Comparison of three detergent-free protein extraction protocols for white adipose tissue

Analytical Biochemistry

Volumn 415, Issue 2, 2011, Pages 215-217

  Sajic, Tatjana ^a   Hopfgartner, Gérard ^a   Szanto, Ildiko ^a   Varesio, Emmanuel ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ELECTRODEPOSITION; ELECTROSPRAY IONIZATION; ETHANOL; EXTRACTION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOLECULAR BIOLOGY; POSITIVE IONS; PROTEINS; TISSUE;

CELLULAR COMPARTMENTS; CENTRIFUGAL FRACTIONATION; COMPARATIVE STUDIES; LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION-MASS SPECTROMETRIES; PROTEIN EXTRACTION; PROTEIN RECOVERY; TRIFLUOROETHANOL; WHITE ADIPOSE TISSUES;

TISSUE ENGINEERING;

BUFFER; DETERGENT; TRIFLUOROETHANOL;

ANIMAL TISSUE; ARTICLE; BLIGH AND DYER METHOD; CENTRIFUGATION; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; INTERMETHOD COMPARISON; INTRACELLULAR SPACE; LIQUID CHROMATOGRAPHY; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; WHITE ADIPOSE TISSUE;

EID: 79958265644     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.04.023     Document Type: Article

References (16)

1. S. Galic, J.S. Oakhill, and G.R. Steinberg Adipose tissue as an endocrine organ Mol. Cell. Endocrinol. 316 2010 129 139
2. X. Xie, Z. Yi, B. Bowen, C. Wolf, C.R. Flynn, S. Sinha, L.J. Mandarino, and C. Meyer Characterization of the human adipocyte proteome and reproducibility of protein abundance by one-dimensional gel electrophoresis and HPLC-ESI-MS/MS J. Proteome Res. 9 2010 4521 4534
3. D.J. Murphy The biogenesis functions of lipid bodies in animals plants and microorganisms Prog. Lipid Res. 40 2001 325 438 (Pubitemid 32727212)
4. S. Boulant, R. Montserret, R.G. Hope, M. Ratinier, P. Targett-Adams, J.P. Lavergne, F. Penin, and J. McLauchlan Structural determinants that target the hepatitis C virus core protein to lipid droplets J. Biol. Chem. 281 2006 22236 22247 (Pubitemid 44181927)
5. V. Subramanian, A. Garcia, A. Sekowski, and D.L. Brasaemle Hydrophobic sequences target and anchor perilipin A to lipid droplets J. Lipid Res. 45 2004 1983 1991 (Pubitemid 40990892)
6. D. Lichtenberg, E. Opatowski, and M.M. Kozlov Phase boundaries in mixtures of membrane-forming amphiphiles and micelle-forming amphiphiles Biochim. Biophys. Acta 1508 2000 1 19
7. A.M. Seddon, P. Curnow, and P.J. Booth Membrane proteins lipids and detergents: not just a soap opera Biochim. Biophys. Acta 1666 2004 105 117 (Pubitemid 39425201)
8. Y.G. Yeung, E. Nieves, R.H. Angeletti, and E.R. Stanley Removal of detergents from protein digests for mass spectrometry analysis Anal. Biochem. 382 2008 135 137
9. P.S. Gullicksen, R.G. Dean, and C.A. Baile Detection of DNA fragmentation apoptotic proteins and quantification of uncoupling protein expression by real-time RT-PCR in adipose tissue J. Biochem. Biophys. Methods 58 2004 1 13 (Pubitemid 37352726)
10. T. Vaisar Proteomic analysis of lipid-protein complexes J. Lipid Res. 50 2009 781 786
11. E.G. Bligh, and W.J. Dyer A rapid method of total lipid extraction and purification Can. J. Biochem. Physiol. 37 1959 911 917
12. B. Akitake, R.E. Spelbrink, A. Anishkin, J.A. Killian, B. de Kruijff, and S. Sukharev 2,2,2-Trifluoroethanol changes the transition kinetics and subunit interactions in the small bacterial mechanosensitive channel MscS Biophys. J. 92 2007 2771 2784 (Pubitemid 46557852)
13. L.G. Alexander, R.B. Sessions, A.R. Clarke, A.S. Tatham, P.R. Shewry, and J.A. Napier Characterization and modelling of the hydrophobic domain of a sunflower oleosin Planta 214 2002 546 551 (Pubitemid 40831769)
14. J.M. Deshusses, J.A. Burgess, A. Scherl, Y. Wenger, N. Walter, V. Converset, S. Paesano, G.L. Corthals, D.F. Hochstrasser, and J.C. Sanchez Exploitation of specific properties of trifluoroethanol for extraction and separation of membrane proteins Proteomics 3 2003 1418 1424 (Pubitemid 37048066)
15. A. De Pauw, S. Tejerina, M. Raes, J. Keijer, and T. Arnould Mitochondrial (dys)function in adipocyte (de)differentiation and systemic metabolic alterations Am. J. Pathol. 175 2009 927 939
16. K. Li, Y. Li, J.M. Shelton, J.A. Richardson, E. Spencer, Z.J. Chen, X. Wang, and R.S. Williams Cytochrome c deficiency causes embryonic lethality and attenuates stress-induced apoptosis Cell 101 2000 389 399