Modified phosphatidylserine-dependent antithrombin ELISA enables identification of patients negative for other antiphospholipid antibodies and also detects low avidity antibodies

Clinical Chemistry and Laboratory Medicine

Volumn 49, Issue 6, 2011, Pages 1011-1018

  Žigon, Polona ^a   Ambrožič, Aleš ^a   Čučnik, Saša ^a   Kveder, Tanja ^a   Rozman, Blaž ^a   Božič, Borut ^a,b  

^a UNIVERSITY MEDICAL CENTRE LJUBLJANA   (Slovenia)

^b UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

antiphospholipid antibody; antiphospholipid syndrome; antiprothrombin antibodies; enzyme immunoassay; phosphatidylserine dependent antiprothrombin antibodies

Indexed keywords

PHOSPHATIDYLSERINE; PHOSPHOLIPID ANTIBODY; PROTHROMBIN ANTIBODY;

ADOLESCENT; ADULT; AGED; ANTIBODY DETECTION; ANTIGEN ANTIBODY COMPLEX; ANTIGEN BINDING; ANTIPHOSPHOLIPID SYNDROME; ARTICLE; BLOOD DONOR; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; RISK FACTOR; SENSITIVITY AND SPECIFICITY; SURFACE PROPERTY;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANIMALS; ANTIBODIES, ANTIPHOSPHOLIPID; ANTIBODY AFFINITY; ANTITHROMBINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; MALE; MIDDLE AGED; PHOSPHATIDYLSERINES; PROTHROMBIN; YOUNG ADULT;

PHOS;

EID: 79958263734     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/CCLM.2011.162     Document Type: Article

References (35)

