Isolation and characterization of Orientia tsutsugamushi from rodents captured following a scrub typhus outbreak at a military training base, Bothong district, Chonburi province, central Thailand

American Journal of Tropical Medicine and Hygiene

Volumn 84, Issue 4, 2011, Pages 599-607

  Rodkvamtook, Wuttikon ^a   Ruang areerate, Toon ^a   Gaywee, Jariyanart ^a   Richards, Allen L ^b   Jeamwattanalert, Pimmada ^a   Bodhidatta, Dharadhida ^a   Sangjun, Noppadon ^a   Prasartvit, Anchana ^c   Jatisatienr, Araya ^d   Jatisatienr, Chaiwat ^d  

^a ARMED FORCES RESEARCH INSTITUTE OF MEDICAL SCIENCES   (Thailand)

^b NAVAL MEDICAL RESEARCH CENTER   (United States)

^c MINISTRY OF PUBLIC HEALTH   (Thailand)

^d CHIANG MAI UNIVERSITY   (Thailand)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM ANTIBODY; GENOMIC DNA; IMMUNOGLOBULIN G ANTIBODY; IMMUNOGLOBULIN M ANTIBODY; RNA 16S;

ADULT; ANIMAL CELL; ANIMAL TISSUE; ANTIBODY DETECTION; ANTIBODY TITER; ARMY; ARTICLE; ASCHOSCHOENGASTIA; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BANDICOTA INDICA; BANDICOTA SAVILEI; CLINICAL ARTICLE; CONTROLLED STUDY; DISEASE CARRIER; DISEASE TRANSMISSION; DNA SEQUENCE; EPIDEMIC; FEMALE; FIBROBLAST CULTURE; FLUORESCENT ANTIBODY TECHNIQUE; GAHRLIEPIA; HUMAN; MALE; MENESTES BERDMOREI; MOUSE; MURIDAE; MUS CAROLI; NONHUMAN; NUCLEOTIDE SEQUENCE; ORIENTIA TSUTSUGAMUSHI; PHYLOGENETIC TREE; RAT; RATTUS EXULANDS; RATTUS LOSEA; RATTUS RATTUS; RODENT; SCANDENTIA; SCRUB TYPHUS; THAILAND; TROMBICULA AKUMUSHI; TROMBICULA DELIENSIS; TROMBICULIDAE; TUPAIA GLIS; ANIMAL; GENETICS; MICROBIOLOGY; MIDDLE AGED; PHYLOGENY; SOLDIER; ZOONOSIS;

ACARI; NEGIBACTERIA; ORIENTIA TSUTSUGAMUSHI; RODENTIA; TROMBICULIDAE;

ADULT; ANIMALS; DISEASE OUTBREAKS; FEMALE; HUMANS; MALE; MIDDLE AGED; MILITARY PERSONNEL; ORIENTIA TSUTSUGAMUSHI; PHYLOGENY; RODENTIA; SCRUB TYPHUS; THAILAND; YOUNG ADULT; ZOONOSES;

EID: 79958007108     PISSN: 00029637     EISSN: None     Source Type: Journal    
DOI: 10.4269/ajtmh.2011.09-0768     Document Type: Article

References (41)

