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Volumn 57, Issue , 2011, Pages 47-61

Isolation of synapse subdomains by subcellular fractionation using sucrose density gradient centrifugation

Author keywords

Detergent insoluble cytoskeleton; Detergent insoluble membrane; Postsynaptic density; Subcellular fractionation; Synaptic plasma membrane; Synaptosome

Indexed keywords

DETERGENT;

EID: 79957765006     PISSN: 08932336     EISSN: 19406045     Source Type: Book Series    
DOI: 10.1007/978-1-61779-111-6_4     Document Type: Article
Times cited : (9)

References (41)
  • 1
    • 0021242835 scopus 로고
    • The effects of detergents on the composition of postsynaptic densities
    • Somerville, R. A., Merz, P. A., and Carp, R. I. (1984) The effects of detergents on the com¬position of postsynaptic densities, J Neurochem 43, 184-191. (Pubitemid 14083333)
    • (1984) Journal of Neurochemistry , vol.43 , Issue.1 , pp. 184-191
    • Somerville, R.A.1    Merz, P.A.2    Carp, R.I.3
  • 2
    • 0017396421 scopus 로고
    • The structure of postsynaptic densities isolated from dog cerebral cortex. I. Overall morphology and protein composition
    • Cohen, R. S., Blomberg, F., Berzins, K., and Siekevitz, P. (1977) The structure of postsyn-aptic densities isolated from dog cerebral cor¬tex. I. Overall morphology and protein composition, J Cell Biol 74, 181-203. (Pubitemid 8141493)
    • (1977) Journal of Cell Biology , vol.74 , Issue.1 , pp. 181-203
    • Cohen, R.S.1    Blomberg, F.2    Berzins, K.3    Siekevitz, P.4
  • 3
    • 0022559882 scopus 로고
    • 3H]glutamate to fresh or frozen synaptic membrane and postsynaptic density fractions isolated from cerebral cortex and cerebellum of fresh or frozen canine brain
    • Wu, K., Carlin, R., and Siekevitz, P. (1986) Binding of L-[3H]glutamate to fresh or frozen synaptic membrane and postsynaptic density fractions isolated from cerebral cortex and cer¬ebellum of fresh or frozen canine brain, J Neurochem 46, 831-841. (Pubitemid 16114399)
    • (1986) Journal of Neurochemistry , vol.46 , Issue.3 , pp. 831-841
    • Wu, K.1    Carlin, R.2    Siekevitz, P.3
  • 4
    • 0019134836 scopus 로고
    • Isolation and characteriza¬tion of postsynaptic densities from various brain regions: Enrichment of different types of postsynaptic densities
    • Carlin, R. K., Grab, D. J., Cohen, R. S., and Siekevitz, P. (1980) Isolation and characteriza¬tion of postsynaptic densities from various brain regions: enrichment of different types of postsynaptic densities, J Cell Biol 86, 831-845.
    • (1980) J Cell Biol , vol.86 , pp. 831-845
    • Carlin, R.K.1    Grab, D.J.2    Cohen, R.S.3    Siekevitz, P.4
  • 5
    • 0029112093 scopus 로고
    • Defciency of brain synaptic dystrophin in human Duchenne muscular dystrophy
    • Kim, T. W., Wu, K., and Black, I. B. (1995) Defciency of brain synaptic dystrophin in human Duchenne muscular dystrophy, Ann Neurol 38, 446-449.
    • (1995) Ann Neurol , vol.38 , pp. 446-449
    • Kim, T.W.1    Wu, K.2    Black, I.B.3
  • 7
    • 0027293091 scopus 로고
    • Characterization of protein kinase C activities in postsynaptic density fractions prepared from cerebral cortex, hippocampus, and cerebellum
    • Suzuki, T., Okumura-Noji, K., Tanaka, R., Ogura, A., Nakamura, K., Kudo, Y., and Tada, T. (1993) Characterization of protein kinase C activities in postsynaptic density fractions prepared from cerebral cortex, hippocampus, and cerebellum, Brain Res 619, 69-75. (Pubitemid 23214306)
    • (1993) Brain Research , vol.619 , Issue.1-2 , pp. 69-75
    • Suzuki, T.1    Okumura-Noji, K.2    Tanaka, R.3    Ogura, A.4    Nakamura, K.5    Kudo, Y.6    Tada, T.7
  • 8
    • 0026474587 scopus 로고
    • Detection of dystrophin in the post-synaptic density of rat brain and defciency in a mouse model of Duchenne muscular dystro¬phy
    • Kim, T. W., Wu, K., Xu, J. L., and Black, I. B. (1992) Detection of dystrophin in the post-synaptic density of rat brain and defciency in a mouse model of Duchenne muscular dystro¬phy, Proc Natl Acad Sci USA 89, 11642-11644.
