Development of a recombinant ε-poly-L-lysine synthetase expression system to perform mutational analysis

Journal of Bioscience and Bioengineering

Volumn 111, Issue 6, 2011, Pages 646-649

  Yamanaka, Kazuya ^a   Kito, Naoko ^b   Kita, Akihiro ^b   Imokawa, Yuuki ^b   Maruyama, Chitose ^b   Utagawa, Takashi ^b   Hamano, Yoshimitsu ^b  

^a CHISSO CORPORATION   (Japan)

^b FUKUI PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Poly L lysine; Biopolymer; Membrane protein; Non ribosomal peptide synthetase; Streptomyces albulus

Indexed keywords

ADENYLATION; EXPRESSION SYSTEM; L-LYSINE; MEMBRANE PROTEIN; MEMBRANE PROTEINS; MUTATIONAL ANALYSIS; NONRIBOSOMAL PEPTIDE SYNTHETASES; POLY-L-LYSINE; STREPTOMYCES ALBULUS; SYNTHETASES; THIOLATION;

BIOLOGICAL MEMBRANES; PEPTIDES;

AMINO ACIDS;

EPSILON POLYLYSINE SYNTHETASE; POLYLYSINE; RECOMBINANT ENZYME; SYNTHETASE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; MUTATIONAL ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION;

CATALYSIS; FERMENTATION; MEMBRANE PROTEINS; MUTAGENESIS, SITE-DIRECTED; PEPTIDE BIOSYNTHESIS, NUCLEIC ACID-INDEPENDENT; PEPTIDE SYNTHASES; POLYLYSINE; POLYMERIZATION; SEQUENCE ANALYSIS, PROTEIN; STREPTOMYCES;

STREPTOMYCES ALBULUS;

EID: 79957609698     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.01.020     Document Type: Article

References (15)

1. Hamano Y. Amino-acid homopolymers occurring in nature. Microbiology monographs 2010, vol. 15:23-44. Springer, Heidelberg. Y. Hamano (Ed.).
2. Shima S., Sakai H. Polylysine produced by Streptomyces. Agric. Biol. Chem. 1977, 41:1807-1809.
3. Shima S., Sakai H. Poly-L-lysine produced by Streptomyces: II. Taxonomy and fermentation studies. Agric. Biol. Chem. 1981, 45:2497-2502.
4. Shima S., Sakai H. Poly-L-lysine produced by Streptomyces: III. Chemical studies. Agric. Biol. Chem. 1981, 45:2503-2508.
5. Shima S., Matsuoka H., Iwamoto T., Sakai H. Antimicrobial action of ε-poly-L-lysine. J. Antibiot. (Tokyo) 1984, 37:1449-1455.
6. Hiraki J., Hatakeyama M., Morita H., Izumi Y. Improved ε-poly-L-lysine production of an S-(2-aminoethyl)-L-cysteine resistant mutant of Streptomyces albulus. Seibutsu-kogaku 1998, 76:487-493. (in Japanese).
7. Yamanaka K., Maruyama C., Takagi H., Hamano Y. ε-poly-L-lysine dispersity is controlled by a highly unusual nonribosomal peptide synthetase. Nat. Chem. Biol. 2008, 4:766-772.
8. Marahiel M.A., Stachelhaus T., Mootz H.D. Modular peptide synthases involved in nonribosomal peptide synthesis. Chem. Rev. 1997, 97:2651-2673.
9. Mootz H.D., Schwarzer D., Marahiel M.A. Ways of assembling complex natural products on modular nonribosomal peptide synthetases. Chembiochem 2002, 3:490-504.
10. Schwarzer D., Finking R., Marahiel M.A. Nonribosomal peptides: from genes to products. Nat. Prod. Rep. 2003, 20:275-287.
11. Walsh C. Antibiotics: action, origins, resistance 2003, 195-218. ASM Press, Washington, D.C.
12. Hamano Y., Nicchu I., Hoshino Y., Kawai T., Nakamori S., Takagi H. Development of gene delivery systems for the ε-poly-L-lysine producer, Streptomyces albulus. J. Biosci. Bioeng. 2005, 99:636-641.
13. Yamanaka K., Kito N., Imokawa Y., Maruyama C., Utagawa T., Hamano Y. Mechanism of ε-poly-L-lysine production and accumulation revealed by identification and analysis of an ε-poly-L-lysine-degrading enzyme. Appl. Environ. Microbiol. 2010, 76:5669-5675.
14. Bibb M.J., Janssen G.R., Ward J.M. Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. Gene 1985, 38:215-226.
15. Hamano Y., Nicchu I., Shimizu T., Onji Y., Hiraki J., Takagi H. ε-Poly-L-lysine producer, Streptomyces albulus, has feedback-inhibition resistant aspartokinase. Appl. Microbiol. Biotechnol. 2007, 76:873-882.