1. Philip CB, 1948. Tsutsugamushi disease in World War II. J Parasitol 34: 169-191.
2. Ohashi N, Fukuhara M, Shimada M, Tamura A, 1995. Phylogenetic position of Rickettsia tsutsugamushi and the relationship among its antigenic variants by analyses of 16S rRNA gene sequences. FEMS Microbiol Lett 125: 299-304.
3. Watt G, Parola P, 2003. Scrub typhus and tropical rickettsioses. Curr Opin Infect Dis 16: 429-436. (Pubitemid 37270602)
4. Tamura A, Ohashi N, Urakami H, Miyamura S, 1995. Classification of Rickettsia tsutsugamushi in a new genus, Orientia gen. nov., as Orientia tsutsugamushi comb. nov. Int J Syst Bacteriol 45: 589-591.
5. Tamura A, Urakami H, Ohashi N, 1991. A comparative view of Rickettsia tsutsugamushi and the other groups of rickettsiae. Eur J Epidemiol 7: 259-269.
6. Kawamura AJ, Tanaka H, Tamura A, Kawamura AJ, Tanaka H, Tamura A, 1995. Tsutsugamushi Disease. Tokyo, Japan: University of Tokyo Press.
7. Seong SY, Choi MS, Kim IS, 2001. Orientia tsutsugamushi infection: overview and immune responses. Microbes Infect 3: 11-21.
8. Shirai A, Wisseman CL Jr, 1975. Serologic classification of scrub typhus isolates from Pakistan. Am J Trop Med Hyg 24: 145-153.
9. Currie B, 1993. Medicine in tropical Australia. Med J Aust 158: 609, 612-615.
10. Graves S, Wang L, Nack Z, Jones S, 1999. Rickettsia serosurvey in Kimberley, western Australia. Am J Trop Med Hyg 60: 786-789. (Pubitemid 29233082)
11. Ogawa M, Hagiwara T, Kishimoto T, Shiga S, Yoshida Y, Furuya Y, Kaiho I, Ito T, Nemoto H, Yamamoto N, Masukawa K, 2002. Scrub typhus in Japan: epidemiology and clinical features of cases reported in 1998. Am J Trop Med Hyg 67: 162-165.
12. Chong Y, 1989. Application of serologic diagnosis of tsutsugamushi disease (scrub typhus) in Korea where the disease was recently recognized to be endemic. Yonsei Med J 30: 111-117. (Pubitemid 19231885)
13. Yi KS, Chong Y, Covington SC, Donahue BJ, Rothen RL, Rodriguez J, Arthur JD, 1993. Scrub typhus in Korea: importance of early clinical diagnosis in this newly recognized endemic area. Mil Med 158: 269-273. (Pubitemid 23115693)
14. Kollars TM Jr, Bodhidatta D, Phulsuksombati D, Tippayachai B, Coleman RE, 2003. Short report: variation in the 56-kD type-specific antigen gene of Orientia tsutsugamushi isolated from patients in Thailand. Am J Trop Med Hyg 68: 299-300. (Pubitemid 38240516)
15. Singhsilarak T, Leowattana W, Looareesuwan S, Wongchotigul V, Jiang J, Richards AL, Watt G, 2005. Short report: detection of Orientia tsutsugamushi in clinical samples by quantitative real-time polymerase chain reaction. Am J Trop Med Hyg 72: 640-641. (Pubitemid 41278837)
16. Chattopadhyay S, Richards AL, 2007. Scrub typhus vaccines: past history and recent developments. Hum Vaccin 3: 73-80. (Pubitemid 46717371)
17. Kelly DJ, Richards AL, Temenak J, Strickman D, Dasch GA, 2002. The past and present threat of rickettsial diseases to military medicine and international public health. Clin Infect Dis 34: S145-S169. (Pubitemid 34618507)
18. Shishido A, 1962. Identification and serolgical classification of the causative agent of scrub typhus in Japan. Jpn J Sci Biol 15: 308-321.
19. Eisemann CS, Osterman JV, 1981. Antigens of scrub typhus rickettsiae: separation by polyacrylamide gel electrophoresis and identification by enzyme-linked immunosorbent assay. Infect Immun 32: 525-533. (Pubitemid 11101679)
20. Tamura A, Ohashi N, Koyama Y, Fukuhara M, Kawamori F, Otsuru M, Wu PF, Lin SY, 1997. Characterization of Orientia tsutsugamushi isolated in Taiwan by immunofluorescence and restriction fragment length polymorphism analyses. FEMS Microbiol Lett 150: 225-231. (Pubitemid 27241555)
21. Elisberg BL, Campbell JM, Bozeman FM, 1968. Antigenic diversity of Rickettsia tsutsugamushi: epidemiologic and ecologic significance. J Hyg Epidemiol Microbiol Immunol 12: 18-25.
22. Tamura A, Takahashi K, Tsuruhara T, Urakami H, Miyamura S, Sekikawa H, Kenmotsu M, Shibata M, Abe S, Nezu H, 1984. Isolation of Rickettsia tsutsugamushi antigenically different from Kato, Karp, and Gilliam strains from patients. Microbiol Immunol 28: 873-882. (Pubitemid 14030254)
