Streamlined protein expression and purification using cleavable self-aggregating tags

Microbial Cell Factories

Volumn 10, Issue , 2011, Pages

  Xing, Lei ^a   Wu, Wei ^a   Zhou, Bihong ^a   Lin, Zhanglin ^a  

^a TSINGHUA UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ACID LIPASE; AMADORIASE II; INTEIN; PEPTIDE DERIVATIVE; UNCLASSIFIED DRUG; XYLAN 1,4 BETA XYLOSIDASE; AMADORIASE; EXO 1,4 BETA D XYLOSIDASE; EXO-1,4-BETA-D-XYLOSIDASE; HYBRID PROTEIN; OXIDOREDUCTASE; PEPTIDE; PEPTIDE 18A; TRIACYLGLYCEROL LIPASE;

ARTICLE; CENTRIFUGATION; COST; ESCHERICHIA COLI; FEASIBILITY STUDY; NONHUMAN; PROTEIN AGGREGATION; PROTEIN ASSEMBLY; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; QUANTUM YIELD; SCALE UP; TIME; AMINO ACID SEQUENCE; BIOSYNTHESIS; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS;

ESCHERICHIA COLI;

AMINO ACID OXIDOREDUCTASES; AMINO ACID SEQUENCE; ESCHERICHIA COLI; INTEINS; LIPASE; MOLECULAR SEQUENCE DATA; PEPTIDES; RECOMBINANT FUSION PROTEINS; XYLOSIDASES;

EID: 79957629066     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-10-42     Document Type: Article

References (23)

1. Banki MR, Wood DW. Inteins and affinity resin substitutes for protein purification and scale up. Microb Cell Fact 2005, 4(1):32. 10.1186/1475-2859-4-32, 1310522, 16283936.
2. Arnau J, Lauritzen C, Petersen GE, Pedersen J. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins. Protein Expr Purif 2006, 48(1):1-13. 10.1016/j.pep.2005.12.002, 16427311.
3. Achmuller C, Kaar W, Ahrer K, Wechner P, Hahn R, Werther F, Schmidinger H, Cserjan-Puschmann M, Clementschitsch F, Striedner G, Bayer K, Jungbauer A, Auer B. N(pro) fusion technology to produce proteins with authentic N termini in E. coli. Nat Methods 2007, 4(12):1037-1043. 10.1038/nmeth1116, 18026112.
4. Banki MR, Feng LA, Wood DW. Simple bioseparations using self-cleaving elastin-like polypeptide tags. Nat Methods 2005, 2(9):659-661. 10.1038/nmeth787, 16074986.
5. Ge X, Yang DSC, Trabbic-Carlson K, Kim B, Chilkoti A, Filipe CDM. Self-cleavable stimulus responsive tags for protein purification without chromatography. J Am Chem Soc 2005, 127(32):11228-11229. 10.1021/ja0531125, 16089436.
6. Wu W, Xing L, Zhou B, Lin Z. Active protein aggregates induced by terminally attached self-assembling peptide ELK16 in Escherichia coli. Microb Cell Fact 2011, 10(1):9. 10.1186/1475-2859-10-9, 3045283, 21320350.
7. Ventura S, Villaverde A. Protein quality in bacterial inclusion bodies. Trends Biotechnol 2006, 24(4):179-185. 10.1016/j.tibtech.2006.02.007, 16503059.
8. Mitraki A. Protein aggregation: from inclusion bodies to amyloid and biomaterials. Adv Protein Chem Struct Biol 2010, 79:89-125.
9. Garcia-Fruitos E. Inclusion bodies: a new concept. Microb Cell Fact 2010, 9(1):80. 10.1186/1475-2859-9-80, 2987918, 21040537.
10. Vazquez E, Villaverde A. Engineering building blocks for self-assembling protein nanoparticles. Microb Cell Fact 2010, 9(1):101. 10.1186/1475-2859-9-101, 3022712, 21192790.
11. van Pouderoyen G, Eggert T, Jaeger KE, Dijkstra BW. The crystal structure of Bacillus subtilis lipase: A minimal alpha/beta hydrolase fold enzyme. J Mol Biol 2001, 309(1):215-226. 10.1006/jmbi.2001.4659, 11491291.
12. LaGrange DC, Pretorius IS, vanZyl WH. Cloning of the Bacillus pumilus beta-xylosidase gene (xynB) and its expression in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 1997, 47(3):262-266. 10.1007/s002530050924, 9114518.
13. Xu WZ, Shima Y, Negoro S, Urabe I. Sequence and properties of beta-xylosidase from Bacillus pumilus IPO-contradiction of the previous nucleotide-sequence. Eur J Biochem 1991, 202(3):1197-1203. 10.1111/j.1432-1033.1991.tb16490.x, 1765080.
14. Takahashi M, Pischetsrieder M, Monnier VM. Isolation, purification, and characterization of amadoriase isoenzymes (fructosyl amine-oxygen oxidoreductase EC 1.5.3) from Aspergillus sp. J Biol Chem 1997, 272(6):3437-3443. 10.1074/jbc.272.6.3437, 9013588.
15. Telenti A, Southworth M, Alcaide F, Daugelat S, Jacobs WR, Perler FB. The Mycobacterium xenopi GyrA protein splicing element: Characterization of a minimal. J Bacteriol 1997, 179(20):6378-6382. 179553, 9335286.
16. Wu H, Xu MQ, Liu XQ. Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein. Biochim Biophys Acta 1998, 1387(1-2):422-432. 10.1016/S0167-4838(98)00157-5, 9748659.
17. Sakaue R, Kajiyama N. Thermostabilization of bacterial fructosyl-amino acid oxidase by directed evolution. Appl Environ Microbiol 2003, 69(1):139-145. 10.1128/AEM.69.1.139-145.2002, 152437, 12513988.
18. Shao WL, Wiegel J. Purification and characterization of a thermostable beta-xylosidase from thermoanaerobacter-ethanolicus. J Bacteriol 1992, 174(18):5848-5853. 207117, 1522062.
19. Winkler UK, Stuckmann M. Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia-marcescens. J Bacteriol 1979, 138(3):663-670. 218088, 222724.
20. Kavoosi M, Creagh AL, Kilburn DG, Haynes CA. Strategy for selecting and characterizing linker peptides for CBM9-tagged fusion proteins expressed in Escherichia coli. Biotechnol Bioeng 2007, 98(3):599-610. 10.1002/bit.21396, 17394253.
21. Lesuisse E, Schanck K, Colson C. Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme. Eur J Biochem 1993, 216(1):155-160. 10.1111/j.1432-1033.1993.tb18127.x, 8396026.
22. Warburg O, Chiristian W. Isolierung und Kristallisation des Garungsterments Enolase. Biochem Z 1942, 310:384-421.
23. Scheich C, Sievert V, Bussow K. An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography. Bmc Biotechnol 2003, 3:12-19. 10.1186/1472-6750-3-12, 183854, 12885298.