Separation and pseudo-coloring of high-speed bright-field microscopy images of the beating embryonic heart

Conference Record - Asilomar Conference on Signals, Systems and Computers

Volumn , Issue , 2010, Pages 337-340

  Bhat, Sandeep ^a   Liebling, Michael ^a  

^a University of California   (United States)

Author keywords

Bright field microscopy; fluorescence microscopy; multi channel imaging; source separation; visualization

Indexed keywords

BRIGHT-FIELD MICROSCOPY; BRIGHTFIELD; CARDIAC STRUCTURE; CARDIAC TISSUES; HEART FUNCTION; HEART WALL; HIGH-SPEED; IMAGE CONTRASTS; MOTION PATTERN; MULTICHANNEL IMAGING; QUANTITATIVE CHARACTERIZATION; RED BLOOD CELL; SOURCE SEPARATION; STATIC BACKGROUND; ZEBRAFISH;

BLOOD; FLUORESCENCE MICROSCOPY; TISSUE; VISUALIZATION;

HEART;

EID: 79958013772     PISSN: 10586393     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1109/ACSSC.2010.5757530     Document Type: Conference Paper

References (15)

1. J. Vermot, A. S. Forouhar, M. Liebling, D. Wu, D. Plummer, M. Gharib, and S. E. Fraser, "Reversing blood flows act through klf2a to ensure normal valvulogenesis in the developing heart," PLoS Biol, vol. 7, no. 11, p. e1000246, Nov 2009.
2. J. Vermot, S. E. Fraser, and M. Liebling, "Fast fluorescence microscopy for imaging the dynamics of embryonic development," HFSP Journal, vol. 2, pp. 143-155, 2008.
3. R. Ali, M. Gooding, M. Christlieb, and M. Brady, "Advanced phase-based segmentation of multiple cells from brightfield microscopy images," Proc. ISBI 2008, pp. 181-184, May 2008.
4. M. Tscherepanow, F. Zöllner, M. Hillebrand, and F. Kummert, "Automatic segmentation of unstained living cells in bright-field microscope images," Advances in Mass Data Analysis of Images and Signals in Medicine, Biotechnology, Chemistry and Food Industry, pp. 158-172, 2008.
5. M. Iwanowski and A. Korzyńska, "Segmentation of moving cells in bright field and epi-fluorescent microscopic image sequences," Computer Vision and Graphics, pp. 401-410, 2010.
6. E. Be'ery and A. Yeredor, "Blind separation of superimposed shifted images using parameterized joint diagonalization," IEEE Trans. Image Process., vol. 17, no. 3, pp. 340-353, 2008.
7. P. Kisilev, M. Zibtulevsky, and Y. Zeevi, "Blind separation of mixed images using multiscale transforms," Proc. ICIP 2003, vol. 1, pp. 990-997, 2003.
8. A. Goshtasby and D. Turner, "Segmentation of cardiac cine MR images for extraction of right and left ventricular chambers," IEEE Trans. Med. Imag., vol. 14, no. 1, pp. 56-64, mar. 1995.
9. U. Kurkure, A. Pednekar, R. Muthupillai, S. Flamm, and I. Kakadiaris, "Localization and segmentation of left ventricle in cardiac cine-MR images," IEEE Trans. Biomed. Eng., vol. 56, no. 5, pp. 1360-1370, may. 2009.
10. H. Zhang, A. Wahle, R. Johnson, T. Scholz, and M. Sonka, "4-D cardiac MR image analysis: Left and right ventricular morphology and function," IEEE Trans. Med. Imag., vol. 29, no. 2, pp. 350-364, feb. 2010.
11. J. Bosch, S. Mitchell, B. Lelieveldt, F. Nijland, O. Kamp, M. Sonka, and J. Reiber, "Automatic segmentation of echocardiographic sequences by active appearance motion models," IEEE Trans. Med. Imag., vol. 21, no. 11, pp. 1374-1383, Nov. 2002.
12. S. Mitchell, J. Bosch, B. Lelieveldt, R. van der Geest, J. Reiber, and M. Sonka, "3-D active appearance models: segmentation of cardiac MR and ultrasound images," IEEE Trans. Med. Imag., vol. 21, no. 9, pp. 1167-1178, sep. 2002.
13. S. Bhat and M. Liebling, "Cardiac tissue and erythrocyte separation in bright-field microscopy images of the embryonic zebrafish heart for motion estimation," in Proc. ISBI 2009, June 28-July 1 2009, pp. 746-749.
14. S. Bhat, I. Larina, K. Larin, M. Dickinson, and M. Liebling, "Multiple cardiac cycle noise reduction in dynamic optical coherence tomography of the embryonic heart and vasculature," Opt. Letters, vol. 34, no. 23, pp. 3704-3706, Dec 2009.
15. Bitplane, "Imaris," 3D and 4D Real-Time Interactive Data Visualization Software, 1992-2010, available: http://www.bitplane.com/go/ products/imaris.