23. Qiang Y, Tamura A, Urakami H, Makisaka Y, Koyama S, Fukuhara M, Kadosaka T, 2003. Phylogenetic characterization of Orientia tsutsugamushi isolated in Taiwan according to the sequence homologies of 56-kDa type-specific antigen genes. Microbiol Immunol 47: 577-583. (Pubitemid 37010385)
24. Tay ST, Rohani YM, Ho TM, Shamala D, 2005. Sequence analysis of the hypervariable regions of the 56-kDa immunodominant protein genes of Orientia tsutsugamushi strains in Malaysia. Microbiol Immunol 49: 67-71. (Pubitemid 40313121)
25. Ohashi N, Koyama Y, Urakami H, Fukuhara M, Tamura A, Kawamori F, Yamamoto S, Kasuya S, Yoshimura K, 1996. Demonstration of antigenic and genotypic variation in Orientia tsutsugamushi which were isolated in Japan, and their classification into type and subtype. Microbiol Immunol 40: 627-638. (Pubitemid 26327222)
26. Enatsu T, Urakami H, Tamura A, 1999. Phylogenetic analysis of Orientia tsutsugamushi strains based on the sequence homologies of 56-kDa type-specific antigen genes. FEMS Microbiol Lett 180: 163-169. (Pubitemid 29507562)
27. Kelly DJ, Fuerst PA, Ching WM, Richards AL, 2009. Scrub typhus: the geographical distribution of phenotypic and genotypic variants of Orientia tsutsugamushi. Clin Infect Dis 48: S203-S230.
28. Bozeman FM, Elisberg BL, 1963. Serological diagnosis of scrub typhus by indirect immunofluorescence. Proc Soc Exp Biol Med 112: 568-573.
29. Robinson DM, Brown G, Gan E, Huxsoll DL, 1976. Adaptation of a microimmunofluorescence test to the study of human Rickettsia tsutsugamushi antibody. Am J Trop Med Hyg 25: 900-905. (Pubitemid 8001569)
30. Russell E, Monkanna T, Kenneth KJ, Strickman DJ, Frances SP, Tanskul P, Kollars TM, Inkaminlao JR, Watcharapichat P, Khlaimanee N, Phulsuksombati D, Sangjun N, Lerdthusnee K, 2003. Occurrence of Orientia tsutsugamushi in small mammals from Thailand. Am J Trop Med Hyg 69: 519-524. (Pubitemid 38248253)
31. Lerdthusnee K, Khuntirat B, Leepitakrat W, Tanskul P, Monkanna T, Khlaimanee N, Inlao I, Kengluecha A, Mungviriya S, Chandranoi K, Krairojananan P, Bodhidatta D, Rodkwamthook W, Phulsuksombati D, Sangjun N, Watcharapichat P, Jones JW, Coleman RE, 2003. Scrub typhus vector competence of Leptotrombidium chiangraiensis chiggers and transmission efficacy and isolation of Orientia tsutsugamushi. Ann N Y Acad Sci 990: 25-35. (Pubitemid 36808276)
32. Clark DC, Bladwin RL, Bayne KA, Brown MJ, Gebhart GF, Gonder JC, Gwathmey JK, Keeling ME, Kohn DF, Robb JW, Smith OA, Steggerda JA, Vandenbergh JG, White WJ, Williams-Blangero S, VandeBerg JL, 1996. Guide for the Care and Use of Laboratory Animals. Washington, DC: National Academy Press.
33. O'Neill SL, Giordano R, Colbert AM, Karr TL, Robertson HM, 1992. 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc Natl Acad Sci USA 89: 2699-2702.
34. Swofford DL, 2002. PAUP*. Phylogenetic analysis using pasimony (* and other methods). Version 4.0 beta 10. Sunderland, MA: Sinaneur Associates.
35. Posada D, 2003. Using MODELTEST and PAUP* to select a modezl of nucleotide substitution. Curr Protoc Bioinformatics Chapter 6: Unit 6.5.
36. Posada D, Crandall KA, 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics 14: 817-818. (Pubitemid 28552116)
37. Richards AL, Soeatmandji DW, Widodo MA, Sardjono TW, Yanuwiadi B, Hernowati TE, Baskoro AD, Roebiyoso, Hakim L, Soendoro M, Rahardjo E, Putri MP, Saragih JM, Strickman D, Kelly DJ, Dasch GA, Olson JG, Church CJ, Corwin AL, 1997. Seroepidemiological evidence for murine and scrub typhus in Malang, Indonesia. Am J Trop Med Hyg 57: 91-95.
38. Frances SP, Watchrapichat P, Phunlsuksombati D, 2000. Development and persistence of antobodies to Orientia tsutsugamushi in the roof rat, Rattus rattus and laboratory mice following attachment of naturally infected Leptotrombidium deliense. Acta Trop 77: 279-285.
39. Jiang J, Sangkasuwan V, Lerdthusnee K, Sukwit S, Chuenchitra T, Rozmajzl PJ, Eamsila C, Jones JW, Richards AL, 2005. Molecular evidence of human infection with TT-118, Rickettsia honei, from Thailand. Emerg Infect Dis 11: 1475-1477.
40. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ, 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-3402. (Pubitemid 27359211)
41. Tamura A, Ohashi N, Urakami H, Takahashi K, Oyanagi M, 1985. Analysis of polypeptide composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting. Infect Immun 48: 671-675. (Pubitemid 15021313)