GC-TOFMS analysis of metabolites in adherent MDCK cells and a novel strategy for identifying intracellular metabolic markers for use as cell amount indicators in data normalization

Analytical and Bioanalytical Chemistry

Volumn 400, Issue 9, 2011, Pages 2983-2993

  Cao, Bei ^a   Aa, Jiye ^a   Wang, Guangji ^a   Wu, Xiaolan ^a   Liu, Linsheng ^a   Li, Mengjie ^a   Shi, Jian ^a   Wang, Xinwen ^a   Zhao, Chunyan ^a   Zheng, Tian ^a   Guo, Sheng ^b   Duan, Jinao ^b  

^a CHINA PHARMACEUTICAL UNIVERSITY   (China)

^b NANJING UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

Cell amount indicator; GC TOFMS; Inositol; Metabolic markers

Indexed keywords

ADHERENT CELLS; ALTERNATIVE APPROACH; BIOMEDICAL RESEARCH; CELL COUNTING; CELL LINES; CORRELATION COEFFICIENT; CULTURED CELL; DATA NORMALIZATION; GC-TOFMS; INOSITOL; INTRACELLULAR METABOLISM; INTRACELLULAR METABOLITES; MDCK CELLS; METABOLIC MARKERS; METABOLOMIC STUDY; NOVEL STRATEGIES; PRINCIPAL COMPONENTS ANALYSIS; PROTEIN ASSAYS; PROTEIN CONTENTS; RELATIVE STANDARD DEVIATIONS; SIGNAL INTENSITIES; UNDERLYING MECHANISM;

ALCOHOLS; BIOMOLECULES; CELL CULTURE; GAS CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; METABOLITES; PRINCIPAL COMPONENT ANALYSIS; PROTEINS; SUGARS;

CELLS;

GLYCOPROTEIN P;

ANIMAL; ARTICLE; CELL ADHESION; CELL LINE; DOG; EVALUATION; GENETIC TRANSFECTION; GENETICS; HUMAN; MASS FRAGMENTOGRAPHY; METABOLOME; METABOLOMICS; METHODOLOGY;

ANIMALS; CELL ADHESION; CELL LINE; DOGS; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; METABOLOME; METABOLOMICS; P-GLYCOPROTEIN; TRANSFECTION;

EID: 79958080469     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-011-4981-8     Document Type: Article

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