Monoclonal Antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Plant Cell Walls

Methods in Molecular Biology

Volumn 715, Issue , 2011, Pages 103-113

  Hervé, Cécile ^a   Marcus, Susan E ^b   Knox, J Paul ^b  

^a STATION BIOLOGIQUE   (France)

^b UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

Carbohydrate binding module; Cell wall immunocytochemistry; Immunofluorescence microscopy; Monoclonal antibody; Pectate lyase; Plant cell walls

Indexed keywords

MONOCLONAL ANTIBODY; POLYSACCHARIDE;

ARTICLE; CELL WALL; CHEMISTRY; FLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; MICROTOMY; PLANT;

ANTIBODIES, MONOCLONAL; CELL WALL; IMMUNOHISTOCHEMISTRY; MICROSCOPY, FLUORESCENCE; MICROTOMY; PLANTS; POLYSACCHARIDES;

EID: 79957687379     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-008-9_7     Document Type: Chapter

References (9)

1. Knox, J. P. (2008) Revealing the structural and functional diversity of plant cell walls. Curr Opin Plant Biol 11, 308–313.
2. Boraston, A. B., Bolam, D. N., Gilbert, H. J., and Davies, G. J. (2004) Carbohydrate-binding modules: fine-tuning polysaccharide recognition. Biochem J 382, 769–781.
3. McCartney, L., Gilbert, H. J., Bolam, D. N., Boraston, A. B., and Knox, J. P. (2004) Glycoside hydrolase carbohydrate-binding modules as molecular probes for the analysis of plant cell wall polymers. Anal Biochem 326, 49–54.
4. Marcus, S. E., Verhertbruggen, Y., Hervé, C., Ordaz-Ortiz, J. J., Farkas, V., Pedersen, H. L., Willats, W. G. T., and Knox, J. P. (2008) Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls. BMC Plant Biol 8, 60.
5. Hervé, C., Rogowski, A., Gilbert, H. J., and Knox, J. P. (2009) Enzymatic treatments reveal differential capacities for xylan recognition and degradation in primary and secondary plant cell walls. Plant J 58, 413–422.
6. Willats, W. G. T., Steele-King, C. G., Marcus, S. E., and Knox, J. P. (2002) Antibody techniques. In: Molecular Plant Biology – Volume Two: A Practical Approach (Gilmartin P. M., Bowler C. (eds)), pp 199–219, Oxford University Press, Oxford, UK.
7. Moller, I., Sørensen, I., Bernal, A. J., Blaukopf, C., Lee, K., Øbro, J., Pettolino, F., Roberts, A., Mikkelsen, J. D., Knox, J. P., Bacic, A., and Willats, W. G. T. (2007) High-throughput mapping of cell wall polymers within and between plants using novel microarrays. Plant J 50, 1118–1128.
8. Steedman, H. F. (1957) A new ribboning embedding medium for histology. Nature 179, 1345.
9. Meloche, C. G., Knox, J. P., and Vaughn, K. C. (2007) A cortical band of gelatinous fibers causes the coiling of redvine tendrils: a model based upon cytochemical and immunocy-tochemical studies. Planta 225, 485–498.