    • (1992) Proc Natl Acad Sci USA , vol.89 , pp. 11642-11644
    • Kim, T.W.1    Wu, K.2    Xu, J.L.3    Black, I.B.4
  • 10
    • 0023806990 scopus 로고
    • Neurochemical characteristics of a postsynaptic density frac¬tion isolated from adult canine hippocampus
    • Wu, K., and Siekevitz, P. (1988) Neurochemical characteristics of a postsynaptic density frac¬tion isolated from adult canine hippocampus, Brain Res 457, 98-112.
    • (1988) Brain Res , vol.457 , pp. 98-112
    • Wu, K.1    Siekevitz, P.2
  • 11
    • 0030869946 scopus 로고    scopus 로고
    • Excitable membranes and synaptic transmission: Postsynaptic mechanisms. Localization of α-internexin in the postsynaptic density of the rat brain
    • DOI 10.1016/S0006-8993(97)00492-7, PII S0006899397004927
    • Suzuki, T., Mitake, S., Okumura-Noji, K., Shimizu, H., Tada, T., and Fujii, T. (1997) Excitable membranes and synaptic transmis¬sion: postsynaptic mechanisms. Localization of alpha-internexin in the postsynaptic density of the rat brain, Brain Res 765, 74-80. (Pubitemid 27374916)
    • (1997) Brain Research , vol.765 , Issue.1 , pp. 74-80
    • Suzuki, T.1    Mitake, S.2    Okumura-Noji, K.3    Shimizu, H.4    Tada, T.5    Fujii, T.6
  • 12
    • 0019209573 scopus 로고
    • Brain postsynaptic densities: Their relationship to glial and neuronal filaments
    • DOI 10.1083/jcb.87.2.346
    • Matus, A., Pehling, G., Ackermann, M., and Maeder, J. (1980) Brain postsynaptic densities: the relationship to glial and neuronal flaments, J Cell Biol 87, 346-359. (Pubitemid 11204329)
    • (1980) Journal of Cell Biology , vol.87 , Issue.I2 , pp. 346-359
    • Matus, A.1    Pehling, G.2    Ackermann, M.3    Maeder, J.4
  • 13
    • 33846168749 scopus 로고    scopus 로고
    • Characterization of mRNA species that are associated with postsynaptic density fraction by gene chip microarray analysis
    • DOI 10.1016/j.neures.2006.09.009, PII S0168010206002458
    • Suzuki, T., Tian, Q. B., Kuromitsu, J., Kawai, T., and Endo, S. (2007) Characterization of mRNA species that are associated with post-synaptic density fraction by gene chip microar-ray analysis, Neurosci Res 57, 61-85. (Pubitemid 46084867)
    • (2007) Neuroscience Research , vol.57 , Issue.1 , pp. 61-85
    • Suzuki, T.1    Tian, Q.B.2    Kuromitsu, J.3    Kawai, T.4    Endo, S.5
  • 14
    • 0015461599 scopus 로고
    • Isolation and structural studies on synaptic complexes from rat brain
    • Cotman, C. W., and Taylor, D. (1972) Isolation and structural studies on synaptic complexes from rat brain, J Cell Biol 55, 696-711.
    • (1972) J Cell Biol , vol.55 , pp. 696-711
    • Cotman, C.W.1    Taylor, D.2
  • 15
    • 0019407312 scopus 로고
    • Optimal concentration of iodonitrotetrazolium for the isolation of junctional fractions from rat brain
    • DOI 10.1007/BF00964046
    • Nieto-Sampedro, M., Bussineau, C. M., and Cotman, C. W. (1981) Optimal concentration of iodonitrotetrazolium for the isolation of junctional fractions from rat brain, Neurochem Res 6, 307-320. (Pubitemid 11039743)
    • (1981) Neurochemical Research , vol.6 , Issue.3 , pp. 307-320
    • Nieto-Sampedro, M.1    Bussineau, C.M.2    Cotman, C.W.3
  • 16
    • 0016265495 scopus 로고
    • Isolation of postsynaptic densities from rat brain
    • Cotman, C. W., Banker, G., Churchill, L., and Taylor, D. (1974) Isolation of postsynaptic densities from rat brain, J Cell Biol 63, 441-455.
