Global gene disruption in human cells to assign genes to phenotypes by deep sequencing

Nature Biotechnology

Volumn 29, Issue 6, 2011, Pages 542-546

  Carette, Jan E ^a,d   Guimaraes, Carla P ^a   Wuethrich, Irene ^a   Blomen, Vincent A ^a,d,e   Varadarajan, Malini ^a   Sun, Chong ^a   Bell, George ^a   Yuan, Bingbing ^a   Muellner, Markus K ^b   Nijman, Sebastian M ^b   Ploegh, Hidde L ^a,c   Brummelkamp, Thijn R ^a,d,e  

^a WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^b ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^c MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^d STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e NETHERLANDS CANCER INSTITUTE   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

GENE DISRUPTIONS; GENE FUNCTION; GRAM-NEGATIVE BACTERIA; HUMAN CANCER CELLS; HUMAN CELLS; HUMAN GENES; INSERTIONAL MUTAGENESIS; MUTANT ALLELES; PARALLEL SEQUENCING; RAPID ASSESSMENT; TISSUE DAMAGE; VIRULENCE FACTORS;

BACTERIA; CELL CULTURE; CLONE CELLS; LAKES; TISSUE;

GENES;

5 AZA 2' DEOXYCYTIDINE; CYTOLETHAL DISTENDING TOXIN; HOST FACTOR; IMATINIB; VIRULENCE FACTOR;

AGGREGATIBACTER ACTINOMYCETEMCOMITANS; ARTICLE; CAMPYLOBACTER JEJUNI; CELL CYCLE ARREST; CELL CYCLE G2 PHASE; CELL CYCLE M PHASE; CRYSTAL STRUCTURE; ESCHERICHIA COLI; EXON; GENE DISRUPTION; GENE INSERTION; HAEMOPHILUS DUCREYI; HUMAN; HUMAN CELL; MUTAGENESIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; TISSUE INJURY;

ALLELES; BACTERIAL PROTEINS; BACTERIAL TOXINS; CELL LINE, TUMOR; CHROMOSOMES, HUMAN; CLONING, MOLECULAR; GENETIC ASSOCIATION STUDIES; GENOME, HUMAN; GRAM-NEGATIVE BACTERIA; HAPLOIDY; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MUTAGENESIS, INSERTIONAL; MUTATION; PHENOTYPE; RNA INTERFERENCE; SEQUENCE TAGGED SITES; VIRULENCE FACTORS;

NEGIBACTERIA;

EID: 79958114328     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.1857     Document Type: Article

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