Electron Tomography and Immunogold Labelling as Tools to Analyse De Novo Assembly of Plant Cell Walls

Methods in Molecular Biology

Volumn 715, Issue , 2011, Pages 123-140

  Otegui, Marisa S ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Cell walls; Cryofixation; Cytokinesis; Electron tomography; Immunolabelling

Indexed keywords

ARABIDOPSIS; ARTICLE; CELL ORGANELLE; CELL WALL; COMPUTER PROGRAM; COMPUTER SIMULATION; CULTURE TECHNIQUE; CYTOLOGY; CYTOPLASM VESICLE; ELECTRON TOMOGRAPHY; GROWTH, DEVELOPMENT AND AGING; HISTOLOGY; IMAGE PROCESSING; IMMUNOHISTOCHEMISTRY; INSTRUMENTATION; METABOLISM; METHODOLOGY; MICROFILAMENT; MICROTUBULE; ORGANELLE SIZE; SEEDLING; ULTRASTRUCTURE;

ARABIDOPSIS; CELL WALL; COMPUTER SIMULATION; CULTURE TECHNIQUES; CYTOPLASMIC VESICLES; ELECTRON MICROSCOPE TOMOGRAPHY; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; MICROFILAMENTS; MICROTUBULES; ORGANELLE SIZE; ORGANELLES; PLASTIC EMBEDDING; SEEDLING; SOFTWARE;

EID: 79957734864     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-008-9_9     Document Type: Chapter

References (27)

1. Otegui, M. S., Mastronarde, D. N., Kang, B. H., Bednarek, S. Y., and Staehelin, L. A. (2001) Three-dimensional analysis of syncytial-type cell plates during endosperm cellularization visualised by high resolution electron tomography. Plant Cell 13, 2033–2051.
2. Otegui, M. S., and Staehelin, L. A. (2004) Electron tomographic analysis of post-meiotic cytokinesis during pollen development in Arabidopsis thaliana. Planta 218, 501–515.
3. Segui-Simarro, J. M., Austin, J. R., II, White, E. A., and Staehelin, L. A. (2004) Electron tomographic analysis of somatic cell plate formation in meristematic cells of Arabidopsis preserved by high-pressure freezing. Plant Cell 16, 836–856.
4. Austin, J. R., II, Segui-Simarro, J. M., and Staehelin, L. A. (2005) Quantitative analysis of changes in spatial distribution and plus-end geometry of microtubules involved in plant-cell cytokinesis. J Cell Sci 118, 3895–3903.
5. Otegui, M. S., and Austin, J. R., II (2007) Visualization of membrane-cytoskeletal interactions during plant cytokinesis. Methods Cell Biol 79, 221–240.
6. Mastronarde, D. N. (1997) Dual-axis tomography: an approach with alignment methods that preserve resolution. J Struct Biol 120, 343–352.
7. Mastronarde, D. N. (2005) Automated electron microscope tomography using robust prediction of specimen movements. J Struct Biol 152, 36–51.
8. Mastronarde, D. N. (2008) Correction for non-perpendicularity of beam and tilt axis in tomographic reconstructions with the IMOD package. J Microsc 230, 212–217.
9. Kremer, J. R., Mastronarde, D. N., and McIntosh, J. R. (1996) Computer visualization of three-dimensional image data using IMOD. J Struct Biol 116, 71–76.
10. Beemster, G. T. S., and Baskin, T. I. (1998) Analysis of cell division and elongation underlying the developmental acceleration of root growth in Arabidopsis thaliana. Plant Physiol 116, 1515–1526.
11. Otegui, M. S., and Staehelin, L. A. (2000) Cytokinesis in flowering plants: more than one way to divide a cell. Curr Opin Plant Biol 3, 493–502.
12. Otegui, M. S., and Staehelin, L. A. (2000) Syncytial-type cell plates: a novel kind of cell plate involved in endosperm cellularization of Arabidopsis. Plant Cell 12, 933–947.
13. Nagata, T., Nemoto, Y., and Hasezawa, S. (1992) Tobacco BY-2 cell line as the “HeLa” cell in the cell biology of higher plants. Int Rev Cytol 132, 1–30.
14. Nagata, T., and Kumagai, F. (1999) Plant cell biology through the window of the highly synchronised tobacco BY-2 cell line. Methods Cell Sci 21, 123–127.
15. Kakimoto, T., and Shibaoka, H. (1988) Cytoskeletal ultrastructure of phragmoplast-nuclei complexes isolated from cultured tobacco cells. Protoplasma S2, 95–103.
16. Samuels, L. A., Giddings, T. H., and Staehelin, L. A. (1995) Cytokinesis in tobacco BY-2 and root tip cells: a new model of cell plate formation in higher plants. J Cell Biol 130, 1345–1357.
17. Giddings, T. H. (2003) Freeze-substitution protocols for improved visualization of membranes in high-pressure frozen samples. J Microsc 212, 53–61.
18. Moore, P. J., Darvill, A. G., Albersheim, P., and Staehelin, L. A. (1986) Immunogold localization of xyloglucan and rhamnogalac-turonan I in the cell walls of suspension-cultured sycamore cells. Plant Physiol 82, 787–794.
19. Puhlmann, J., Bucheli, E., Swain, M. J., Dunning, N., Albersheim, P., Darvill, A. G., and Hahn, M.G. (1994) Generation of monoclonal antibodies against plant cell-wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1®2)-linked fucosyl-containing epitope. Plant Physiol 104, 699–710.
20. Knox, J. P., Linstead, P. J., Peart, J., Cooper, C., and Roberts, K. (1991) Developmentally regulated epitopes of cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J 1, 317–326.
21. Smallwood, M., Yates, E. A., Willats, W. G. T., Martin, H., and Knox, J. P. (1996) Immunochemical comparison of membrane associated and secreted arabinogalactan-proteins in rice and carrot. Planta 198, 452–459.
22. McEwen, B. F., and Frank, J. (2001) Electron tomographic and other approaches for imaging molecular machines. Curr Op Neurobiol 11, 594–600.
23. Marsh, B. J. (2005) Lessons from tomographic studies of the mammalian Golgi. Biochim Biophys Acta 1744, 273–292.
24. O’Toole, E. T., Giddings, J. T. H., Dutcher, S. K., and McIntosh, J. R. (2007) Understanding microtubule organizing centers by comparing mutant and wild type structures with electron tomography. Methods Cell Biol 79, 125–143.
25. Ladinsky, M. S., Mastronarde, D. N., McIntosh, J. R., Howell, K. E., and Staehelin, L. A. (1999) Golgi structure in three dimensions: functional insights from the normal rat kidney cell. J Cell Biol 144, 1135–1149.
26. Marsh, B. J., Mastronarde, D. N., Buttle, K. F., Howell, K. E., and McIntosh, J. R. (2001) Organellar relationship in the Golgi region of the pancreatic beta cell line, HIT-T15, visualised by high-resolution electron tomography. Proc Natl Acad Sci U S A 98, 2399–2406.
27. Donohoe, B. S., Kang, B.-H., and Staehelin, L. A. (2007) Identification and characterization of COPIa-and COPIb-type vesicle classes associated with plant and algal Golgi. Proc Natl Acad Sci U S A 104, 163–168.