    • (1974) J Cell Biol , vol.63 , pp. 441-455
    • Cotman, C.W.1    Banker, G.2    Churchill, L.3    Taylor, D.4
  • 17
    • 0020034334 scopus 로고
    • Subcellular localization of the 52,000 molecular weight major postsynaptic density protein
    • DOI 10.1016/0006-8993(82)91202-1
    • Kelly, P. T., and Montgomery, P. R. (1982) Subcellular localization of the 52,000 molecu¬lar weight major postsynaptic density protein, Brain Res 233, 265-286. (Pubitemid 12162643)
    • (1982) Brain Research , vol.233 , Issue.2 , pp. 265-286
    • Kelly, P.T.1    Montgomery, P.R.2
  • 18
    • 0017041266 scopus 로고
    • Intermolecular disulfide bonds at central nervous system synaptic junctions
    • DOI 10.1016/0006-291X(76)90200-X
    • Kelly, P. T., and Cotman, C. W. (1976) Intermolecular disulfde bonds at central ner¬vous system synaptic junctions, Biochem Biophys Res Commun 73, 858-864. (Pubitemid 8004565)
    • (1976) Biochemical and Biophysical Research Communications , vol.73 , Issue.4 , pp. 858-864
    • Kelly, P.T.1    Cotman, C.W.2
  • 19
    • 0019349993 scopus 로고
    • Developmental changes in morphology and molecular composition of isolated synaptic junc-tional structures
    • Kelly, P. T., and Cotman, C. W. (1981) Developmental changes in morphology and molecular composition of isolated synaptic junc-tional structures, Brain Res 206, 251-257.
    • (1981) Brain Res , vol.206 , pp. 251-257
    • Kelly, P.T.1    Cotman, C.W.2
  • 20
    • 0032692291 scopus 로고    scopus 로고
    • Interprotein disulfde bonds formed during isolation process tighten the structure of the postsynaptic density
    • Lai, S. L., Chiang, S. F., Chen, I. T., Chow, W. Y., and Chang, Y. C. (1999) Interprotein disulfde bonds formed during isolation process tighten the structure of the postsynaptic density, J Neurochem 73, 2130-2138.
    • (1999) J Neurochem , vol.73 , pp. 2130-2138
    • Lai, S.L.1    Chiang, S.F.2    Chen, I.T.3    Chow, W.Y.4    Chang, Y.C.5
  • 21
    • 0343526821 scopus 로고    scopus 로고
    • Effects of disulfde bonds formed dur¬ing isolation process on the structure of the postsynaptic density
    • Sui, C. W., Chow, W. Y., and Chang, Y. C. (2000) Effects of disulfde bonds formed dur¬ing isolation process on the structure of the postsynaptic density, Brain Res 873, 268-273.
    • (2000) Brain Res , vol.873 , pp. 268-273
    • Sui, C.W.1    Chow, W.Y.2    Chang, Y.C.3
  • 22
    • 0028111814 scopus 로고
    • 2+/calmodulin-dependent protein kinase II into postsynaptic density after decapitation
    • 2+/calmodulin-dependent protein kinase II into postsynaptic density after decapitation, J Neurochem 63, 1529-1537.
    • (1994) J Neurochem , vol.63 , pp. 1529-1537
    • Suzuki, T.1    Okumura-Noji, K.2    Tanaka, R.3    Tada, T.4
  • 23
    • 0020059402 scopus 로고
    • Postmortem accumulation of tubulin in postsynaptic density preparations
    • Carlin, R. K., Grab, D. J., and Siekevitz, P. (1982) Postmortem accumulation of tubulin in postsynaptic density preparations, J Neurochem 38, 94-100. (Pubitemid 12189199)
    • (1982) Journal of Neurochemistry , vol.38 , Issue.1 , pp. 94-100
    • Carlin, R.K.1    Grab, D.J.2    Siekevitz, P.3
  • 24
    • 62849098808 scopus 로고    scopus 로고
    • Cold-induced exodus of postsynaptic proteins from dendritic spines
    • Cheng, H. H., Huang, Z. H., Lin, W. H., Chow, W. Y., and Chang, Y. C. (2009) Cold-induced exodus of postsynaptic proteins from dendritic spines, J Neurosci Res 87, 460-469.
