Quantification of heteroplasmic mitochondrial DNA mutations for DNA samples in the low picogram range by nested real-time ARMS-qPCR

Diagnostic Molecular Pathology

Volumn 20, Issue 2, 2011, Pages 117-122

  Biffi, Stefania ^a,b   Bortot, Barbara ^a   Carrozzi, Marco ^a   Severini, Giovanni Maria ^a  

^a INSTITUTE FOR MATERNAL AND CHILD HEALTH IRCCS BURLO GAROFOLO   (Italy)

^b Optical Imaging Laboratory   (Italy)

Author keywords

ARMS PCR; heteroplasmy; mitochondrial DNA; nested real time PCR; single fiber PCR; SYBR Green

Indexed keywords

MITOCHONDRIAL DNA;

ARTICLE; CASE REPORT; CHILD; DISORDERS OF MITOCHONDRIAL FUNCTIONS; GENE DOSAGE; GENE MUTATION; GENETIC ANALYSIS; HETEROPLASMY; HUMAN; LIMIT OF DETECTION; NESTED REAL TIME AMPLIFICATION REFRACTORY MUTATION SYSTEM QUANTITATIVE POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SCHOOL CHILD;

CHILD; DNA, MITOCHONDRIAL; HUMANS; MITOCHONDRIAL DISEASES; MUTATION; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 79958041835     PISSN: 10529551     EISSN: 15334066     Source Type: Journal    
DOI: 10.1097/PDM.0b013e3181efe2c6     Document Type: Article

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