgruber, J.P. Malone, M.R. Radabaugh, M.L. LaPorte, B.N. Violand, and J.B. Monahan Development of improved cell lysis, solubilization, and imaging approaches for proteomic analyses Proteomics 2 2002 135 144 (Pubitemid 34273082)
15. W.F. Patton A thousand points of light: the application of fluorescence detection technologies to two-dimensional gel electrophoresis and proteomics Electrophoresis 21 2000 1123 1144 (Pubitemid 30230778)
16. K. Berggren, T.H. Steinberg, W.M. Lauber, J.A. Carroll, M.F. Lopez, E. Chernokalskaya, L. Zieske, Z. Diwu, R.P. Haugland, and W.F. Patton A luminescent ruthenium complex for ultrasensitive detection of proteins immobilized on membrane supports Anal. Biochem. 276 1999 129 143 (Pubitemid 30013033)
17. C. Kemper, K. Berggren, Z. Diwu, and W.F. Patton An improved, luminescent europium-based stain for detection of electroblotted proteins on nitrocellulose or polyvinylidene difluoride membranes Electrophoresis 22 2001 881 889 (Pubitemid 32318410)
18. A. Gorg, W. Weiss, and M.J. Dunn Current two-dimensional electrophoresis technology for proteomics Proteomics 4 2004 3665 3685 (Pubitemid 39657447)