Detection of pathogenic mycobacteria based on functionalized quantum dots coupled with immunomagnetic separation

PLoS ONE

Volumn 6, Issue 5, 2011, Pages

  Liandris, Emmanouil ^a   Gazouli, Maria ^b   Andreadou, Margarita ^a   Sechi, Leonardo A ^c   Rosu, Valentina ^c   Ikonomopoulos, John ^a  

^a AGRICULTURAL UNIVERSITY OF ATHENS   (Greece)

^b UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

^c UNIVERSITY OF SASSARI   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CADMIUM SELENIDE; QUANTUM DOT; BACTERIUM ANTIBODY; MICROSPHERE;

ARTICLE; BACTERIUM DETECTION; CONTROLLED STUDY; ESCHERICHIA COLI; IMMUNOMAGNETIC SEPARATION; LIMIT OF DETECTION; MYCOBACTERIUM BOVIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PROCESS DEVELOPMENT; SALMONELLA; SPECTROFLUOROMETRY; ANIMAL; CYTOLOGY; FLUORESCENCE MICROSCOPY; IMMUNOLOGY; ISOLATION AND PURIFICATION; MAGNETISM; METHODOLOGY; MOUSE; MYCOBACTERIUM;

BACTERIA (MICROORGANISMS); CORYNEBACTERINEAE; MYCOBACTERIUM BOVIS BCG; MYCOBACTERIUM TUBERCULOSIS; SALMONELLA;

ANIMALS; ANTIBODIES, BACTERIAL; ESCHERICHIA COLI; IMMUNOMAGNETIC SEPARATION; LIMIT OF DETECTION; MAGNETICS; MICE; MICROSCOPY, FLUORESCENCE; MICROSPHERES; MYCOBACTERIUM; MYCOBACTERIUM BOVIS; QUANTUM DOTS;

EID: 79957624642     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0020026     Document Type: Article

References (15)

1. Pfyffer GE, Brown-Elliott BA, Wallace RJ Jr, (2003) Mycobacterium: general characteristics, isolation, and staining procedures. In: Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH, editors. Manual of clinical microbiology, 8th ed. ASM Press pp. 532-559.
2. Lin PL, Yee J, Klein E, Lerche NW, (2008) Immunological concepts in tuberculosis diagnostics for non-human primates: a review. Med Primatol 37: 44-51.
3. Primm TP, Lucero CA, Falkinham JO, (2004) Health impacts of environmental mycobacteria. Clin Microbiol Rev 17: 98-106.
4. Serrano-Moreno BA, Romero TA, Arriaga C, Torres RA, Pereira-Suárez AL, et al. (2008) High frequency of Mycobacterium bovis DNA in colostra from tuberculous cattle detected by nested PCR. Zoonoses Public Health 55: 258-266.
5. Valanne A, Huopalahti S, Vainionpää R, Lövgren T, Härmä H, (2005) Rapid and sensitive HBsAg immunoassay based on fluorescent nanoparticle labels and time-resolved detection. J Virol Methods 129: 83-90.
6. Branen JR, Hass MJ, Douthit ER, Maki WC, Branen AL, (2007) Detection of Escherichia coli O157, Salmonella enterica serovar Typhimurium, and staphylococcal enterotoxin B in a single sample using enzymatic bio-nanotransduction. J Food Prot 70: 841-50.
7. Zhu L, Ang S, Liu WT, (2004) Quantum dots as a novel immunofluorescent detection system for Cryptosporidium parvum and Giardia lamblia. Appl Environ Microbiol 70: 597-8.
8. Chan WC, Nie S, (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281: 2016-2018.
9. Jaiswal JK, Mattoussi H, Mauro JM, Simon SM, (2003) Long-term multiple color imaging of live cells using quantum dot bioconjugates. Nat Biotechnol 21: 47-51.
10. Liandris E, Gazouli M, Andreadou M, Comor M, Abazovic N, et al. (2009) Direct detection of unamplified DNA from pathogenic mycobacteria using DNA-derivatized gold nanoparticles. J Microbiol Methods 78: 260-264.
11. Gazouli M, Liandris E, Andreadou M, Sechi LA, Masala S, et al. (2010) Specific detection of unamplified mycobacterial DNA by use of fluorescent semiconductor quantum dots and magnetic beads. J Clin Microbiol 48: 2830-2835.
12. Pethe K, Alonso S, Biet F, Delogu G, Brennan MJ, et al. (2001) The heparin-binding haemagglutinin of M. tuberculosis is required for extrapulmonary dissemination. Nat 412: 190-194.
13. Menozzi FD, Rouse JH, Alavi M, Laude-Sharp M, Muller J, et al. (1996) Identification of a heparin-binding hemagglutinin present in mycobacteria. J Exp Med 184: 993-1001.
14. Shin AR, Lee KS, Lee JS, Kim SY, Song CH, et al. (2006) Mycobacterium tuberculosis HBHA protein reacts strongly with the serum immunoglobulin M of tuberculosis patients. Clin Vaccine Immunol 13: 869-875.
15. Locht C, Hougardy JM, Rouanet C, Place S, Mascart F, (2006) Heparin-binding hemagglutinin, from an extrapulmonary dissemination factor to a powerful diagnostic and protective antigen against tuberculosis. Tuberculosis 86: 303-309.