1. Wilson WA, Gharavi AE, Koike T, Lockshin MD, Branch DW, Piette JC, et al. International consensus statement on preliminary classification criteria for definite antiphospholipid syndrome: report of an international workshop. Arthritis Rheum 1999;42: 1309-11. (Pubitemid 30265586)
2. Miyakis S, Lockshin MD, Atsumi T, Branch DW, Brey RL, Cervera R, et al. International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS). J Thromb Haemost 2006;4:295-306.
3. Atsumi T, Ieko M, Bertolaccini ML, Ichikawa K, Tsutsumi A, Matsuura E, et al. Association of autoantibodies against the phos-phatidylserine-prothrombin complex with manifestations of the antiphospholipid syndrome and with the presence of lupus anticoagulant. Arthritis Rheum 2000;43:1982-93.
4. Bertolaccini ML, Atsumi T, Koike T, Hughes GR, Khamashta MA. Antiprothrombin antibodies detected in two different assay systems. Prevalence and clinical significance in systemic lupus erythematosus. Thromb Haemost 2005;93:289-97. (Pubitemid 40253734)
5. Nojima J, Iwatani Y, Suehisa E, Kuratsune H, Kanakura Y. The presence of anti-phosphatidylserine/prothrombin antibodies as risk factor for both arterial and venous thrombosis in patients with systemic lupus erythematosus. Haematologica 2006;91: 699-702. (Pubitemid 43799474)
6. Arvieux J, Darnige L, Caron C, Reber G, Bensa JC, Colomb MG. Development of an ELISA for autoantibodies to prothrom-bin showing their prevalence in patients with lupus anticoagulants. Thromb Haemost 1995;74:1120-5.
7. Matsuda J, Saitoh N, Gotoh M, Kawasugi K, Gohchi K, Tsukamoto M. Phosphatidyl serine-dependent antiprothrombin antibody is exclusive to patients with lupus anticoagulant. Br J Rheumatol 1996;35:589-91.
8. Galli M, Beretta G, Daldossi M, Bevers EM, Barbui T. Different anticoagulant and immunological properties of anti-pro-thrombin antibodies in patients with antiphospholipid antibodies. Thromb Haemost 1997;77:486-91. (Pubitemid 27121014)
9. Matsuda J, Sanaka T, Nishizawa A, Gotoh M, Gohchi K. Two antiprothrombin antibodies against prothrombin and prothrom-bin-phosphatidyl serine show partial but not total identity. Blood Coagul Fibrinolysis 2002;13:697-702. (Pubitemid 36026826)
10. Cucnik S, Kveder T, Bozic B. Heterogeneity of anti-prothrom-bin antibodies, detected by different ELISAs. Lupus 2002;11; 578.
11. Galli M, Barbui T. Antiprothrombin antibodies: detection and clinical significance in the antiphospholipid syndrome. Blood 1999;93:2149-57. (Pubitemid 29153614)
12. Matsuura E, Igarashi Y, Yasuda T, Triplett DA, Koike T. Anti-cardiolipin antibodies recognize beta 2-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface. J Exp Med 1994;179:457-62. (Pubitemid 24058733)
13. Reddel SW, Wang YX, Krilis SA. Anti-beta2-glycoprotein I autoantibodies require an antigen density threshold, consistent with divalent binding. Lupus 2003;12:37-45. (Pubitemid 36172661)
14. Nelsestuen GL. Interactions of vitamin K-dependent proteins with calcium ions and phospholipid membranes. Fed Proc 1978;37:2621-5. (Pubitemid 9055193)
15. Wu JR, Lentz BR. Phospholipid-specific conformational changes in human prothrombin upon binding to procoagulant acidic lipid membranes. Thromb Haemost 1994;71:596-604. (Pubitemid 24132958)
16. Rao LV, Hoang AD, Rapaport SI. Mechanism and effects of the binding of lupus anticoagulant IgG and prothrombin to surface phospholipid. Blood 1996;88:4173-82. (Pubitemid 26399163)
17. Cucnik S, Kveder T, Krizaj I, Rozman B, Bozic B. High avidity anti-beta 2-glycoprotein I antibodies in patients with antiphos-pholipid syndrome. Ann Rheum Dis 2004;63:1478-82. (Pubitemid 39433954)
18. Forastiero RR, Martinuzzo ME, Cerrato GS, Kordich LC, Carreras LO. Relationship of anti beta2-glycoprotein I and anti prothrombin antibodies to thrombosis and pregnancy loss in patients with antiphospholipid antibodies. Thromb Haemost 1997;78:1008-14. (Pubitemid 27417292)
19. Bozic B, Kveder T, Stegnar M, Morosini-Berus E, Kos-Golja M, Peternel P, et al. Influence of degraded phosphatidylserine on binding of antiphospholipid antibodies. Int Arch Allergy Immunol 1997; 112: 19-26. (Pubitemid 26418377)
20. Loizou S, McCrea JD, Rudge JC, Reynolds R, Boyle CC, Harris EN. Measurement of anticardiolipin antibodies by an enzyme-linked immunosorbent assay (ELISA). Standardization and quantitation of results. Clin Exp Rheumatol 1985;62:738-45. (Pubitemid 16250493)
21. Cucnik S, Ambrozic A, Bozic B, Skitek M, Kveder T. Anti-beta2- glycoprotein I ELISA: methodology, determination of cut-off values in 434 healthy Caucasians and evaluation of monoclonal antibodies as possible international standards. Clin Chem Lab Med 2000;38:777-83.
22. Reber G, Schousboe I, Tincani A, Sanmarco M, Kveder T, De MP, et al. Inter-laboratory variability of anti-beta2-glycoprotein I measurement. A collaborative study in the frame of the European Forum on Antiphospholipid Antibodies Standardization Group. Thromb Haemost 2002;88:66-73. (Pubitemid 34752269)
23. Brandt JT, Triplett DA, Alving B, Scharrer I. Criteria for the diagnosis of lupus anticoagulants: an update. On behalf of the Subcommittee on Lupus Anticoagulant/Antiphospholipid Antibody of the Scientific and Standardisation Committee of the ISTH. Thromb Haemost 1995;74:1185-90.
24. Exner T, Papadopoulos G, Koutts J. Use of a simplified dilute Russell's viper venom time (DRVVT) confirms heterogeneity among 'lupus anticoagulants'. Blood Coagul Fibrinolysis 1990; 1:259-66.
25. Bertolaccini ML. Antiprothrombin Antibodies. In: Khamashta MA, editor. Hughes Syndrome. 2nd ed. Springer, 2006:319-28.
26. Amengual O, Atsumi T, Koike T. Specificities, properties, and clinical significance of antiprothrombin antibodies. Arthritis Rheum 2003;48:886-95. (Pubitemid 36418230)
27. Atsumi T, Koike T. Antiprothrombin antibody: why do we need more assays? Lupus 2010;19:436-9.
28. Bardin N, Alessi MC, Dignat-George F, Vague IJ, Sampol J, Harle JR, et al. Does the anti-prothrombin antibodies measurement provide additional information in patients with thrombosis? Immunobiology 2007;212:557-65. (Pubitemid 47109157)
29. Galli M, Luciani D, Bertolini G, Barbui T. Anti-beta 2-glyco-protein I, antiprothrombin antibodies, and the risk of thrombosis in the antiphospholipid syndrome. Blood 2003;102:2717-23. (Pubitemid 37248837)
30. Jaskowski TD, Wilson AR, Hill HR, Branch WD, Tebo AE. Autoantibodies against phosphatidylserine, prothrombin and phosphatidylserine-prothrombin complex: identical or distinct diagnostic tools for antiphospholipid syndrome? Clin Chim Acta 2009;410:19-24.
31. Tincani A, Morozzi G, Afeltra A, Alessandri C, Allegri F, Bistoni O, et al. Antiprothrombin antibodies: a comparative analysis of homemade and commercial methods. A collaborative study by the Forum Interdisciplinare per la Ricerca nelle Malat-tie Autoimmuni (FIRMA). Clin Exp Rheumatol 2007;25:268-74. (Pubitemid 46851430)
32. Tsutsumi A, Hayashi T, Chino Y, Mamura M, Goto D, Matsumoto I, et al. Significance of antiprothrombin antibodies in patients with systemic lupus erythematosus: clinical evaluation of the antiprothrombin assay and the antiphosphatidylserine/prothrombin assay, and comparison with other antiphospholipid antibody assays. Mod Rheumatol 2006;16:158-64. (Pubitemid 43871691)
33. Oku K, Atsumi T, Amengual O, Koike T. Antiprothrombin antibody testing: detection and clinical utility. Semin Thromb Hemost 2008;34:335-9.
34. Ghirardello A, Bizzaro N, Zampieri S, Iaccarino L, Bassi N, Tozzoli R, et al. Biological and clinical relevance of anti-pro-thrombin antibodies. Ann N Y Acad Sci 2007;1109:503-10. (Pubitemid 47511393)
35. Bozic B, Cucnik S, Gaspersic N, Ambrozic A, Kveder T, Rozman B. Rational laboratory diagnostics of antiphospholipid antibodies: anti-cardiolipin, anti-β2-glycoprotein I, anti-pro-thrombin and anti-annexin v antibodies. J Int Fed Clin Chem 2010;20:1-8.