Natural plant extract tubeimoside I induces cytotoxicity via the mitochondrial pathway in human normal liver cells

Molecular Medicine Reports

Volumn 4, Issue 4, 2011, Pages 713-718

  Wang, Yashu ^a   Deng, Linhong ^a   Wang, Yajie ^b   Zhong, Hongzhe ^c   Jiang, Xuemei ^a   Chen, Jun ^a  

^a CHONGQING UNIVERSITY   (China)

^b No 324 Hospital of People's Liberation Army   (China)

^c Liaoning Blood Center   (China)

Author keywords

Apoptosis; Cytotoxicity; Human normal liver L 02 cell; Mitochondria; Tubeimoside I

Indexed keywords

CASPASE 3; CASPASE 9; CYTOCHROME C; CYTOTOXIC AGENT; PLANT EXTRACT; PROTEIN BAX; PROTEIN BCL 2; TUBEIMOSIDE I; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; BOLBOSTEMMA PANICULATUM; CELL ASSAY; CELL LINE; CELL MEMBRANE DEPOLARIZATION; CELL NUCLEUS; CELL PROLIFERATION; CELL STRUCTURE; CELL SURVIVAL; CONCENTRATION RESPONSE; CONTROLLED STUDY; CUCUMBER; CYTOSOL; CYTOTOXICITY TEST; DOSE TIME EFFECT RELATION; DOWN REGULATION; DRUG CYTOTOXICITY; ENZYME ACTIVATION; GROWTH INHIBITION; HUMAN; HUMAN CELL; LIVER CELL; LIVER TOXICITY; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; PROTEIN SECRETION; UPREGULATION;

APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; CASPASE 3; CASPASE 9; CELL LINE; CELL PROLIFERATION; CUCURBITACEAE; CYTOCHROMES C; HEPATOCYTES; HUMANS; MEDICINE, CHINESE TRADITIONAL; MITOCHONDRIA; PROTO-ONCOGENE PROTEINS C-BCL-2; SAPONINS; TRITERPENES;

BOLBOSTEMMA PANICULATUM; CUCURBITACEAE;

EID: 79957633120     PISSN: 17912997     EISSN: 17913004     Source Type: Journal    
DOI: 10.3892/mmr.2011.483     Document Type: Article

References (16)

1. Zhao XM: Supplement to the Compendium of Materia Medica (reprinted from the 1765 wood-engraved edition in Chinese). The People's Medical Publishing House, Beijing, p123, 1983.
2. Yu L, Ma R, Wang Y and Nishino H: Potent anti-tumor activity and low toxicity of tubeimoside I isolated from Bolbostemma paniculatum. Planta Med 60: 204-208, 1994. (Pubitemid 24207709)
3. Kong F, Zhu D, Xu R, et al: Structural study of tubeimoside I, a constituent of tubeimo. Tetrahedron Lett 27: 5765-5775, 1986.
4. Wang YQ, Yu LJ and Zhu JL: Studies on antitumor action of extracts of Bolbostemma paniculatum (Maxim.) Franquet. Journal of Shaanxi Med (in Chinese) 10: 55-59, 1981.
5. Ma RD, Yu LJ and Su WM: Induction of cell cycle arrest and apoptosis by tubeimoside I isolated from Bolbostemma paniculatum in HeLa cells. Chin J Clin Pharmacol Ther 9: 261, 2004.
6. Ma RD, Yu LJ and Yu TX: The inhibitory effects of TBMS I on tumorigenesis, progression and metastasis, and the associated mechanisms and targets. China Science and Technology Achievements 11: 39-43, 2010.
7. Ma RD, Song G, You WB, et al: Anti-microtubule activity of tubeimoside I and its colchicine binding site of tubulin. Cancer Chemoth Pharm 62: 559-568, 2008.
8. Xu Y, Chiu LF, He QY and Chen F: Tubeimoside-1 exerts cytotoxicity in HeLa cells through mitochondrial dysfunction and endoplasmic reticulum stress pathways. J Proteome Res 8: 1585-1593, 2009.
9. Wang F, Ma RD and Yu LJ: Role of mitochondria and mitochondrial cytochrome c in tubeimoside I-mediated apoptosis of human cervical carcinoma HeLa cell line. Cancer Chemoth Pharm 57: 389-399, 2006.
10. Wang YQ, Yu LJ and Yang SY: The absorption, distribution, metabolism and evacuation of tubeimoside I in animal model. J Chin Herbal Med 25: 256-258, 1994.
11. Zou H, Henzel WJ, Liu X, Lutschg A and Wang X: Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90: 405-413, 1997.
12. Li H, Zhu H, Xu CJ and Yuan J: Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell 94: 491-501, 1998. (Pubitemid 28391865)
13. Certo M, Moore VD, Nishino M, et al: Mitochondria primed by death signals determine cellular addiction to antiapoptotic BCL-2 family members. Cancer Cell 9: 351-365, 2006. (Pubitemid 43668734)
14. Nakazawa Y, Kamijo T, Koike K and Noda T: ARF tumor suppressor induces mitochondria-dependent apoptosis by modulation of mitochondrial Bcl-2 family proteins. J Biol Chem 278: 27888-27895, 2003. (Pubitemid 36899981)
15. Antonsson B, Conti F, Ciavatta A, et al: Inhibition of Bax channel-forming activity by Bcl-2. Science 277: 370-372, 1997. (Pubitemid 27450709)
16. Lanave C, Santamaria M and Saccone C: Comparative genomics: the evolutionary history of the Bcl-2 family. Gene 333: 71-79, 2004. (Pubitemid 38737353)