    • (2009) J Neurosci Res , vol.87 , pp. 460-469
    • Cheng, H.H.1    Huang, Z.H.2    Lin, W.H.3    Chow, W.Y.4    Chang, Y.C.5
  • 25
    • 34547520352 scopus 로고    scopus 로고
    • Two pools of Triton X-100-insoluble GABAA receptors are present in the brain, one associated to lipid rafts and another one to the post-synaptic GABAergic complex
    • DOI 10.1111/j.1471-4159.2007.04635.x
    • Li, X., Serwanski, D. R., Miralles, C. P., Bahr, B. A., and De Blas, A. L. (2007) Two pools of Triton X-100-insoluble GABA(A) receptors are present in the brain, one associated to lipid rafts and another one to the post-synaptic GABAergic complex, J Neurochem 102, 1329-1345. (Pubitemid 47179808)
    • (2007) Journal of Neurochemistry , vol.102 , Issue.4 , pp. 1329-1345
    • Li, X.1    Serwanski, D.R.2    Miralles, C.P.3    Bahr, B.A.4    De Blas, A.L.5
  • 26
    • 0020608651 scopus 로고
    • Isolation of postsynaptic densities retaining their membrane attachment
    • DOI 10.1016/0306-4522(83)90179-3
    • Ratner, N., and Mahler, H. (1983) Isolation of postsynaptic densities retaining their mem¬brane attachment, Neuroscience 9, 631-644. (Pubitemid 13035013)
    • (1983) Neuroscience , vol.9 , Issue.3 , pp. 631-644
    • Ratner, N.1    Mahler, H.2
  • 27
    • 0026492629 scopus 로고
    • The rat brain postsynaptic density frac¬tion contains a homolog of the Drosophila discs-large tumor suppressor protein
    • Cho, K. O., Hunt, C. A., and Kennedy, M. B. (1992) The rat brain postsynaptic density frac¬tion contains a homolog of the Drosophila discs-large tumor suppressor protein, Neuron 9, 929-942.
    • (1992) Neuron , vol.9 , pp. 929-942
    • Cho, K.O.1    Hunt, C.A.2    Kennedy, M.B.3
  • 29
    • 33750486875 scopus 로고    scopus 로고
    • BRAG1, a Sec7 domain-containing protein, is a component of the postsynaptic density of excitatory synapses
    • DOI 10.1016/j.brainres.2006.08.096, PII S0006899306025893
    • Murphy, J. A., Jensen, O. N., and Walikonis, R. S. (2006) BRAG1, a Sec7 domain-containing protein, is a component of the postsynaptic density of excitatory synapses, Brain Res 1120, 35-45. (Pubitemid 44648014)
    • (2006) Brain Research , vol.1120 , Issue.1 , pp. 35-45
    • Murphy, J.A.1    Jensen, O.N.2    Walikonis, R.S.3
  • 30
    • 0036768289 scopus 로고    scopus 로고
    • Lipid rafts at postsynaptic sites: Distribution, function and linkage to postsynaptic density
    • DOI 10.1016/S0168-0102(02)00080-9, PII S0168010202000809
    • Suzuki, T. (2002) Lipid rafts at postsynaptic sites: distribution, function and linkage to postsynaptic density, Neurosci Res 44, 1-9. (Pubitemid 36158698)
    • (2002) Neuroscience Research , vol.44 , Issue.1 , pp. 1-9
    • Suzuki, T.1
  • 31
    • 0017408222 scopus 로고
    • The structure of postsynaptic densities isolated from dog cerebral cortex. II. Characterization and arrangement of some of the major proteins within the structure
    • Blomberg, F., Cohen, R. S., and Siekevitz, P. (1977) The structure of postsynaptic densities isolated from dog cerebral cortex. II. Characterization and arrangement of some of the major proteins within the structure, J Cell Biol 74, 204-225. (Pubitemid 8141494)
    • (1977) Journal of Cell Biology , vol.74 , Issue.1 , pp. 204-225
    • Blomberg, F.1    Cohen, R.C.2    Siekevitz, P.3
  • 33
    • 0020406690 scopus 로고
    • Biochemical and morphological comparison of postsynaptic densities prepared from rat, hamster, and monkey brains by phase partitioning
    • DOI 10.1111/j.1471-4159.1982.tb11504.x
    • Gurd, J. W., Gordon-Weeks, P., and Evans, W. H. (1982) Biochemical and morphological comparison of postsynaptic densities prepared from rat, hamster, and monkey brains by phase partitioning, J Neurochem 39, 1117-1124. (Pubitemid 13200035)
    • (1982) Journal of Neurochemistry , vol.39 , Issue.4 , pp. 1117-1124
    • Gurd, J.W.1    Gordon-Weeks, P.2    Evans, W.H.3
  • 34
    • 3543055474 scopus 로고
    • The postsynaptic density
    • Matus, A. (1981) The postsynaptic density, Trends Neurosci 4, 51-53.
