Separation of wild-type lipopolysaccharides by centrifugal partition chromatography: Isolation of smooth form species

Innate Immunity

Volumn 3, Issue 1, 1996, Pages 1-7

  Suda, Yasuo ^a   Shiyama, Takaaki ^a   Yasukochi, Takashi ^a   Kawano, Kazuhisa ^a   Brade, Helmut ^b   Rietschel, Ernst Th ^b   Kusumoto, Shoichi ^a  

^a OSAKA UNIVERSITY   (Japan)

^b RESEARCH CENTER BORSTEL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 79957721982     PISSN: 17534259     EISSN: 17534267     Source Type: Journal    
DOI: 10.1177/096805199600300101     Document Type: Article

References (26)

1. Rietschel E.Th., Kirikae T., Schade F.U.et al.Bacterial endotoxin: molecular relationships of structure to activity and function. FASEB J 1994; 8: 217-225.
2. Westphal O., Lüderitz O. Chemische Erforschung von Lipopolysacchariden Gram-negativer Bakterien. Angew Chem 1954; 66:407-417.
3. Imoto M., Yoshimura H., Sakaguchi N., Kusumoto S., Shiba T. Total synthesis of Escherichia coli lipid A. Tetrahedron Lett 1985; 26:1545-1548.
4. Shiba T., Kusumoto S. Chemical synthesis and biological activity of lipid A analogs. In: Rietschel E.Th. ed. Handbook of Endotoxin Vol. 1, Chemistry of Endotoxin. Amsterdam; Elsevier 1984; 284-307.
5. Galanos C., Lehmann V., Lüderitz O.et al.Endotoxic properties of chemically synthesized lipid A part structures. Comparison of synthetic lipid A precursor and synthetic analogues with biosynthetic lipid A precursor and free lipid A. Eur J Biochem 1984; 140:221-227.
6. Kotani S., Takada H., Tsujimoto M.et al.Synthetic lipid A with endotoxic and related biological activities comparable to those of a natural lipid A from an Escherichia coli Re-mutant. Infect Immun 1985; 49:225-237.
7. Hitchcock P.J., Brown T.M. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 1983; 154: 269-277.
8. Jann B., Reske K., Jann K. Heterogeneity of lipopolysaccharide. Analysis of polysaccharide chain lengths by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Eur J Biochem 1975; 60:239-246.
9. Palva ET, Mäkelä P.H. Lipopolysaccharide heterogeneity in Salmonella typhimurium analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Eur J Biochem 1980; 107:137-143.
10. Goldmann R.C., Leive L. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. Eur J Biochem 1980; 107: 145-153.
11. Lüderitz T., Brandenburg K., Seydel U., Roth A., Galanos C., Rietschel E.Th. Structural and physicochemical requirements of endotoxins for the activation of arachidonic and metabolism in mouse peritoneal macrophages in vitro. Eur J Biochem 1989; 179:11-16.
12. Flebbe L.M., Chapes S.K., Morrison D.C. Activation of C3H/HeJ macrophage tumoricidal activity and cytokine release by R-chemotype lipopolysaccharide preparations. Different effects of IFN-γ. J Immunol 1990; 145:1505-1511.
13. Kirikae T., Schade F.U., Kirikae F., Rietschel E.Th., Morrison D.C. Isolation of a macrophage-like cell line defective in binding of lipopolysaccharide. Influence of serum and lipopolysaccharide chain length on macrophage activation. J Immunol 1993; 151: 2742-2752.
14. Hopf U., Ramadori G., Möller B., Galanos C. Hepatocellular clearance function of bacterial lipopolysaccharides and free lipid A in mice with endotoxic shock. Am J Emerg Med 1984; 2: 13-19.
15. Jiao B., Freudenberg M.A., Galanos C. Characterization of the lipid A component of genuine smooth-form lipopolysaccharide. Eur J Biochem 1989; 180: 515-518.
16. Vukajlovich S.W., Morrison D.C. Conversion of lipopolysaccharides to molecular aggregates with reduced subunit heterogeneity demonstration of lipopolysaccharide responsiveness in endotoxin unresponsive C3H/HeJ splenocytes. J Immunol 1983; 130: 2804-2808.
17. Goldman R.C., Leive L. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. Eur J Biochem 1980; 107: 145-153.
18. Fischer W. Purification and fractionation of lipopolysaccharide from gram negative bacteria by hydrophobic interaction chromatography. Eur J Biochem 1990; 194:655-661.
19. Manthey C.L., Vogel S.N. Elimination of trace endotoxin protein from rough form chemotype LPS. J Endotoxin Res 1994; 1: 84-91.
20. Galanos C., Jiao B., Komuro T., Freudenberg M.A., Lüderitz O. Large-scale fractionation of S-form lipopolysaccharide from Salmonella abortus egui. J Chromatogr 1988; 440: 397-404.
21. Cazes J., ed. Centrifugal partition chromatography. 3rd International colloquium on centrifugal partition chromatography. J Liquid Chromatogr 1990; 13: 3559-3710.
22. Foucaylt A.P., Bousquet O., Goffic F.L., Cazes J. Countercurrent chromatography with a new centrifugal partition chromatographic system. J Liquid Chromatogr 1992; 15: 2721-2733.
23. Harada K. A new aspect of high speed countercurrent chromatography. Pharmacia 1989; 25: 551-556.
24. Galanos C., Lüderitz O., Westphal O. Preparation and properties of a standardized lipopolysaccharide from Salmonella abortus equi (Novo-pyrexal). Zbl Bakt Hyg I Abt Orig 1979; 243: 226-244.
25. Tsai C-M., Frasch C. E. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 1982; 119:115-119.
26. Suda Y., Kirikae T., Shiyama T.et al.Macrophage activation in response to S-form lipopolysaccharides (LPS) separated by centrifugal partition chromatography from wild-type LPS: Effects of the O-polysaccharide portion of LPS. Biochem Biophys Res Commun 1995; 210: 678-685.