    • (1981) Trends Neurosci , vol.4 , pp. 51-53
    • Matus, A.1
  • 35
    • 38949168914 scopus 로고    scopus 로고
    • Visualization of detergent solubili-zation of membranes: Implications for the iso¬lation of rafts
    • Garner, A. E., Smith, D. A., and Hooper, N. M. (2008) Visualization of detergent solubili-zation of membranes: implications for the iso¬lation of rafts, Biophys J 94, 1326-1340.
    • (2008) Biophys J , vol.94 , pp. 1326-1340
    • Garner, A.E.1    Smith, D.A.2    Hooper, N.M.3
  • 36
    • 0141828502 scopus 로고    scopus 로고
    • Use of detergents to study membrane rafts: The good, the bad, and the ugly
    • DOI 10.1515/BC.2003.139
    • Shogomori, H., and Brown, D. A. (2003) Use of detergents to study membrane rafts: the good, the bad, and the ugly, Biol Chem 384, 1259-1263. (Pubitemid 37168692)
    • (2003) Biological Chemistry , vol.384 , Issue.9 , pp. 1259-1263
    • Shogomori, H.1    Brown, D.A.2
  • 38
    • 0019012370 scopus 로고
    • Pitfalls in the use of commercial nonionic detergents for the solubilization of integral membrane pro¬teins: Sulfhydryl oxidizing contaminants and their elimination
    • Chang, H. W., and Bock, E. (1980) Pitfalls in the use of commercial nonionic detergents for the solubilization of integral membrane pro¬teins: sulfhydryl oxidizing contaminants and their elimination, Anal Biochem 104, 112-117.
    • (1980) Anal Biochem , vol.104 , pp. 112-117
    • Chang, H.W.1    Bock, E.2
  • 39
    • 46749154714 scopus 로고    scopus 로고
    • Rapid preparation of nuclei-depleted deter¬gent-resistant membrane fractions suitable for proteomics analysis
    • Adam, R. M., Yang, W., Di Vizio, D., Mukhopadhyay, N. K., and Steen, H. (2008) Rapid preparation of nuclei-depleted deter¬gent-resistant membrane fractions suitable for proteomics analysis, BMC Cell Biol 9, 30.
    • (2008) BMC Cell Biol , vol.9 , pp. 30
    • Adam, R.M.1    Yang, W.2    Di Vizio, D.3    Mukhopadhyay, N.K.4    Steen, H.5
  • 40
    • 0018167317 scopus 로고
    • Retrieval and recycling of synaptic vesicle membrane in pinched-off nerve terminals (synaptosomes)
    • DOI 10.1083/jcb.78.3.685
    • Fried, R. C., and Blaustein, M. P. (1978) Retrieval and recycling of synaptic vesicle membrane in pinched-off nerve terminals (syn-aptosomes), J Cell Biol 78, 685-700. (Pubitemid 9007414)
    • (1978) Journal of Cell Biology , vol.78 , Issue.3 , pp. 685-700
    • Fried, R.C.1    Blaustein, M.P.2
  • 41
    • 0000745994 scopus 로고
    • Isolierung and Kristallisation des Garungsferment
    • Warburg, O., and Christian, W. (1941) Isolierung and Kristallisation des Garungsferment, Biochem Z 310, 384-421.
    • (1941) Biochem Z , vol.310 , pp. 384-421
    • Warburg, O.1    Christian, W.2


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